Oral Human Papillomavirus: a multisite infection

Abstract

Background: The Human Papillomavirus (HPV) has different strategies for persist in the cells. This characteristic has led us to consider the presence of the virus in tissues of the oral cavity that had no clinical signs of infection. The aim of this study was to detect the presence of DNA-HPV at multiple sites of the oral cavity. Material and Methods: A case-control study was designed: Oral Squamous Carcinoma Group (OSCG), healthy n=72 and Control Group (CG), n=72, healthy volunteers paired by sex and age with OSCG. Four samples were taken from OSCG: saliva, biopsy, brush scraping of lesion and contralateral healthy side. In CG a saliva sample and a scratch of the posterior border of tongue were collected. HPV was detected by PCR using Bioneer Accuprep genomic DNA Extraction kit, and consensus primers MY09 and MY11. Chi square test was applied. Results: 432 samples were obtained from 144 individuals. DNA-HPV was detected in 30 (42%) of OSCG subjects and 3 (4%) of CG. Two or more positive samples were obtained in 67% of the OSCG, 67% in saliva and 60% in biopsy; in CG 100% of the individuals were positive in the two samples. Conclusions: HPV is frequently present in oral cavity as a multifocal infection, even without the presence of clinical lesions.

Human papillomavirus and Chlamydia trachomatis in oral and genital mucosa of women with normal and abnormal cervical cytology

“…Although HPV18 genotype is the second more frequent high risk type detected worldwide, in this study, we not detected HPV18 genotype in genital as well as oral mucosa. This is in agreeing with previous studies in oral mucosa in Argentina [ 3 , 11 , 23 , 29 ]. Reference to genital mucosa, a Brazilian study not detected HPV18 genotype in normal mucosa [ 1 , 25 ].…”

“…Reference to genital mucosa, a Brazilian study not detected HPV18 genotype in normal mucosa [ 1 , 25 ]. The more frequent HPV genotype detected was type 16; this result is in concordance with others studies in oral as well as genital mucosa [ 3 , 5 , 11 , 18 , 23 , 25 , 28 , 29 ]. The coinfection detected in this work was high risk genotypes 16 and 31 in genital mucosa.…”

Human Papillomavirus and Chlamydia trachomatis in oral and genital mucosa of women with normal and abnormal cervical cytology

“…Although HPV18 genotype is the second more frequent high risk type detected worldwide, in this study, we not detected HPV18 genotype in genital as well as oral mucosa. This is in agreeing with previous studies in oral mucosa in Argentina [3,9,22,27]. Reference to genital mucosa, the Brazilian study not detected HPV18 genotype in normal mucosa [1,24].…”

“…Reference to genital mucosa, the Brazilian study not detected HPV18 genotype in normal mucosa [1,24]. The more frequent HPV genotype detected was type 16; this result is in concordance with others studies in oral as well as genital mucosa [3,5,9,14,22,24,26,27]. The coinfection detected in this work was high risk genotypes 16 and 31 in genital mucosa.…”

Biomarkers of human papillomavirus (HPV)‐driven head and neck cancer in Latin America and Europe study: Study design and HPV DNA/p16INK4a status

“…35,36 Among samples from Argentina, we detected HPV DNA in 25.0% (31/124) of the OCC samples analyzed, which contrasts to two other studies in which about 40% of OCC samples were found to harbor viral DNA. 37,38 Divergence in HPV DNA rates observed among studies conducted in different countries of Latin America and even within the same country could be attributed not only to differences in the methodological approaches used for viral DNA detection, but also to the characteristics of the individuals under study, including sexual practices, economic and social statuses. We are in the process of analyzing several of these variables, but unfortunately, the absence of sexual behavior information of the individuals evaluated by us hampers any additional analysis.…”