Oral Delivery of DNA Vector Conjugated with Chitosan and Its Effect on Th1 Polarized Inflammation

Choi, B. C.; Choi, Juyoung; Jo, D; Sohn, Seonghyang

doi:10.5772/20455

Cited by 2 publications

(4 citation statements)

References 27 publications

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“…UV-inactivated HSV-1 is incapable of transcribing its early genes, leading to an inability of the virus to replicate [53]. In addition, we have confirmed that chitosan-pcDNA-EGFP-mIL4 DNA vector mixture can improve HSV-1 induced Behcet's disease-like symptoms by increasing IL-4 cytokine [27]. …”

Section: Discussionmentioning

confidence: 69%

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Chitosan as an Immunomodulating Adjuvant on T-Cells and Antigen-Presenting Cells in Herpes Simplex Virus Type 1 Infection

Choi

Anower

et al. 2016

Mediators of Inflammation

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Herpes disease caused by herpes simplex virus type 1 (HSV-1) is an intractable condition. It is a major concern in public health. Our purpose of this study was to verify the function of chitosan as an adjuvant for immune regulation specifically under herpes simplex virus type 1 (HSV-1) infection. Ahead of HSV infection, chitosan, heat inactivated green fluorescent protein expressing HSV (G-HSV), and a combination of chitosan and G-HSV were used to pretreat ICR mice followed by HSV-1 infection. Using flow cytometric analysis, the frequencies of T-cells, monocytes, dendritic cells (DCs), and natural killer (NK) cells were analyzed by surface expression of CD4+, CD8+, CD14+, CD11c+, NK1.1+, and DX5+ cells. In HSV infected mice, chitosan treatment significantly increased the frequencies of CD4+ T-cells (33.6 ± 5.78%) compared to those in the control group (24.02 ± 12.47%, p = 0.05). The frequencies of DC and NK cells were also significantly different between chitosan treated mice and control mice. In addition, anti-HSV IgG antibody was downregulated in chitosan treated mice. These results suggest that chitosan is a potential modulator or immune stimulator as an adjuvant in HSV-1 infected mice.

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Section: Discussionmentioning

confidence: 69%

“…In our previous results, treatment with an oral chitosan-pCIN-mIL-4 mixture was found to lead to expression of IL-4 mRNA and protein in intestinal tissues and increased serum levels of IL-4 in mice. It has been reported that chitosan encapsulated pDNA enables effective transfer of GFP gene into cells in vivo [27]. …”

Section: Introductionmentioning

confidence: 99%

Chitosan as an Immunomodulating Adjuvant on T-Cells and Antigen-Presenting Cells in Herpes Simplex Virus Type 1 Infection

Choi

Anower

et al. 2016

Mediators of Inflammation

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“…Chitosan is a safe and effective adjuvant with many biological effects on drug delivery [ 46 ]. A mixture of chitosan and pCIN-mIL-4 DNA vector can significantly increase IL-4 mRNA and IL-4 protein levels after in vivo mouse administration [ 47 ]. Oral administration of pCIN-mIL-4 DNA vector in combination with chitosan has effectively delivered DNA vector to intestinal tissues of mice.…”

Section: Therapeutic Applications Of Inflammatory Mice Model Of Bementioning

confidence: 99%

“…Subcutaneous injection of the IL-4-expressing vector by gene gun bombardment also improves BD symptoms in mice by enhancing serum IL-4, which alters the Th1 response toward the Th2 response [ 51 ]. Increase of mRNA and protein levels of IL-4 were also observed after the administration of a mixture of chitosan and pCIN-mIL-4 in mice [ 47 ].…”

Section: Therapeutic Applications Of Inflammatory Mice Model Of Bementioning

confidence: 99%

HSV-Induced Systemic Inflammation as an Animal Model for Behçet’s Disease and Therapeutic Applications

Islam

Sohn

2018

Viruses

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Behçet’s disease (BD) affects multiple organs. It is mainly characterized by recurrent oral, skin, and genital aphthous ulcers, and eye involvement. Successful management of BD is increasing, although its etiology remains unclear. A number of etiologies have been proposed, including environmental, genetic, viral, and immunological factors. To understand its complex etiology and improve its management, animal models of BD have been used to enable more effective therapeutic applications with increased clinical significance. An herpes simplex virus (HSV) type 1-induced BD mouse model has shown disease characteristics similar to those seen in BD patients. An HSV-induced BD animal model has been used to test various therapeutic modalities. The applied modalities are several materials that are derived from natural products, conventional therapeutics, and possible biologics. In this review, we provided how they regulate inflammation in an HSV-induced BD model.

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Oral Delivery of DNA Vector Conjugated with Chitosan and Its Effect on Th1 Polarized Inflammation

Cited by 2 publications

References 27 publications

Chitosan as an Immunomodulating Adjuvant on T-Cells and Antigen-Presenting Cells in Herpes Simplex Virus Type 1 Infection

Chitosan as an Immunomodulating Adjuvant on T-Cells and Antigen-Presenting Cells in Herpes Simplex Virus Type 1 Infection

HSV-Induced Systemic Inflammation as an Animal Model for Behçet’s Disease and Therapeutic Applications

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