Oral Epidermal Growth Factor Is Trophic for the Stomach in the Neonatal Rat

Falconer, John

doi:10.1159/000242730

Cited by 29 publications

(5 citation statements)

References 10 publications

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“…EGF ad ministered orally to suckling rats 3 times daily for 2 days resulted in an increased height of the antral mucosa when high doses were used, whereas the height of the fundic or ileal muco sa was unaffected [12]. Neonatal rats fed a for mula containing EGF displayed an increased weight of the stomach [13], Cultured gastric fundic epithelial cells from adult rabbits were forced into proliferation in a dose-dependent manner by EGF [ 14]. EGF has also previously been proposed to exert a regulatory influence on gastric acid secretion [15,16], It seems as if EGF in the stomach may transiently influence physiological functions such as gastric acid secretion and regeneration of the mucosa.…”

Section: Discussionmentioning

confidence: 99%

Epidermal Growth Factor Induces Increased Mucosal Thickness of the Small Intestine in Mouse

Jansen

Ihse²,

Axelson³

1997

Eur Surg Res

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Epidermal growth factor (EGF) is known to exert a mitogenic effect in different tissues, including the digestive tract. The aim of the present study was to evaluate whether long-term infusion of EGF causes trophic effects in the gastrointestinal tract of female mice. The animals were infused subcutaneously in the neck with human recombinant EGF in a dose of 10 µg/kg/h (1.6 nmol/kg/h) using an osmotic minipump for 1, 3 and 7 days, respectively. Tritiated thymidine was continuously infused intraperitoneally during the same period, except in the 7-day group, where it was infused during the last 3 days. The mucosal thickness was measured microscopically. As a measurement of DNA synthesis, the amount of thymidine retained in the mucosa was registered using a scintillation counter. After 1 day of EGF infusion, the mucosal thickness was increased in the antrum and, after 3 days, in the fundus. In the proximal duodenum, an increased depth of the crypts was seen after 1 day, followed by increased villi height after 3 and 7 days; in the distal duodenum, EGF evoked increased villi height after 3 and 7 days. The height of villi was increased after 7 days in the jejunum and ileum in the EGF-treated animals. The tritium incorporation was increased in the fundus of the stomach and the proximal duodenum in the EGF-treated animals after 3 days, whereas no significant increase in tritiated thymidine incorporation could be detected in the EGF-treated animals after 1 and 7 days compared to the controls. In conclusion, continuous infusion of EGF evoked increased mucosal thickness in the small intestine, while the trophic effects were only of a short duration in the stomach and absent in the colon.

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Section: Discussionmentioning

confidence: 99%

Epidermal Growth Factor Induces Increased Mucosal Thickness of the Small Intestine in Mouse

Jansen

Ihse²,

Axelson³

1997

Eur Surg Res

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“…approximately 30 days during late gestation, in the guinea pig [16][17][18][19] contrasts with the much shorter periods exhibited by altricial species: 4 days in the rat [20] and 5 days in the mouse [21]. These altricial spe cies show further changes in gastrointestinal morphology and physiology during the suck ling period and at weaning [6], Newborn rats fed for 40 h on an artificial formula isocaloric with expressed rat milk had significantly lighter stomachs and intes tines, which contained less DNA and RNA than those fed breast milk [4], It has been suggested that epidermal growth factor in natural milk may account for these differ ences in gastrointestinal growth [22,23], En teral feeding of rats with an artificial formula by gastrostomy from the 5th postnatal day until weaning led to initial deficit in gas trointestinal growth followed by enhanced growth of stomach, small intestine and cae cum [24]. Such findings were also reported when suckling rats were reared on an artifi cial milk from the 12th to 16th postnatal day, though reduction in caloric intake of feeds led to small intestinal growth compara ble with mother-fed rats, together with an inferior rate of body growth [25].…”

Section: Discussionmentioning

confidence: 99%

Neonatal Gastrointestinal Growth and Function: Are They Regulated by Composition of Feeds?

Weaver

Landymore-Lim

Lucas³

1991

Neonatology

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The effects of natural suckling versus a cow’s milk formula on gastrointestinal growth and mucosal function were measured in newborn guinea pigs during the first 8 days of postnatal life. Comparable growth was achieved between the two groups. Both feeds appeared to be a stimulus for the growth of the gastrointestinal tract and expression of disaccharidases, but not for mucosal growth. Formula feeding was associated with a diminished rate of lactase expression and an enhanced rate of maltase and sucrase expression suggesting that the effect of natural suckling is regulatory, acting to delay expression of an inherent ontogenic programme uncovered by formula feeding. These findings in the precocial guinea pig contrast with those in the altricial rat, and have implications for our understanding of the regulation of gastrointestinal development and effects of formula feeds, in other species, including man.

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“…Epidermal growth factor (EGF) is present in the milk of animals of several species (Beardmore and Richards 1983;Moran et al 1983;Jaeger 1987;Schaudies et al 1990) including that of mares (Murray et al 1992). EGF has gastrointestinal trophic effects in neonatal rodents (Berseth 1987;Falconer 1987). EGF has a pronounced effect on keratinocytes in different cell systems (Boonstra ef al.…”

Section: Discussionmentioning

confidence: 99%

Age‐related characteristics of gastric squamous epithelial mucosa in foals

Murray

Mahaffey

1993

Equine Veterinary Journal

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Summary Specimens of grossly normal gastric stratified squamous epithelial mucosa adjacent to the margo plicatus on the right side and along the greater curvature were obtained from 7 foetuses, 10 term foals and 12 foals 2–35 days old. Gastric squamous epithelium from the foetuses changed markedly during gestation. At 150–270 days, the epithelium was 8–10 cells thick, with a single layer of basal cells. Epithelial cells were polyhedral and had abundant clear or slightly stained cytoplasm. In the 300‐day foetus the basal layer was thicker, epithelial cells were polyhedral, and there was a flattened 1 cell‐thick layer superficially. In the 335‐day foetus, the epithelial cells were flat and there was a superficial layer of keratinised cells, 1–2 cells thick. In term foals, the gastric epithelium consisted of 10–12 cell layers, with 4–5 thin layers of surface keratin. Epithelial projections (papillae) were either absent or not pronounced. The gastric epithelium became thicker with increasing age, including increased epithelial cell layers, thickening of the keratinised layers, and more pronounced papillae. Desquamation was observed in the keratinised layers of gastric squamous epithelium from foals ≥2 days of age.

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Oral Epidermal Growth Factor Is Trophic for the Stomach in the Neonatal Rat

Cited by 29 publications

References 10 publications

Epidermal Growth Factor Induces Increased Mucosal Thickness of the Small Intestine in Mouse

Epidermal Growth Factor Induces Increased Mucosal Thickness of the Small Intestine in Mouse

Neonatal Gastrointestinal Growth and Function: Are They Regulated by Composition of Feeds?

Age‐related characteristics of gastric squamous epithelial mucosa in foals

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