Oral plasma proteins attenuate gut inflammatory effects induced by S. aureus enterotoxin B challenge in rats

Pérez-Bosque, Anna; Miró, Lluïsa; Polo, Javier; Russell, Louis; Campbell, Joy; Weaver, Eric; Crenshaw, J. D.; Moretó, Miquel

doi:10.1016/j.livsci.2010.06.076

Cited by 10 publications

(10 citation statements)

References 13 publications

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“…SDP stimulates Tlr2 expression and expression of both Il-10 and Tgf-β, consistent with previous observations in healthy unchallenged rodents 53 , and these two variables may be correlated. The capacity of Tlr2 to induce proor anti-inflammatory responses depends on whether it dimerizes with other receptors.…”

Section: Discussionsupporting

confidence: 91%

“…Blautia has also been shown to regulate the expression of tight-junction ZO-1 and maintenance of mucosal permeability 52 and L. frumenti is a commensal species that, when given by oral gavage to piglets prior to weaning, decreases the relative abundance of opportunistic pathogens and improves intestinal mucosal integrity via a mechanism involving up-regulation of ZO-1, occludin, and claudin-1. We have previously shown that inflammation reduces the expression of the tight junction protein ZO-1 and adherent junction protein β-catenin in the small intestine and that SDP can prevent this decrease 50,53 . Because Blautia was increased 1.4-fold by SDP, we hypothesized that the effects of SDP on mucosal integrity might be mediated by this species.…”

Section: Discussionmentioning

confidence: 98%

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Dietary supplementation with spray-dried porcine plasma has prebiotic effects on gut microbiota in mice

Moretó

Miró

Amat

et al. 2020

Sci Rep

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In animal models of inflammation and in farm animals, dietary inclusion of spray-dried porcine plasma (SDP) reduces mucosal inflammation. Here, we study whether these effects could be mediated by changes in the intestinal microbiota and if these changes are similar to those induced by oral antibiotics. Weaned 21-day-old C57BL/6 mice were divided into 3 groups: the CTL group, fed the control diet; the COL group, administered low doses of neomycin and colistin; and the SDP group, supplemented with 8% SDP. After 14 days, analysis of the fecal microbiome showed that the microbiota profiles induced by SDP and the antibiotics were very different, thus, SDP has prebiotic rather than antibiotic effects. At the phylum level, SDP stimulated the presence of Firmicutes, considerably increasing the lactobacilli population. It also enhanced the growth of species involved in regulatory T-lymphocyte homeostasis and restoration of the mucosal barrier, as well as species negatively correlated with expression of proinflammatory cytokines. At the mucosal level, expression of toll-like receptors Tlr2, Tlr4 and Tlr9, and mucous-related genes Muc2 and Tff3 with regulatory and barrier stability functions, were increased. SDP also increased expression of Il-10 and Tgf-β, as well as markers of macrophages and dendritic cells eventually promoting an immune-tolerant environment. open Scientific RepoRtS | (2020) 10:2926 | https://doi.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Dietary supplementation with spray-dried porcine plasma has prebiotic effects on gut microbiota in mice

Moretó

Miró

Amat

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The KO mice showed a notable increase in both MUC1 and MUC4 RNA expression, consistent with results obtained in humans (12), whereas the dietary SBI attenuated these effects. There is evidence that the effects of SBI on mucus and mucin secretion involve interactions of dietary Ig with the microbiome; for example, plasma supplements can restore the secretion of antimicrobial peptides in inflamed animals (30), they can bind and neutralize endotoxin and other microbial components (33), and they can also affect the microbiota profile in both humans (4) and rats (19). These changes can modify the cross talk between the host and the microbiota and lead to an improvement in permeability, inflammation, and the function of the mucus layer.…”

Section: Fitc-dextranmentioning

confidence: 99%

Dietary intervention with serum-derived bovine immunoglobulins protects barrier function in a mouse model of colitis

Pérez-Bosque

Miró

Maijó

et al. 2015

American Journal of Physiology-Gastrointestinal and Liver Physi

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Dietary supplementation with immunoglobulins from animal plasma has anti-inflammatory effects on intestinal and lung models of acute inflammation. Here, we aimed to establish whether dietary intervention with serum-derived bovine immunoglobulin (SBI) can prevent alterations in intestinal barrier function in a mouse model with a genetic predisposition to inflammatory bowel disease (IBD). Wild-type (WT) mice and mice lacking the mdr1a gene (KO) were fed diets supplemented with either SBI (2% wt/wt) or milk proteins (control diet), from day 21 (weaning) until day 56. The epithelial permeability of distal colon crypts was measured by confocal microscopy using a fluorescent marker. The expression of junctional epithelial E-cadherin and β-catenin proteins were determined by Western blot and zonula occludens-1 (ZO-1) by immunofluorescence. Mucins (MUC1, MUC2, MUC4), TFF3, cytokines (TNF-α, IFN-γ), and inducible nitric oxide synthase RNA expression were quantified by real-time PCR. SBI blocked the increase in colon crypt permeability and partially prevented the reduction in E-cadherin and ZO-1 expression that characterize the KO mouse model (both P < 0.05). SBI inclusion also reduced the mucosal expression of the inflammatory markers TNF-α, IFN-γ, and inducible nitric oxide synthase (all P < 0.005). The number of goblet cells in the colon of KO mice was low and correlated well with MUC2 and TFF3 expression (P < 0.001), whereas dietary supplementation with SBI attenuated these effects (all P < 0.05). In short, dietary SBI ameliorated colonic barrier alterations and reduced the expression of mucosal inflammatory markers in a genetic model of IBD.

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“…Since cryptdin 4 can block IL-1b release from lipopolysaccharide-activated monocytes (42) , a decrease in its expression may increase intestinal IL-1b production. This would make the intestine more susceptible to SEB-induced damage and contribute to inflammatory bowel diseases (41) . The mucosa of the jejunum was mounted in using chambers and incubated with horseradish peroxidase added to the mucosal side (35) .…”

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 99%

A rat model of mild intestinal inflammation induced byStaphylococcus aureusenterotoxin B

Pérez-Bosque

Moretó

2010

Proc. Nutr. Soc.

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The epithelial barrier of the intestine and the gut-associated lymphoid tissue (GALT) protects the host against luminal pathogenic micro-organisms. This is important at weaning, when animals are exposed to infectious agents and stresses. We have developed a rat model of intestinal inflammation post weaning, based on the systemic administration ofStaphylococcus aureusenterotoxin B (SEB). Since the inflammatory response obtained is mild, the food intake pattern is not affected, which makes this model useful for studies of nutritional therapies for intestinal inflammatory disease. SEB increased T-lymphocytes in Peyer's patches and the number of activated T-lymphocytes in mesenteric lymph nodes (organized GALT). In the lamina propria, SEB increased activated T-lymphocytes as well as cytotoxic and natural killer-cell populations of the diffuse GALT. It also increased pro-inflammatory cytokines and inflammatory mediators in both Peyer's patches and mucosa. Rats given SEB had higher paracellular permeability to macromolecules, which was associated with a reduction in epithelial tightness. This model was used to examine whether dietary supplementation with spray-dried animal plasma proteins affects intestinal inflammation. Results showed that dietary plasma proteins can attenuate the mucosal immune response in both organized and diffuse GALT and that these effects are mediated by a reduction in the production of pro-inflammatory cytokines.

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Oral plasma proteins attenuate gut inflammatory effects induced by S. aureus enterotoxin B challenge in rats

Cited by 10 publications

References 13 publications

Dietary supplementation with spray-dried porcine plasma has prebiotic effects on gut microbiota in mice

Dietary supplementation with spray-dried porcine plasma has prebiotic effects on gut microbiota in mice

Dietary intervention with serum-derived bovine immunoglobulins protects barrier function in a mouse model of colitis

A rat model of mild intestinal inflammation induced byStaphylococcus aureusenterotoxin B

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