Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing

Jerônimo, Márcio Sousa; Barros, Adria do Prado; MoritaI, Vinícius Elord Zen; Alves, Erika Oliveira; Souza, Nathalia Lobão Barroso de; Almeida, Rosane Mansan de; Nóbrega, Yanna Karla de Medeiros; Neto, Florêncio Figueiredo Cavalcanti; Amorin, Rivadávio Fernandes Batista de; Borin, Maria de Fátima; Bocca, Anamélia Lorenzetti

doi:10.1590/s0102-865020160090000003

Cited by 13 publications

(5 citation statements)

References 27 publications

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“…Arginine with a dose of 8 mM effectively decreased IL-8 in the IPEC-J2 cells induced by LPS ( Lan et al , 2020 ). Another study also found that the administration of arginine topical showed low levels of IL-8 in laparotomy mice ( Jeronimo et al , 2016 ).…”

Section: Discussionmentioning

confidence: 92%

“…This result is in line with a study by Zhou et al (2012) in endotoxemic rats, which showed a decrease in IL-10. Arginine supplementation only showed that topical administration did not affect IL-10 production in mice with laparotomy ( Jeronimo et al , 2016 ). In Contrary, several studies showed that glutamine only affects the high production of IL-10 in lobectomy patients ( Wang et al , 2018 ) and in bone marrow-derived macrophages induced by LPS ( de Oliveira et al , 2018 ).…”

Section: Discussionmentioning

confidence: 99%

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Effects of glutamine and arginine combination on pro- and anti-inflammatory cytokines

Maulydia¹,

Rehatta²,

Sudarmo³

2023

Open Vet J

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Background: Cytokines were beneficial for diagnosis and treatment, which in clinical situations introduced from the perspective of pro and anti-inflammatory effects. An inflammatory response is commonly associated with various severe traumatic insults that consequently recruit the immune cells into the target organs and causing systemic inflammatory response that can lead to sepsis. Immune-modulating nutrients, such as glutamine and arginine, are known as pathophysiological modulate in inflammation. Aim: The aim of this study was to evaluate the effect of oral gavage supplementation with a combination of glutamine and arginine on inflammatory cytokines in intestinal mucosa, specifically jejunum. Methods: Sixteen Rattus norvegicus rats (average weight 150–200 g) were randomly divided into two groups: groups A and B, both intraperitoneal injected by 2 ml NaCl 0.9%. Group A orally supplemented with 1 ml dextrose 5% daily, meanwhile, group B orally supplemented with 1 ml combination of glutamine and arginine (contains 250 mg/kg glutamine and 250 mg/kg arginine) daily. The experiment lasted for 3 days. We compared the pro and anti-inflammatory cytokines (IL-10, NF-κB, TNF-α, IL-8, and MMP-8) between the two groups by the Mann–Whitney test. Results: More IL-10, TNF-α, and IL-8 cytokine-produced cells found in group A. Group B produced significantly lower TNF-α ( p = 0.009) and IL-8 ( p = 0.003). The number of NF-κB and MMP-8 were slightly higher in group B. Conclusion: Giving a combination of glutamine and arginine as nutrition supplementation has beneficial effects in decreasing almost half of the cells that produce TNF-α and IL-8. Further studies must be carried out to support a standard guideline for this recommendation.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Effects of glutamine and arginine combination on pro- and anti-inflammatory cytokines

Maulydia¹,

Rehatta²,

Sudarmo³

2023

Open Vet J

View full text Add to dashboard Cite

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“…l -arginine orally or topically improved wound repair; however, the oral administration showed a higher NOS and TGF-β expression, and a significant decrease in inflammatory response. In addition, some studies showed that systemic l -arginine was more efficient than topical l -arginine in wound healing (Zandifar et al 2015 ; Jerônimo et al 2016 ; Witte et al 2002 ; Heffernan et al 2006 ). For this reason, the oral supplement was our option to treat the patients.…”

Section: Discussionmentioning

confidence: 99%

l-Arginine is a feasible supplement to heal chronic anal fissure via reducing internal anal sphincter pressure: a randomized clinical trial study

et al. 2022

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The hypertonicity of internal anal sphincter resting pressure is one of the main causes of chronic anal fissure. Therefore, the aim of this study was to assess the effect of oral administration of l -arginine on the improvement of the anal fissures by relaxing the internal anal sphincter. Seventy-six chronic anal fissure patients (aged 18–65 years) who were referred to Rasoul-e-Akram Hospital, Tehran, Iran from February 2019 to October 2020 participated in this randomized, double-blind, placebo-controlled trial. Participants were allocated into treatment ( l -arginine) and placebo groups. They took a 1000 mg capsule three times a day for 1 month, and then we followed them at the end of the first and third months after the intervention. Clinical symptoms, anal sphincter resting pressure, and quality of life (QoL) were completed at baseline and the end of the study. The analysis of data showed a significant decrease in bleeding, fissure size, and pain for each group; however, in the L-arginine group was more than the control group at the end of the study ( P values < 0.001). Following that, a significant increase in QoL was seen just in patients treated with l -arginine ( P value = 0.006). In addition, the comparison of anal pressures at baseline and, between groups at the end of the study showed a significant reduction in sphincter pressure in patients treated with l -arginine ( P value < 0.001, = 0.049; respectively). The oral administration of 3000 mg l -arginine can heal chronic anal fissures by reducing internal anal sphincter pressure with more negligible side effects. However, we recommend long-term study with more extended follow-up. Clinical trial registry: IRCT20190712044182N1 at Iranian clinical trials, date: 2019-08-27.

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“…Admittedly, oral Haruan supplement has a higher amount of concentrate which is believed to yield more merits compared to eating the flesh itself. Besides, for surgical or major traumatic wounds that involve multiple tissue layers, oral administration of Haruan extract is deemed superior to topical application [65,66]. The detailed steps in the preparation of Haruan capsules are described in Figure 4.…”

Section: Haruan Capsulesmentioning

confidence: 99%

HaruanExtract (Channa striatus) as an Effective Mediator in Promoting Wound Healing

Musa¹,

Min²

2022

Skin Grafts for Successful Wound Closure

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Wound healing remains a major issue in surgery. None of the existing treatment modalities in caring for wounds can yet claim to be the holy grail of wound management. Channa striatus, locally known in Malaysia as Haruan, is a freshwater air-breathing carnivorous fish that is proven to influence the different phases of wound healing. As a medicinal fish, not only does Haruan have a high content of amino and fatty acids, which are essential in collagen fibre synthesis during wound recovery, it also abounds in arachidonic acid and polyunsaturated fatty acids that promote prostaglandin synthesis, a vital component of the healing process. Moreover, its antinociceptive effects could potentially reduce wound pain, an important factor in wound healing. Proteomic studies show that a quarter of the total protein detected in freeze- and spray-dried C. striatus extract are actin, myosin and tropomyosin – all molecules that play a role in the wound healing process. Proteomic profiling also reveals that Haruan possesses two types of collagen namely collagen type-I and type-II that confer tensile strength during the healing process. It is proven that collagen along with other components of the extracellular matrix form the granulation tissue which, when contracted, closes the wound and concomitantly aligns the collagen fibres in the extracellular matrix. Hence, it is inferred that Haruan promotes the maturation of granulation tissue, thereby expediting the wound healing process itself. Consequently, it could mediate a faster recovery from surgical wound coupled with a lower incidence of wound infection due to an improved and accelerated wound healing process. Additionally, Haruan has demonstrated its ability in promoting angiogenesis and cell proliferation in wound bed preparation for skin grafting. Furthermore, a Haruan aerosol concentrate can act as a wound dressing at the donor site thereby enhancing the healing process while simultaneously exhibiting some antinociceptive properties. Haruan’s exceptional ability in promoting wound healing together with its potential use in skin grafting would be instrumental in the field of surgery. In essence, the cumulated benefits from all the processes involved would translate into a significant reduction of hospitalisation cost; that would immensely benefit not only the patient, but also the government.

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Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing

Cited by 13 publications

References 27 publications

Effects of glutamine and arginine combination on pro- and anti-inflammatory cytokines

Effects of glutamine and arginine combination on pro- and anti-inflammatory cytokines

l-Arginine is a feasible supplement to heal chronic anal fissure via reducing internal anal sphincter pressure: a randomized clinical trial study

HaruanExtract (Channa striatus) as an Effective Mediator in Promoting Wound Healing

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