Oral delivery of allopurinol niosomes in treatment of gout in animal model

Singh, Neelu; Parashar, Poonam; Tripathi, Chandra Bhushan; Kanoujia, Jovita; Kaithwas, Gaurav; Saraf, Shubhini A.

doi:10.1080/08982104.2016.1174943

Cited by 27 publications

(14 citation statements)

References 19 publications

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“…The modified NPs displayed a minor decrease in %EE which could be due to the loss of free drug from the surface of unmodified NPs during conjugation with activated biotin. The formulations exhibited a slow release in the later stage as the polymer erodes slowly at pH 6.8, and the drug is available for dissolution only after the erosion of polymeric layers [39]. Sustained release at a relative pH of a tumour is a desirable characteristic for drug delivery to the tumour as relatively acidic microenvironment has been observed inside tumours which might facilitate site-specific delivery [40].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Biotinylated naringenin intensified anticancer effect of gefitinib in urethane-induced lung cancer in rats: favourable modulation of apoptotic regulators and serum metabolomics

Parashar

Tripathi

Arya

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Co-therapy through biotin modified nanoparticles (NPs) of gefitinib (Gnb) and naringenin (Nar) was investigated for its therapeutic and synergistic potential against lung cancer. The biotin-conjugated polymeric NPs (bty-Nar/Gnb) were developed using oil in water emulsion technique and optimized using central composite design. The formulations were subjected to various in vitro (A549 cell lines) and in vivo evaluations in urethane-induced lung cancer. Co-administration of Gnb and Nar NPs displayed a significant reduction in tumour volume while restoring the biochemical parameters and serum metabolites to normal levels. Significant down-regulation of anti-apoptotic proteins (P-16, MMP-9 and Bcl-2) and upregulation of pro-apoptotic proteins (caspase-9 and BAX) was displayed with co-therapy. This investigation demonstrated the superiority of co-therapy over individual therapy for improved therapeutic efficacy and is favourable for developing a safe, effective and targeted delivery for lung carcinoma therapy.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Biotinylated naringenin intensified anticancer effect of gefitinib in urethane-induced lung cancer in rats: favourable modulation of apoptotic regulators and serum metabolomics

Parashar

Tripathi

Arya

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…In Table 2 , it was observed and concluded that the Higuchi model gave the highest value of the squared correlation coefficient (R2), indicating that this mathematical model was most suitable for describing the release of the NAR from PCL nanoparticles. These results suggest that the release of NAR from PCL nanoparticles is controlled by diffusion [ 37 ]. From these results, the mechanism of the drug release from PCL nanoparticles can be considered to follow the sequence: (1) dissolution media penetrate into polymeric matrix of the nanoparticles through pores; (2) slowly dissolve the drug; and (3) NAR is released by diffusion in the acceptor solution.…”

Section: Resultsmentioning

confidence: 99%

Hyaluronic Acid Decorated Naringenin Nanoparticles: Appraisal of Chemopreventive and Curative Potential for Lung Cancer

et al. 2018

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Lung carcinoma is the most common cancer in men and second in women (preceded by breast cancer) worldwide. Around 1 in 10 of all cancers diagnosed in men, lung cancer contributed to a total fraction of 20% cancer deaths. Naringenin (NAR) is well known for its chemopreventive properties since ancient times but lacks an appropriate delivery carrier. The objective of present study was to expand the functionality of naringenin loaded poly caprolactone (PCL) nanoparticles in terms of release, chemoprevention and therapeutics. Polymeric nanoparticles such as PCL lack target specificity; hence, surface modification was attempted using layer by layer technique (LBL) to achieve improved and desired delivery as well as target specificity. The designing of Hyaluronic acid (HA) decorated PCL nanoparticles were prepared by utilizing self-assembling LBL technique, where a polycationic layer of a polymer was used as a linker for modification between two polyanionic layers. Additionally, an attempt has been made to strengthen the therapeutic efficacy of PCL nanocarriers by active targeting and overcoming the extracellular matrix associated barriers of tumors using HA targeting cluster determinant 44 receptor (CD44). Cell cytotoxicity study on A549 cells and J774 macrophage cells depicted enhanced anticancer effect of NAR-HA@CH-PCL-NP with safe profile on macrophages. Uptake study on A549 cells advocated enhanced drug uptake by cancer cells. Cell cycle arrest analysis (A549 cell lines) demonstrated the superior cytotoxic effect and active targeting of NAR-HA@CH-PCL-NP. Further chemopreventive treatment with NAR-HA@CH-PCL-NP was found effective in tumor growth inhibitory effect against urethane-induced lung cancer in rat. In conclusion, developed formulation possesses a promising potential as a therapeutic and chemopreventive agent against urethane-induced lung carcinoma in albino wistar rats.

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“…It was not possible to obtain histological samples from vasculature of living individuals in this study. While several animal models for gout are available[ 37 - 40 ], most involve injecting MSU crystals within the joints of animals, and to our knowledge, no animal models for vascular urate deposition exist. We have attempted to validate the current approach by scanning ex vivo urinary calculi and in vitro exams of crystalline MSU and calcium with reasonable results (See supplementary material).…”

Section: Discussionmentioning

confidence: 99%

Quantification of uric acid in vasculature of patients with gout using dual-energy computed tomography

Barazani

Chi

Pyzik

et al. 2020

WJR

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BACKGROUND Gout, caused by hyperuricemia and subsequent deposition of aggregated monosodium urate crystals (MSU) in the joints or extra-articular regions, is the most common inflammatory arthritis. There is increasing evidence that gout is an independent risk factor for hypertension, cardiovascular disease progression and mortality. AIM To evaluate if dual energy computed tomography (DECT) could identify MSU within vessel walls of gout patients, and if MSU deposits within the vasculature differed between patients with gout and controls. This study may help elucidate why individuals with gout have increased risk for cardiovascular disease. METHODS 31 gout patients and 18 controls underwent DECT scans of the chest and abdomen. A material decomposition algorithm was used to distinguish regions of MSU (coded green), and calcifications (coded purple) from soft tissue (uncoded). Volume of green regions was calculated using a semi-automated volume assessment program. Between-group differences were analyzed using Mann-Whitney U exact test and nonparametric rank regression. RESULTS Gout patients had significantly higher volume of MSU within the aorta compared to controls [Median (Min-Max) of 43.9 (0-1113.5) vs 2.9 (0-219.4), P = 0.01]. Number of deposits was higher in gout patients compared to controls [Median (Min-Max) of 20 (0-739) vs 1.5 (0-104), P = 0.008]. However, the difference was insignificant after adjustment for age, gender, history of cardiovascular disease and diabetes. Increased age was positively associated with total urate volume ( r s = 0.64; 95% confidence interval: 0.43-0.78). CONCLUSION This pilot study showed that DECT can quantify vascular urate deposits with variation across groups, with gout patients possibly having higher deposition. This relationship disappeared when adjusted for age, and there was a positive relationship between age and MSU deposition. While this study does not prove that green coded regions are truly MSU deposition, it corroborates recent studies that show the presence of vascular deposition.

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Oral delivery of allopurinol niosomes in treatment of gout in animal model

Abstract: This study reveals that niosomes can be an efficient delivery system for the treatment of gout.

Cited by 27 publications

References 19 publications

RETRACTED ARTICLE: Biotinylated naringenin intensified anticancer effect of gefitinib in urethane-induced lung cancer in rats: favourable modulation of apoptotic regulators and serum metabolomics

RETRACTED ARTICLE: Biotinylated naringenin intensified anticancer effect of gefitinib in urethane-induced lung cancer in rats: favourable modulation of apoptotic regulators and serum metabolomics

Hyaluronic Acid Decorated Naringenin Nanoparticles: Appraisal of Chemopreventive and Curative Potential for Lung Cancer

Quantification of uric acid in vasculature of patients with gout using dual-energy computed tomography

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