Oral status of a noble European couple from the 16th century: A morphologic analysis of the teeth of Alessandro Farnese and his wife Maria D’Aviz

Peracchia, Marianna; Meleti, Marco; Armocida, Emanuele; Toffoli, Andrea; Cucurachi, N.; Bussolati, Ovidio; Cecchi, Rossana

doi:10.1127/anthranz/2021/1423

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“…An interesting result is related to the presence of the TMC-34 metabolite deriving from Actinomyces, which are involved in the development of periodontitis 38 – 40 . This metabolite was mainly present in the dental calculus of Maria D’Aviz, and it is known that the noble woman suffered from caries and periodontal diseases 24 .…”

Section: Resultsmentioning

confidence: 99%

“…After opening the lead case containing the remains, the teeth were submitted to morphological analysis. The main feature in the dentition of Alessandro Farnese was severe wear, whereas the teeth of Maria D’Aviz were characterized by the presence of caries and periodontal diseases 24 . These findings could be associated with the different lifestyle of the noble couple, Duke Farnese spending most of his adult lifetime on battlefields all over Europe, and his wife maintaining the habits of the Portuguese court in the city of Parma.…”

Section: Introductionmentioning

confidence: 99%

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Ultra-high performance liquid chromatography high-resolution mass spectrometry for metabolomic analysis of dental calculus from Duke Alessandro Farnese and Maria D’Aviz

Riboni

Bianchi

Mattarozzi

et al. 2023

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Dental calculus is a valuable resource for the reconstruction of dietary habits and oral microbiome of past populations. In 2020 the remains of Duke Alessandro Farnese and his wife Maria D’Aviz were exhumed to get novel insights into the causes of death. This study aimed to investigate the dental calculus metabolome of the noble couple by untargeted metabolomics. The pulverized samples were decalcified in a water-formic acid mixture, extracted using methanol/acetonitrile and analyzed by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) using a reversed-phase separation followed by electrospray ionization and full scan in positive and negative ion mode. Waters Synapt-G2-Si High-Definition hybrid quadrupole time-of-flight mass spectrometer was used. Significant features were then identified using MSE acquisition mode, recording information on exact mass precursor and fragment ions within the same run. This approach, together with data pre-treatment and multivariate statistical analysis allowed for the identification of compounds able to differentiate between the investigated samples. More than 200 metabolites were identified, being fatty acids, alcohols, aldehydes, phosphatidylcholines, phosphatidylglycerols, ceramides and phosphatidylserines the most abundant classes. Metabolites deriving from food, bacteria and fungi were also determined, providing information on the habits and oral health status of the couple.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%