Oral mucosa stem cells alleviates spinal cord injury-induced neurogenic bladder symptoms in rats

Abstract

BackgroundSpinal cord injury (SCI) deteriorates various physical functions, in particular, bladder problems occur as a result of damage to the spinal cord. Stem cell therapy for SCI has been focused as the new strategy to treat the injuries and to restore the lost functions. The oral mucosa cells are considered as the stem cells-like progenitor cells. In the present study, we investigated the effects of oral mucosa stem cells on the SCI-induced neurogenic bladder in relation with apoptotic neuronal cell death and cell proliferation.ResultsThe contraction pressure and the contraction time in the urinary bladder were increased after induction of SCI, in contrast, transplantation of the oral mucosa stem cells decreased the contraction pressure and the contraction time in the SCI-induced rats. Induction of SCI initiated apoptosis in the spinal cord tissues, whereas treatment with the oral mucosa stem cells suppressed the SCI-induced apoptosis. Disrupted spinal cord by SCI was improved by transplantation of the oral mucosa stem cells, and new tissues were increased around the damaged tissues. In addition, transplantation of the oral mucosa stem cells suppressed SCI-induced neuronal activation in the voiding centers.ConclusionsTransplantation of oral mucosa stem cells ameliorates the SCI-induced neurogenic bladder symptoms by inhibiting apoptosis and by enhancing cell proliferation. As the results, SCI-induced neuronal activation in the neuronal voiding centers was suppressed, showing the normalization of voiding function.

Blocking of BDNF-TrkB signaling inhibits the promotion effect of neurological function recovery after treadmill training in rats with spinal cord injury

“…At the end of the experiment, four rats in each group were anesthetized by the inhalation of isoflurane and perfused intracardially with 200 mL normal saline, followed by 250 mL of 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Then, the lumbar segments of the spinal cord (L2-L5) [19] were fixed with 4% paraformaldehyde for 12 h. Subsequently, the spinal cords were paraffin-embedded and sliced transversely into 5 μm sections [26].…”

Human‐ and mouse‐derived neurons can be simultaneously obtained by co‐cultures of human oral mucosal stem cells and mouse neural stem cells

“…Dental pulp was considered as a convenient source of SC, yet in order to harvest SC from the pulp, tooth vitality is irreparably damaged or the tooth needs to be extracted. The regenerative ability of nasal mucosa stem cells has been recently well proven: in a parkinsonian rat model (Müller et al., ), in an hemi‐parkinsonian rat model (Ganz, Arie, Buch, et al.s, ) and in a spinal cord injury model (Cho et al., ). Moreover, hOMSC in an experimental rat model of sciatic nerve injury revealed a neuroprotective effect, improving the motor function following transplantation (Ganz, Arie, Ben‐Zur, et al., ).…”

“…Moreover, the excellent potential of hOMSC has been shown by the fact that they can differentiate towards ectodermal, mesodermal and endodermal cell lineages (Marynka‐Kalmani et al., ). hOMSC transplanted into various neural damage mouse models resulted in clinical improvement and possible regeneration of the neural tissue (Cho et al., ; Ganz, Arie, Buch, et al.s, ). As hOMSC are very promising candidates for transplantation in the mouse model of brain ischaemia, in which regenerative process includes reactivation of neurogenesis, the main aim of this study was to perform the analyses by observing interactions between hOMSC and mNSC cell populations in vitro.…”

Afferent Pathway-Mediated Effect of α1 Adrenergic Antagonist, Tamsulosin, on the Neurogenic Bladder After Spianl Cord Injury

“…The SCI model was induced as previously described [ 19 ]. The rats were anesthetized by inhalation of isoflurane (2% isoflurane in 30% O 2 and 70% N 2 , JW pharmaceutical, Seoul, Korea) during surgery.…”

“…The animals were sacrificed immediately after determining their voiding function by cystometry as previously described [ 19 , 20 ]. For the histological examination, the rats were transcardially perfused with 50mM phosphate-buffered saline (PBS), followed by 4% paraformaldehyde in 100mM sodium phosphate buffer at pH 7.4.…”