Oral Hypoglycemic Activity of Culinary-Medicinal Mushrooms Pleurotus ostreatus and P. cystidiosus (Higher Basidiomycetes) in Normal and Alloxan-Induced Diabetic Wistar Rats

Abstract

This study investigates the oral hypoglycemic activity of Pleurotus ostreatus (P.o.) and P. cystidiosus (P.c.) mushrooms on normal and alloxan-induced diabetic Wistar rats. Different doses (250, 500, 750, 1000, and 1250 mg/kg/body weight) of suspensions of freeze-dried and powdered (SFDP) P.o. and P.c. were administered to normal rats, and postprandial serum glucose levels were measured. Optimal time of activity was investigated using the dose 500 mg/kg. Hypoglycemic effect of a single dose of SFDP P.o. and P.c. (500 mg/kg) were investigated using diabetic male and female rats at different stages of estrous cycle and compared with metformin and glibenclamide. Chronic hypoglycemic activity of SFDP P.o. and P.c. (500 mg/kg) was studied using serum glucose levels and glycosylated hemoglobin levels. Maximally effective dose of SFDP P.o. and P.c. was 500 mg/kg. The highest reduction in the serum glucose level was observed 120 minutes after administration of mushrooms. A single dose of P.o. and P.c. significantly (P < 0.05) reduced the serum glucose levels of male diabetic rats. The hypoglycemic activity in female rats was highest in proestrous stage. The hypoglycemic effect of P.o. and P.c. is comparable with metformin and glibenclamide. Daily single administrations of P.o. and P.c. to diabetic rats exert apparent control on the homeostasis of blood glucose. SFDP P.o. and P.c. possessed marked and significant oral hypoglycemic activity. This study suggests the consumption of P.o. and P.c. mushrooms might bring health benefits to mankind as it shows hypoglycemic activity in rats.

Hypoglycaemic Activity of CulinaryPleurotus ostreatusandP.cystidiosusMushrooms in Healthy Volunteers and Type 2 Diabetic Patients on Diet Control and the Possible Mechanisms of Action

“…The significant fall in postprandial serum glucose levels in normal and alloxan‐induced diabetic rats in the present study (Fig. A) are on par with our previous findings (Jayasuriya et al ., ). The mushrooms had enhanced the absorption of glucose from the intestines as evident by the lower level of glucose in the intestinal contents of the mushroom groups.…”

“…The P.o and P.c commonly known as American oyster and abalone, respectively, were shown to possess hypocholesterolaemic, antioxidant, antitumour, hepatoprotective, hypotensive, antinociceptive and antifungal activity (Abeytunga, 2011). In our previous studies, we demonstrated the promising acute and chronic oral hypoglycaemic potential of P.o and P.c in both normal and alloxan-induced diabetic animals as well as the antiinflammatory activity of P.o (Jayasuriya et al, 2012a;Jayasuriya et al, 2012b).…”

“…c at a dose of 500 mg/kg, respectively. In our previous study, the dose of 500 mg/kg was identified as the maximally effective dose (Jayasuriya et al ., ). Test 5 and 6 groups were fed with distilled water.…”

“…c in both normal and alloxan‐induced diabetic animals as well as the anti‐inflammatory activity of P . o (Jayasuriya et al ., ; Jayasuriya et al ., ).…”

“…o and P . c in rats (Jayasuriya et al ., ) has resulted in studies to establish the effect in humans. Hence, the purpose of the present study was to investigate the oral hypoglycaemic potential of P .…”