Oral immunization with recombinant Lactobacillus casei displayed AHA1-CK6 and VP2 induces protection against infectious pancreatic necrosis in rainbow trout (Oncorhynchus mykiss)

Chen, Yaping; Hua, Xiaojing; Ren, Xuanyu; Duan, Kexin; Gao, Shuai; Sun, Jinhui; Feng, Ying; Zhou, Ying; Guan, Xin; Li, Dechuan; Wang, Na; Li, Jiahui; Yang, Jiawei; Xia, Dong; Shi, Wen

doi:10.1016/j.fsi.2020.03.001

Cited by 22 publications

(18 citation statements)

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“…There, the immune cells could stimulate other cells either by endogenous release of interferon or by the release of interferon produced by MT006, in a mechanism similar to that observed when dendritic cells interact with commensal bacteria in the mammalian intestine and transport them to the lymph nodes (89). This mechanism could explain the immunizing properties of orally administered recombinant Lactic Acid Bacteria that express epitopes from microbial pathogens of fish (33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44). The dosages used in these experiments are between 10 7 to 2x10 8 CFU per fish gram, around 30 to 600 times the used dosage of MT006.…”

Section: Discussionmentioning

confidence: 90%

“…The oral administration of these improved probiotics produces systemic effects, conferring protection against virus (29), bacterial pathogens (30,31), and parasites (32). In salmonids, oral administration of Lactobacillus casei species expressing IPNV epitopes has shown to confer protection against the virus (33)(34)(35)(36)(37), while Lactococcus lactis strains have been used to orally immunize against Hirame novirhabdovirus in flounder (38), Carp spring viremia in common carps (39), the protozoa Ichthyophthirius multifiliis in Goldfish (40), and against the viral hemorrhagic septicemia in rainbow trout (41). Oral administration of recombinant Lactobacillus casei expressing epitopes of Aeromona veronii confers protection against this pathogen in Cyprinus carpio (42,43), and Carassius carassius (44), while Lactobacillus plantarum expressing G protein of spring viremia of carp virus (SVCV) (45) and the ORF81 protein of koi herpesvirus (KHV) has shown confers protection against both viruses with high titers of IgM after its oral administration to Cyprinus carpio (46).…”

Section: Introductionmentioning

confidence: 99%

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Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

et al. 2021

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In salmon farming, viruses are responsible for outbreaks that produce significant economic losses for which there is a lack of control tools other than vaccines. Type I interferon has been successfully used for treating some chronic viral infections in humans. However, its application in salmonids depends on the proper design of a vehicle that allows its massive administration, ideally orally. In mammals, administration of recombinant probiotics capable of expressing cytokines has shown local and systemic therapeutic effects. In this work, we evaluate the use of Lactococcus lactis as a type I Interferon expression system in Atlantic salmon, and we analyze its ability to stimulate the antiviral immune response against IPNV, in vivo and in vitro. The interferon expressed in L. lactis, even though it was located mainly in the bacterial cytoplasm, was functional, stimulating Mx and PKR expression in CHSE-214 cells, and reducing the IPNV viral load in SHK-1 cells. In vivo, the oral administration of this L. lactis producer of Interferon I increases Mx and PKR expression, mainly in the spleen, and to a lesser extent, in the head kidney. The oral administration of this strain also reduces the IPNV viral load in Atlantic salmon specimens challenged with this pathogen. Our results show that oral administration of L. lactis producing Interferon I induces systemic effects in Atlantic salmon, allowing to stimulate the antiviral immune response. This probiotic could have effects against a wide variety of viruses that infect Atlantic salmon and also be effective in other salmonids due to the high identity among their type I interferons.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

et al. 2021

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“…In 1997, Maeda et al performed a natural infection of the yellow jack (Carangoides bartholomaei) larvae with Sima-aji Neuro Necrosis Virus (SJNNV), reporting that the bacterial strain Pseudoalteromonas undina VKM-124 showed an inhibitory activity towards SJNNV (250), with recombinant L. lactis NZ3900 expressing the G gene of viral hemorrhagic septicemia virus (VHSV) resulted in a significant reduction of viral loads and decreased fish mortality after viral challenge (255). Increased resistance to IPNV was also detected in rainbow trout orally immunized with recombinant L. casei expressing the viral antigens (264). Similar results were observed in common carp orally immunized with recombinant L. plantarum expressing the G protein of spring viremia of carp virus (SVCV).…”

Section: Probiotics Providing Resistance To Viral Pathogensmentioning

confidence: 99%

Mechanisms Used by Probiotics to Confer Pathogen Resistance to Teleost Fish

Simón¹,

Docando²,

Nuñez-Ortiz³

et al. 2021

Front. Immunol.

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Probiotics have been defined as live microorganisms that when administered in adequate amounts confer health benefits to the host. The use of probiotics in aquaculture is an attractive bio-friendly method to decrease the impact of infectious diseases, but is still not an extended practice. Although many studies have investigated the systemic and mucosal immunological effects of probiotics, not all of them have established whether they were actually capable of increasing resistance to different types of pathogens, being this the outmost desired goal. In this sense, in the current paper, we have summarized those experiments in which probiotics were shown to provide increased resistance against bacterial, viral or parasitic pathogens. Additionally, we have reviewed what is known for fish probiotics regarding the mechanisms through which they exert positive effects on pathogen resistance, including direct actions on the pathogen, as well as positive effects on the host.

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“…The antigens in a vaccine may be broken down and inactivated in the gastrointestinal tract before reaching the intestinal mucosa and activating immune cells ( 144 ). The availability of antigens will thus be lowered, and a low antigen dose will induce regulatory T-cell-mediated immune tolerance ( 92 ).…”

Section: Orally Administered Immunoprophylactics and The Implication In The Gut Immune Systemmentioning

confidence: 99%

“…Orally vaccinated fish exhibited induced expression of β-defensin in the intestine, higher anti-VP2 IgT titer in the skin mucus and lower viral load in the liver and pancreas compared to the control group ( 162 ). To further boost the effects of this oral vaccine, a genetically engineered Lactobacillus casei was constructed to present the Aeromonas hydrophila adhesion (AHA1) - CK6 - VP2 fusion protein ( 144 ). Recombinant AHA1 protein was illustrated to adhere to epithelial cells possibly due to its strong hydrophobicity that can bind to the cell surface receptor via covalent bonds ( 175 ).…”

Section: Orally Administered Immunoprophylactics and The Implication In The Gut Immune Systemmentioning

confidence: 99%

Gut Immune System and the Implications of Oral-Administered Immunoprophylaxis in Finfish Aquaculture

et al. 2021

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The gastrointestinal immune system plays an important role in immune homeostasis regulation. It regulates the symbiotic host-microbiome interactions by training and developing the host’s innate and adaptive immunity. This interaction plays a vital role in host defence mechanisms and at the same time, balancing the endogenous perturbations of the host immune homeostasis. The fish gastrointestinal immune system is armed with intricate diffused gut-associated lymphoid tissues (GALTs) that establish tolerance toward the enormous commensal gut microbiome while preserving immune responses against the intrusion of enteric pathogens. A comprehensive understanding of the intestinal immune system is a prerequisite for developing an oral vaccine and immunostimulants in aquaculture, particularly in cultured fish species. In this review, we outline the remarkable features of gut immunity and the essential components of gut-associated lymphoid tissue. The mechanistic principles underlying the antigen absorption and uptake through the intestinal epithelial, and the subsequent immune activation through a series of molecular events are reviewed. The emphasis is on the significance of gut immunity in oral administration of immunoprophylactics, and the different potential adjuvants that circumvent intestinal immune tolerance. Comprehension of the intestinal immune system is pivotal for developing effective fish vaccines that can be delivered orally, which is less labour-intensive and could improve fish health and facilitate disease management in the aquaculture industry.

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Oral immunization with recombinant Lactobacillus casei displayed AHA1-CK6 and VP2 induces protection against infectious pancreatic necrosis in rainbow trout (Oncorhynchus mykiss)

Cited by 22 publications

References 46 publications

Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

Mechanisms Used by Probiotics to Confer Pathogen Resistance to Teleost Fish

Gut Immune System and the Implications of Oral-Administered Immunoprophylaxis in Finfish Aquaculture

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