Oral Infection and Transmission of La Crosse Virus by an Enzootic Strain of Aedes Triseriatus Feeding on Chipmunks with a Range of Viremia Levels

La, Patrican; Gr, DeFoliart; Tm, Yuill

doi:10.4269/ajtmh.1985.34.992

Cited by 11 publications

(6 citation statements)

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“…taeniorhynchus infected intrathoracically had a significantly higher mean saliva titer than those exposed via an artificial blood meal or viremic animal. Although mosquitoes can be less susceptible to infection using artificial blood meals compared with natural infection, [6][7][8]29 our study found no difference in the dissemination rates observed after oral infection using the 2 methods, although there was a a 10-fold difference in the infectious dose.…”

Section: Discussioncontrasting

confidence: 56%

“…Oral infection via artificial blood meals or viremic animals more closely resembles natural exposure. Several studies have demonstrated that mosquitoes are less susceptible to infection by artificial than by natural blood meals [6][7][8] due to the concentration of virus adjacent to the midgut epithelium that follows natural but not artificial blood meals. 9 In contrast, one study of Aedes aegypti mosquitoes infected with Semliki Forest virus found no difference in virus titers in infected mosquitoes after artificial versus natural blood meals except during the first 24 hours, and transmission did not differ significantly.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Methods to Assess Transmission Potential of Venezuelan Equine Encephalitis Virus by Mosquitoes and Estimation of Mosquito Saliva Titers

Smith

Carrara

Aguilar

et al. 2005

The American Journal of Tropical Medicine and Hygiene

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Determining the dose of an arbovirus transmitted by a mosquito is important to design transmission and pathogenesis studies simulating natural infection. Several different artificial infection and transmission methods used to assess vector competence and to estimate the dose injected during mosquito feeding have not been fully evaluated to determine whether they accurately reflect natural transmission. Additionally, it is not known whether different mosquito vectors transmit similar amounts of a given virus. Therefore, we compared three traditional artificial transmission methods using Venezuelan equine encephalitis virus (VEEV) and Aedes albopictus and Ochlerotatus taeniorhynchus mosquitoes. Both the mosquito species and the infection route used affected the amount of virus detected in the saliva after a 10-day extrinsic incubation period. Median titers of virus detected in saliva of Ae. albopictus and Oc. taeniorhynchus mosquitoes ranged from 0.2 to 1.1 log(10) (mean 0.7-1.4 log(10)) and 0.2 to 3.2 log(10) (mean 1.0-3.6 log(10)) plaque-forming units, respectively. The results of this study will aid in the design of transmission and pathogenesis studies involving arboviruses.

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Evaluation of Methods to Assess Transmission Potential of Venezuelan Equine Encephalitis Virus by Mosquitoes and Estimation of Mosquito Saliva Titers

Smith

Carrara

Aguilar

et al. 2005

The American Journal of Tropical Medicine and Hygiene

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“…We found that the difference in infectivity of MAYV was approximately five times greater when mosquitoes were fed on a viremic animal rather than an artificial blood meal, a difference that has been recognized to various degrees in previous studies. [41][42][43][44] The threshold of oral infection of Ae. aegypti with MAYV is estimated to be between 5.0 and 5.5 log 10 PFU/mL, with 0-24% of mosquitoes becoming infected at artificial blood meal doses ranging from 5.1 to 5.6 log 10 PFU/mL.…”

Section: Discussionmentioning

confidence: 99%

Experimental Transmission of Mayaro Virus by Aedes aegypti

Long

Ziegler

Thangamani³

et al. 2011

The American Society of Tropical Medicine and Hygiene

202

209

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Abstract. Outbreaks of Mayaro fever have been associated with a sylvatic cycle of Mayaro virus (MAYV) transmission in South America. To evaluate the potential for a common urban mosquito to transmit MAYV, laboratory vector competence studies were performed with Aedes aegypti from Iquitos, Peru. Oral infection in Ae. aegypti ranged from 0% (0/31) to 84% (31/37), with blood meal virus titers between 3.4 log 10 and 7.3 log 10 plaque-forming units (PFU)/mL. Transmission of MAYV by 70% (21/30) of infected mosquitoes was shown by saliva collection and exposure to suckling mice. Amount of viral RNA in febrile humans, determined by real-time polymerase chain reaction, ranged from 2.7 to 5.3 log 10 PFU equivalents/mL. Oral susceptibility of Ae. aegypti to MAYV at titers encountered in viremic humans may limit opportunities to initiate an urban cycle; however, transmission of MAYV by Ae. aegypti shows the vector competence of this species and suggests potential for urban transmission.

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“…Other studies indicated that larval stress, primarily caused by food limitation within habi tats, not only produced small adults, but adversely affected larval survival, developmental rates, and adult fitness (62,70,73,110). For example, in some laboratory studies, smaller females (which reflects reduced larval food availability) were more competent vectors for arboviruses than larger females (60,68,115). An understanding of the spatial and temporal distribution of the dietary resources available to larval mosquitoes in their natural habitats could clarify the relationships among food availability, vector competence, and" mosquito fitness.…”

mentioning

confidence: 97%

Feeding Behavior, Natural Food, and Nutritional Relationships of Larval Mosquitoes

Merritt¹,

Dadd²,

Walker³

1992

Annu. Rev. Entomol.

579

534

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Oral Infection and Transmission of La Crosse Virus by an Enzootic Strain of Aedes Triseriatus Feeding on Chipmunks with a Range of Viremia Levels

Cited by 11 publications

References 0 publications

Evaluation of Methods to Assess Transmission Potential of Venezuelan Equine Encephalitis Virus by Mosquitoes and Estimation of Mosquito Saliva Titers

Evaluation of Methods to Assess Transmission Potential of Venezuelan Equine Encephalitis Virus by Mosquitoes and Estimation of Mosquito Saliva Titers

Experimental Transmission of Mayaro Virus by Aedes aegypti

Feeding Behavior, Natural Food, and Nutritional Relationships of Larval Mosquitoes

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