Oral Kohki Tea and its protective effect against in vitro ischemic damage to the bladder

Levin, Robert M.; Leggett, Robert E.; Whitbeck, Catherine; Murakami, Toshiyuki; Kambara, Toshimitsu

doi:10.1002/nau.20033

Cited by 12 publications

(5 citation statements)

References 34 publications

(36 reference statements)

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“…The probable role of the oxidative stress on the physiopathology of the bladder dysfunction remains controversial. Previous studies showed that an ischemic period in the bladder, which is subjected to an acute distention, was followed by a reperfusion period resulting in free radical production and these studies speculated about a probable association of the reperfusion period with the chronic infravesical obstruction 17 , 18 . In these reports, MDA, which is the end product of the lipid peroxidation caused by free radicals, was used as a marker of cell membrane damage and disruption.…”

Section: Discussionmentioning

confidence: 99%

Urethral monopolar cauterization: alternative infravesical obstruction model in male rats

Akan

Tavukçu

Söğüt

et al. 2022

Rev. Assoc. Med. Bras.

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OBJECTIVE:We aimed to determine which method gives the most consistent results between urethral monopolar cauterization and standard urethral partial ligation methods for the urethral obstruction model. METHODS: Thirty male rats were randomly divided into control, partial ligation, and monopolar cauterization groups. Six weeks after experimental procedures, the experimental groups were evaluated cystometrically, biochemically, and histologically. RESULTS: According to the cystometric results, bladder capacity, baseline bladder pressure, and compliance data of the monopolar cauterization group were higher than those of the partial ligation and monopolar cauterization groups (p<0.05 and p<0.01, respectively). As a biochemical evaluation, malondialdehyde levels in bladder tissues of group control were higher than partial ligation and monopolar cauterization groups (p<0.05 and p<0.01, respectively). The collagen type I level of the control group was higher than the partial ligation and monopolar cauterization groups (p<0.01 and p<0.05, respectively). Collagen type III levels of the monopolar cauterization group were higher than those of the control group (p<0.01), but the Collagen type I/Collagen type III and transforming growth factor-β levels of the monopolar cauterization group were significantly lower than those of the control group (p<0.001). As a histological evaluation (hematoxylin and eosin), fibrosis in the lamina propria was more prominent in the monopolar cauterization group than in the control group (p<0.05). In addition, the muscular thickness was higher in the monopolar cauterization group compared with control and partial ligation groups (p<0.001 and p<0.01, respectively). CONCLUSION: The needle-tipped monopolar cauterization of the posterior urethra may be the method of choice for creating a chronic infravesical obstruction model of infravesical obstruction in male rats.

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Section: Discussionmentioning

confidence: 99%

Urethral monopolar cauterization: alternative infravesical obstruction model in male rats

Akan

Tavukçu

Söğüt

et al. 2022

Rev. Assoc. Med. Bras.

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“…Zhao et al were the first investigators to suggest that a bladder submitted to acute distension would initially present a period of ischemia, followed by a period of reperfusion, which would lead to the generation of free radicals and tissue lesions (10). More recently, a study developed by Levin et al demonstrated a possible relationship between a period of ischemia followed by reperfusion and the etiology of the bladder dysfunction subsequent to a period of chronic infravesical obstruction (7). These authors formulated the hypothesis that a state of oxidative stress would be present in a situation of chronic obstruction and that this would lead to the formation of metabolic end products that would cause damage to the phospholipids of the lipid membrane of the muscle cells.…”

Section: Discussionmentioning

confidence: 99%

“…The tubes were centrifuged at 1000 x G for 5 min and the top layer was measured fluorometrically at 532 nm. Total quantification of proteins was done by means of the Micro BCA protein analyzer, and the results were expressed as nmoL MDA/mg protein (7). The MDA levels were described in relation to the total protein levels.…”

Section: Malondialdehyde (Mda)mentioning

confidence: 99%

Influence of oxidative stress on inducing micturition dysfunction following chronic infravesical obstruction and the protective role of an antioxidant diet - association of in vivo and in vitro studies in rats

Bisogni¹,

Ferreira

Neto

et al. 2012

Int. braz j urol.

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ARTIcLE INFO _________________________________________________________ ___________________Objective: This study was developed to determine whether the generation of free radicals, induced by ischemia followed by reperfusion in a model of chronic intravesical obstruction in rats, would lead to damage in the detrusor. It also investigates the possible protective action of the flavonoid galangin on the tissue lesion induced by lipid peroxidation. Materials and Methods: Twenty-one male rats were divided into three groups of seven animals each. Group A was subjected to a sham procedure; group B to partial obstruction of the bladder neck; and group C to partial obstruction of the bladder neck, but also received a diet rich in the flavonoid galangin. All the animals were subjected to urodynamic evaluation and then sacrificed. The bladders were sent for enzymatic tests. Results:The urodynamic showed that group B developed significantly greater numbers of involuntary contractions of the detrusor, greater post-micturition residue and lower compliance. The group A presented TEAC levels greater than to the group B. Comparative analysis of group A, B and C demonstrated significantly greater malondialdehyde levels in group B in relation to groups A and C. The group B presented smaller contraction amplitudes than did groups A and C, in electrically stimulated contractions. Conclusions: That oxidative stress is implicated in the damage to the detrusor musculature following a period of chronic intravesical obstruction. We show, for the first time, that administration of an antioxidant prior to and following the start of chronic obstruction makes it possible to avoid the cellular lesions that cause detrusor dysfunction.

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“…[2][3][4][5][6] In published studies similar to PBOO, in situ models of ischemia/reperfusion (I/R) also induced the generation of free radicals, which resulted in significant damage to the cellular and subcellular membranes, especially those of the nerves and mitochondria. [7][8][9] The authors of previous studies have demonstrated that natural products with strong antioxidant properties, such as Kohki tea, 10,11 grapes 12,13 and pygeum africanum 14,15 have protective effects against the damage mediated by both PBOO and in vivo and models of I/R. In a recent study, we evaluated the efficacy of the administration of two popular antioxidant agents, coenzyme Q10 and alpha lipoic acid, as a treatment for obstructive bladder dysfunction.…”

Section: Introductionmentioning

confidence: 99%

“…The authors of previous studies have demonstrated that natural products with strong antioxidant properties, such as Kohki tea, 10,11 grapes 12,13 and pygeum africanum 14,15 have protective effects against the damage mediated by both PBOO and in vivo and models of I/R. In a recent study, we evaluated the efficacy of the administration of two popular antioxidant agents, coenzyme Q10 and alpha lipoic acid, as a treatment for obstructive bladder dysfunction 16 .…”

Section: Introductionmentioning

confidence: 99%

Treatment of Obstructive Bladder Dysfunction in Rabbits with Coenzyme Q10 and Alpha Lipoic Acid

Hydery

Schuler

Leggett

et al. 2009

LUTS

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Objectives: Partial bladder outlet obstruction (PBOO) is one of the major pathological effects of benign prostatic hyperplasia in men. It is well demonstrated that ischemia followed by reperfusion and the resulting generation of free radicals and oxidative damage to proteins occur in our rabbit model of PBOO and are involved in the observed progressive obstructive bladder dysfunction. The aim of the current project was to determine if treatment of obstructed rabbits with alpha lipoic acid and coenzyme Q10 can reverse the urodynamic, biochemical, and physiological responses to PBOO. Methods: Twenty adult NZW male rabbits were divided into four groups (n = 5 per group): control (sham surgical) animals with no treatment (group 1); control animals treated with coenzyme Q10 + alpha lipoic acid for 3 weeks beginning 4 weeks after surgery (group 2); 7‐week obstructed rabbits (group 3); and 7‐week obstructed rabbits treated for the last 3 weeks with the combination of the two drugs (group 4). Results: Bladder weight, compliance, volume at micturition and contractile response to all forms of stimulation were reduced following obstruction, but significantly improved after treatment. In addition, the level of reactive oxygen species and reactive nitrogen species damage was significantly reduced by treatment. Conclusion: Our results provide additional support for the beneficial effects of treatment of PBOO with strong antioxidants.

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Oral Kohki Tea and its protective effect against in vitro ischemic damage to the bladder

Abstract: These data are consistent with the concept that Kohki Tea acts by protecting the bladder from cellular damage caused by hypoxia and the generation of free radicals.

Cited by 12 publications

References 34 publications

Urethral monopolar cauterization: alternative infravesical obstruction model in male rats

Urethral monopolar cauterization: alternative infravesical obstruction model in male rats

Influence of oxidative stress on inducing micturition dysfunction following chronic infravesical obstruction and the protective role of an antioxidant diet - association of in vivo and in vitro studies in rats

Treatment of Obstructive Bladder Dysfunction in Rabbits with Coenzyme Q10 and Alpha Lipoic Acid

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