Oral diseases have been a persistent public health problem globally. To assess the practices and oral hygiene status among children aged 4-14 years attended to a dental hospital in Dhaka city, a cross sectional and descriptive study was conducted in Dhaka Dental College and Hospital, Mirpur-14 during the period of January 2019 to June 2019. Among the participants (n=115), 40% of children were between 4-6 years of age, 24.35% between 7-8 years of age, 16.52% between 9 to 10 years and 19.13% between 11-14 years of age. About 51.33% brushed their teeth once daily and 48.67% brushed their teeth twice daily. Majority of the respondents brushed their teeth before breakfast. Most of the (98.26%) children use toothpaste and 1.74% use cinder. It was observed that most of the children didn't use any tooth cleaning material after dinner at night. Most of the children visited dentist whenever there is a pain or troubles with teeth or gums and for extraction of tooth. It was observed that, 15.65% children had good oral hygiene status, fair in 37.39% while, 46.96% had poor oral hygiene. From this study it was found that the children who were brushed their teeth once daily had greater level of poor oral hygiene status than the respondent who were brushed their teeth twice daily. In the present study, gingival bleeding was present in 41.74% and bad breath was present in 43.48% children. Study reveals that 77.8% respondent had poor oral hygiene. For good oral hygiene status preventive measures should be followed like proper brushing techniques and brushing twice daily, rinsing of the mouth properly after meal and regular visit to a dentist.