Oral fluid as a medium for the detection of hepatitis B surface antigen

Hutse, Veronik; Verhaegen, Els; Cock, L. De; Quoilin, Sophie; Vandenberghe, Hans; Horsmans, Yves; Michielsen, P.; Damme, Pierre Van; Vlierberghe, Hans Van; Claeys, F.; Vranckx, R.; Oyen, Herman Van

doi:10.1002/jmv.20413

Cited by 31 publications

(28 citation statements)

References 19 publications

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“…The prevalence of HBV infection is higher among dentists than the general population, especially among those who have surgical specialties [2][3][4] . The occupational risk of HBV infection through contaminated blood in piercing/cutting accidents among dentists varied between 6% and 30% 5,6 .…”

Section: Introductionmentioning

confidence: 99%

Vacinação contra hepatite B e fatores associados entre cirurgiões-dentistas

Ferreira

Guimarães

Pereira

et al. 2012

Rev. bras. epidemiol.

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Objective: We calculated the prevalence and studied the factors associated with hepatitis B vaccination. Reasons for non-vaccination among dentists were investigated. Methods: A cross-sectional study was performed in Montes Claros, Minas Gerais, from 2007 to 2008, to assess hepatitis B vaccination among practicing dental surgeons. Variables were evaluated using a previously tested structured questionnaire. Data underwent descriptive analysis. Non-conditional logistic regression was used to determine the variables associated with hepatitis B vaccination (p < 0.05). Results: There were 333 subjects eligible for the study, 297 (89.2%) participated, and 283 answered the question about vaccination; of these, 258 (91.2%) completed the three-dose schedule vaccination and 25 (8.8%) were not vaccinated or did not complete the vaccination schedule. Lack of information (48%) was stated as the main reason for not being vaccinated. Variables associated with vaccination were: tobacco use (OR = 2.50; IC95% = 1.22-7.13), alcohol consumption (OR = 2.99; IC95% = 1.16-7.74), satisfaction with the profession (OR = 4.62; IC95% = 1.50-8.25) and knowledge on the post-exposure management protocol (OR = 4.28; IC95% = 1.63-9.26). Conclusions:We found a high prevalence of complete vaccination among dentists in Montes Claros. It is higher among professionals who do not use tobacco and alcohol, are more satisfied with their profession and know the protocol after occupational exposure. The study suggests that the lack of information is the main reason for not being vaccinated. As variáveis associadas com relato de vacinação, após ajuste para todas as outras, foram: consumo de tabaco (OR = 2,50; IC95% = 1,22-7,13), consumo de bebidas alcoólicas (OR = 2,99; IC95% = 1,16-7,74), satisfação com a profissão (OR = 4,62; IC95% = 1,50-8,25) e conhecimento sobre protocolo pós-exposição ocupacional (OR = 4,28; IC95% = 1,63-9,26). Conclusões: Verificou-se alta prevalência de vacinação completa, sendo maior entre os não fumantes, os que não consumiam bebidas alcoólicas, os mais satisfeitos com a profissão e os que conheciam um protocolo pós-exposição ocupacional. O estudo sugere que os comportamentos negligentes com a própria saúde se repetem. Há necessidade de campanhas educativas sobre a transmissão da hepatite B, contribuindo para o controle e erradicação dessa grave infecção.Palavras-chaves: Hepatite B. Vacinação. Saúde do trabalhador. Condutas na práti-ca dos dentistas. Doenças transmissíveis. Odontologia.

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Section: Introductionmentioning

confidence: 99%

Vacinação contra hepatite B e fatores associados entre cirurgiões-dentistas

Ferreira

Guimarães

Pereira

et al. 2012

Rev. bras. epidemiol.

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“…As a hospital based population remains a selected population, in 2003, the study population was selected from the general public and a saliva test was used because it is not invasive, not painful, less expensive and does not require trained people to collect the sample. Prior to the prevalence study, tests for detection of hepatitis antibody or antigen in oral fluid were validated [2][3][4]. An additional study objective was to evaluate the feasibility of using saliva tests on samples gathered by regular postal services for prevalence study in order to develop a surveillance tool.…”

Section: Introductionmentioning

confidence: 99%

A population-based prevalence study of hepatitis A, B and C virus using oral fluid in Flanders, Belgium

et al. 2007

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Ten years after the first seroprevalence study performed in Flanders, the aim of this cross sectional study was to follow the evolution of hepatitis A, B and C prevalence. The prevalence of hepatitis A antibodies, hepatitis B surface antigen and hepatitis C antibodies was measured in oral fluid samples collected by postal survey. Using the National Population Register, an incremental sampling plan was developed to obtain a representative sampling of the general population. A total of 24,000 persons were selected and 6,000 persons among them contacted in a first wave. With 1834 participants a response rate of 30.6% was achieved. The prevalence was weighted for age and was 20.2% (95% CI 19.43-21.08) for hepatitis A, 0.66% (95% CI 0.51-0.84) for hepatitis B surface antigen and 0.12% (95% CI 0.09-0.39) for hepatitis C. The prevalence of hepatitis A and C in the Flemish population is lower in 2003 compared with the results of the study performed in 1993. The difference may be due to a real decrease of the diseases but also to differences in the methodology. The prevalence of hepatitis B surface antigen remains stable. Considering the 30% response rate and the high quality of the self-collected samples as reflect of a good participation of the general population, saliva test for prevalence study is a good epidemiological monitoring tool.

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“…Several studies have successfully investigated the use of oral fluid or saliva versus serum for the diagnosis of infectious diseases, including infections with HIV (7), hepatitis A virus (8), dengue virus (9), Helicobacter pylori (10), and malaria (11). In addition, antigens, such as hepatitis B surface antigen (12) and Plasmodium falciparum HRP2 malaria antigen (13), and hormones, such as steroids (14), have been assayed in oral fluid. In the field of toxoplasmosis, some authors have already reported the possibility of detecting anti-T. gondii IgG (15,16), IgM, and IgA (17,18).…”

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confidence: 99%

Use of IgG in Oral Fluid To Monitor Infants with Suspected Congenital Toxoplasmosis

Chapey

Meroni

Kieffer

et al. 2015

Clin. Vaccine Immunol.

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Infants born to mothers who seroconverted for toxoplasmosis during pregnancy are at risk of sequelae. In the case of a negative work-up at birth, congenital infection can be ruled out only by monitoring the disappearance of maternal immunoglobulin G (IgG) transmitted through the placenta, which can be achieved by regular blood sampling during the first year. To alleviate the discomfort of this follow-up, we developed an indirect enzyme-linked immunosorbent assay to detect specific IgG diffusing passively from the blood through the gingival epithelium by collecting oral fluid on microsponges. To assess the feasibility of the test, 212 patients were first enrolled. Levels of specific IgG in oral fluid were significantly higher in seropositive (n ‫؍‬ 195) than in seronegative (n ‫؍‬ 17) patients (mean optical densities, 1.145 ؎ 0.99 versus 0.092 ؎ 0.127; P < 0.0001). In a population of 93 patients <15 months of age born to mothers who displayed toxoplasmic infection during pregnancy, 70 were free of congenital infection and were followed up until their serology turned negative, and 23 were congenitally infected. The same patterns of IgG were observed in the oral fluid and sera in each group. Using a cutoff of 0.04 (optical density value), the sensitivity and specificity of the test were 67.9% and 80.3%, respectively, and the probability of not having a congenital infection when the test on oral fluid was negative was 99%. Although the performance of the test needs to be improved, oral fluid sampling appears to be a promising tool for monitoring infants with suspected congenital toxoplasmosis.

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Oral fluid as a medium for the detection of hepatitis B surface antigen

Cited by 31 publications

References 19 publications

Vacinação contra hepatite B e fatores associados entre cirurgiões-dentistas

Vacinação contra hepatite B e fatores associados entre cirurgiões-dentistas

A population-based prevalence study of hepatitis A, B and C virus using oral fluid in Flanders, Belgium

Use of IgG in Oral Fluid To Monitor Infants with Suspected Congenital Toxoplasmosis

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