One of the factors that influences health behavior is knowledge. Differences in the field of science allow for differences in behavior. Previous research has shown that there are differences in knowledge between students of health and non-health faculties, but there are also studies that show that non-health faculty students behave better than health faculty students. This research aims to find out the differences in knowledge and behavior of genital care between health and non-health faculty students at Universitas Airlangga. The research method is a comparative analytical method with a cross-sectional approach. Data collection was carried out in January–February 2023 at Universitas Airlangga. The total sample was 96 students, 48 students from health faculty, and 48 students from non-health faculty. The sampling technique is purposive sampling. The research instrument is an online questionnaire through Google Forms. The data was analyzed using the Mann-Whitney test. Test results on knowledge data are 0,000 (p value < 0,005) and behavioral data are 0,003 (p value < 0,005). Conclusion: The results of this study show that health faculty students have better knowledge and behavior about genital care than non-health faculty students.