2022
DOI: 10.1007/s00284-022-02866-w
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Oral Immunization of Mice with Cell Extracts from Recombinant Lactococcus lactis Expressing SARS-CoV-2 Spike Protein

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Cited by 8 publications
(6 citation statements)
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“…Mucosal immunity significantly contributes to the defense against viral pathogens by providing a frontline barrier and an effective immune response. Immunization via oral vaccine administration, incorporating specific antigens, stimulates the synthesis of secretory immunoglobulin A (IgA) on mucosal surfaces ( 33 , 34 ). Studies showed that oral immunization of mice with lactic acid bacteria expressing the SARS-CoV-2 spike (S) protein receptor-binding domain (RBD) S1 subunit increased the fecal IgA levels 1.4-fold higher in the immunized mice than controls after two boosting doses on day 22 ( 33 ).…”
Section: Discussionmentioning
confidence: 99%
“…Mucosal immunity significantly contributes to the defense against viral pathogens by providing a frontline barrier and an effective immune response. Immunization via oral vaccine administration, incorporating specific antigens, stimulates the synthesis of secretory immunoglobulin A (IgA) on mucosal surfaces ( 33 , 34 ). Studies showed that oral immunization of mice with lactic acid bacteria expressing the SARS-CoV-2 spike (S) protein receptor-binding domain (RBD) S1 subunit increased the fecal IgA levels 1.4-fold higher in the immunized mice than controls after two boosting doses on day 22 ( 33 ).…”
Section: Discussionmentioning
confidence: 99%
“…In these contexts, gmLAB expressing therapeutic proteins have been evaluated as next-generation probiotics and microbial therapeutics [26][27][28]. For example, the effectiveness of oral administration of an L. lactis strain expressing the SARS-CoV-2 spike protein has been studied for COVID-19 vaccination [29]. Therefore, in the present study, we constructed gmLAB that express CC12.1scFv and assessed its immunoreactivity.…”
Section: Discussionmentioning
confidence: 99%
“…In particular, L. lactis has been engineered as a live vehicle for the delivery of DNA vaccines and production of therapeutic biomolecules ( Tavares et al, 2020 ). In bacterial cell extracts study, cell extracts from recombinant L. lactis expressing SARS-CoV-2 spike protein were used to oral immunize mice, and antigen-specific antibodies were produced from immunized mice ( Xuan et al, 2022 ). L. lactis IL1403 is a representative laboratory strain that can be used to produce recombinant heterologous proteins ( Tavares et al, 2020 ), and there is no evidence to date that metabolites of L. lactis IL1403 are toxic to experimental animals.…”
Section: Discussionmentioning
confidence: 99%