Oral Ingestion of Bacterially Expressed dsRNA Can Silence Genes and Cause Mortality in a Highly Invasive, Tree-Killing Pest, the Emerald Ash Borer

Leelesh, Ramya Shanivarsanthe; Rieske, Lynne K.

doi:10.3390/insects11070440

Cited by 27 publications

(20 citation statements)

References 35 publications

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“…The authors observed 30, 35, and 78% larval mortality associated with 1, 6, and 10 μg dsRNA/μl treatments, respectively. More recently, Leelesh and Rieske (2020) demonstrated 47–69% mortality (depending on target gene) in A. planipennis larvae fed bacterially expressed dsRNA (at approximately 100 bacterial cells/nl).…”

Section: Observed Rnai Sensitivities In Coleopteramentioning

confidence: 99%

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Willow

Veromann

2021

Front. Plant Sci.

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Many herbivorous beetles (Order Coleoptera) contribute to serious losses in crop yields and forest trees, and plant biotechnology solutions are being developed with the hope of limiting these losses. Due to the unprecedented target-specificity of double-stranded RNA (dsRNA), and its utility in inducing RNA interference (RNAi) when consumed by target pest species, dsRNA-based plant biotechnology approaches represent the cutting edge of current pesticide research and development. We review dietary RNAi studies in coleopterans and discuss prospects and future directions regarding RNAi-based management of coleopteran plant pests. Herein, we also provide a balanced overview of existing studies in order to provide an accurate re-assessment of dietary RNAi sensitivity in coleopterans, despite the limitations to the existing body of scientific literature. We further discuss impediments to our understanding of RNAi sensitivity in this important insect order and identify critical future directions for research in this area, with an emphasis on using plant biotechnology approaches.

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Section: Observed Rnai Sensitivities In Coleopteramentioning

confidence: 99%

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Willow

Veromann

2021

Front. Plant Sci.

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“…Further, studies also suggested that extracts of bacterially expressed dsRNA can be applied in pest control, particularly when the use of live bacteria is limited or the RNAi efficiency of use of bacteria expressing dsRNA is dissatisfied ( Leelesh and Rieske 2020 ). And recent studies also showed that nanomaterial-wrapped dsRNA has higher stability and better cellular uptake, providing a potential RNAi-based strategy for pest control ( Sun et al 2020b ).…”

Section: Discussionmentioning

confidence: 99%

Interference Efficiency and Effects of Bacterium-mediated RNAi in the Fall Armyworm (Lepidoptera: Noctuidae)

Wan

Shi

et al. 2021

Journal of Insect Science

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RNAi is an effective tool for gene function analysis and a promising strategy to provide environmentally friendly control approaches for pathogens and pests. Recent studies support the utility of bacterium-mediated RNAi as a cost-effective method for gene function study and a suitable externally applied delivery mechanism for pest control. Here, we developed a bacterium-mediated RNAi system in Spodoptera frugiperda based on four target genes, specifically, Chitinase (Sf-CHI), Chitin synthase B (Sf-CHSB), Sugar transporter SWEET1 (Sf-ST), and Hemolin (Sf-HEM). RNAi conducted by feeding larvae with bacteria expressing dsRNAs of target genes or injecting pupae and adults with bacterially synthesized dsRNA induced silencing of target genes and resulted in significant negative effects on growth and survival of S. frugiperda. However, RNAi efficiency and effects were variable among different target genes and dsRNA delivery methods. Injection of pupae with dsCHI and dsCHSB induced a significant increase in wing malformation in adults, suggesting that precise regulation of chitin digestion and synthesis is crucial during wing formation. Injection of female moths with dsHEM resulted in lower mating, fecundity, and egg hatching, signifying a critical role of Sf-HEM in the process of egg production and/or embryo development. Our collective results demonstrate that bacterium-mediated RNAi presents an alternative technique for gene function study in S. frugiperda and a potentially effective strategy for control of this pest, and that Sf-CHI, Sf-CHSB, Sf-ST, and Sf-HEM encoding genes can be potent targets.

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“…The mRNA level of ace1 was reduced by 86.9% at the 5 days after the ingestion of E. coli resuspension containing 20 ng/μl dsRNA. Compared with studies in insects, the RNAi efficiency achieved here was also acceptable (Guo et al ., 2015; Al Baki et al ., 2020; Bento et al ., 2020; Leelesh and Rieske, 2020).…”

Section: Discussionmentioning

confidence: 99%

RNA interference in Pardosa pseudoannulata, an important predatory enemy against several insect pests, through ingestion of dsRNA‐expressing Escherichia coli

Hou

Zhou

Chen

et al. 2021

Insect Molecular Biology

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RNA interference is an important technology for gene functional research in many organisms. The pond wolf spider (Pardosa pseudoannulata) is an important natural enemy of rice field pests. To facilitate large‐scale gene functional research in this spider species and others, we developed an RNA interference (RNAi) method via ingestion of bacteria expressing dsRNA. The dsRNA targeting a cytochrome P450 monooxygenase (cyp41g2) was expressed in Escherichia coli HT115 (DE3). And then the bacterial suspension was fed to 14–20 days old spiderlings. The mRNA abundance of the target gene was significantly reduced after 3‐day's ingestion of bacteria expressing dsRNA, and between day 5 and 7, RNAi efficiency remained stable. Thus, we selected 5 days as the optimum interference time. Furthermore, the bacteria resuspension containing 20 ng/μl dsRNA was selected as the optimum concentration. To evaluate the applicability of this method, three other genes with different tissue expression pattern were also selected as targets. And the mRNA abundance of all the four target genes was significantly reduced with RNAi efficiency between 66.0% and up to 86.9%. The results demonstrated that the oral delivery of bacteria expressing dsRNA would be an effective RNAi method for the gene functional study in P. pseudoannulata.

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Oral Ingestion of Bacterially Expressed dsRNA Can Silence Genes and Cause Mortality in a Highly Invasive, Tree-Killing Pest, the Emerald Ash Borer

Cited by 27 publications

References 35 publications

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Interference Efficiency and Effects of Bacterium-mediated RNAi in the Fall Armyworm (Lepidoptera: Noctuidae)

RNA interference in Pardosa pseudoannulata, an important predatory enemy against several insect pests, through ingestion of dsRNA‐expressing Escherichia coli

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