Oral JS-38, a metabolite from Xenorhabdus sp., has both anti-tumor activity and the ability to elevate peripheral neutrophils

Liu, Min-Yu; Lin, Xiao; Chen, Geng-Hui; Wang, Yongxiang; Xiong, Wei-xia; Li, Fēi; Liu, Ying; Huang, Xiaoling; Deng, Yi-Fang; Zhang, Zhen; Sun, Haiyan; Quan-hai, Liu; Yin, Ming

doi:10.1016/s1875-5364(14)60117-5

Cited by 1 publication

(2 citation statements)

References 25 publications

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“…The spleen mainly generates the mature lymphocytes, including the Th type lymphocytes (CD 3 + CD 4 + ) and Tc type lymphocytes (CD 3 + CD 8 + ), and the ratio of CD 3 + CD 4 + /CD 3 + CD 8 + is an indicator of immune dysfunction. In consistent with previous researches, 14,15,29,30 we also found that CTX caused spleen atrophy, perturbed the balance of CD 3 + CD 4 + /CD 3 + CD 8 + , and suppressed lymphocyte proliferation response in normal or LLC-bearing mice, but LP treatment remarkably reversed these changes caused by CTX.…”

Section: Discussionsupporting

confidence: 92%

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Immune regulation effect of lienal polypeptides extract in Lewis lung carcinoma-bearing mice treated with cyclophosphamide

Deng

Ouyang

et al. 2017

Exp Biol Med (Maywood)

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Polypeptides extracted from animal immune organs have been proved to exert immunomodulatory activities in previous reports. However, relative experimental data regarding the influence of a polypeptide mixture extracted from healthy calf spleen (lienal polypeptide [LP]) on the immune function in tumor therapy are limited, and the components in LP remain unclear. In the present study, the immune regulatory effect of LP was investigated in normal mice and Lewis lung carcinoma (LLC)-bearing mice treated with cyclophosphamide (CTX). The components of LP were identified by liquid chromatography-electrospray ionization-coupled with tandem mass spectrometry (LC-MS/MS) analysis and bioinformatic analysis. In LLC-bearing mice, LP showed a synergic antitumor effect with CTX, whereas LP alone did not present direct antitumor activity. Further, LP was found to enhance immune organ indexes, splenocyte number, and T lymphocyte subsets in normal mice and LLC-bearing mice treated with CTX. The decline of white blood cell and platelet counts, splenocyte proliferation activity, and peritoneal macrophage phagocytic function caused by CTX were also significantly suppressed by LP treatment in LLC-bearing mice. Notably, LP treatment significantly decreased the expression of phagocytosis-related proteins including CD47/signal regulatory protein α/Src homology phosphatase-1 in the tumor tissue of LLC-bearing mice treated with CTX. LC-MS/MS-based peptidomics unraveled the main polypeptides in LP with a length from 8 to 25 amino acids. Bioinformatics analysis further confirmed the possibility of LP to regulate immunity, especially in phagocytosis-related pathway. Our above findings indicated that LP can relieve the immunosuppression induced by chemotherapy and is a beneficial supplement in cancer therapy. Impact statement The immunomodulatory activities of polypeptides extracted from animal immune organs have incurred people's interests since a long time ago. In this study, we investigated the immune regulation effects of a polypeptide mixture extracted from health calf spleen (lienal polypeptide [LP]) in Lewis lung carcinoma-bearing mice treated with cyclophosphamide (CTX). Liquid chromatography-electrospray ionization-coupled with tandem mass spectrometry-based peptidomics and bioinformatics analysis unraveled the main polypeptides in LP and further confirmed that LP is mainly associated with immune regulating pathway, especially in tumor cell phagocytosis-related pathway. Our study for the first time revealed that polypeptides from spleen can relieve the immunosuppression induced by CTX and is a beneficial supplement in cancer therapy.

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Section: Discussionsupporting

confidence: 92%

“…The dose of CTX (20 mg/kg) was chosen according to previous reports. 13–16 On the sixth day after subcutaneous inoculation of LLC cells, CTX, LP, and TP5 were administered, respectively, to mice once daily by intraperitoneal injection lasted for 15 days. Body weight of each mouse was monitored every day.…”

Section: Methodsmentioning

confidence: 99%

Immune regulation effect of lienal polypeptides extract in Lewis lung carcinoma-bearing mice treated with cyclophosphamide

Deng

Ouyang

et al. 2017

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

show abstract

Oral JS-38, a metabolite from Xenorhabdus sp., has both anti-tumor activity and the ability to elevate peripheral neutrophils

Cited by 1 publication

References 25 publications

Immune regulation effect of lienal polypeptides extract in Lewis lung carcinoma-bearing mice treated with cyclophosphamide

Immune regulation effect of lienal polypeptides extract in Lewis lung carcinoma-bearing mice treated with cyclophosphamide

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