Oral steroids enhance epithelial repair in nasal polyposis via upregulation of the AP-1 gene network

Li, Chun Wei; Cheung, Wai Yiu; Lin, Zhi; Li, Tian Ying; Lim, Joe Thuan; Wang, De Yun

doi:10.1136/thx.2008.106096

Cited by 26 publications

(36 citation statements)

References 34 publications

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“…2 The extent of immunoreactivity of p63 and TAp73 antibodies within the epithelial region was graded as follows: score 0, negative staining; score 1, 1 to 2 layers of positive cells; and score 2, more than 2 layers of positive cells. Overexpression of p63 and TAp73 is defined when the score is 2.…”

Section: Evaluation Of Immunohistochemical Patternsmentioning

confidence: 99%

“…2 The p63-related biological functions and diseases and the p63 upstream and downstream genes were explored by using ingenuity pathways analysis (IPA; version 8.7, Ingenuity Systems, www.ingenuity.com). IPA is a Web-delivered application that enables the easy search and summarization of the scientific literature for a single gene or a group of gene molecules (eg, microarray data).…”

Section: Microarray and Web-based Bioinformatics Analysismentioning

confidence: 99%

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Role of p63/p73 in epithelial remodeling and their response to steroid treatment in nasal polyposis

Zhang

et al. 2011

Journal of Allergy and Clinical Immunology

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Section: Evaluation Of Immunohistochemical Patternsmentioning

confidence: 99%

Section: Microarray and Web-based Bioinformatics Analysismentioning

confidence: 99%

Role of p63/p73 in epithelial remodeling and their response to steroid treatment in nasal polyposis

Zhang

et al. 2011

Journal of Allergy and Clinical Immunology

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“…The management of nasal polyposis has been the subject of frequent controversial debates for many decades [20]. Surgical or medical treatment or both have been recommended as the treatment of choice.…”

Section: Discussionmentioning

confidence: 99%

“…The infiltration of various inflammatory cells, epithelial damage and expression of the cytokines are similar to the pathological profile of asthma. From this perspective, NP could therefore be regarded as a paradigm of chronic airway inflammation [20]. Nasal polyposis is a common nasal disease with a high rate of recurrence [21].…”

Section: Discussionmentioning

confidence: 99%

Does vitamin D have protective effect on human nasal polyposis: histological and immunohistochemical study

Faruk¹,

Yousef²,

Mohamed³

2014

J Histol Histopathol

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Background: Nasal polyps (NPs) are benign pedicled mucosal protrusions into the nasal cavity of multifactorial origin. Vitamin D has been demonstrated as having potential immunomodulatory activity and act as an antiproliferative agents. Aim of the work: To determine the possible antiproliferative effect and immunomodulatory activity of VD on human nasal polyposis. Material and methods: Based on thirty patients and divided equally into 3 groups. Group Ι (healthy subjects). Group ΙΙ were received low daily oral dose of VD(1000 IU) for 4 weeks. Group ΙΙΙ were received high daily oral dose of VD(4000 IU) for 4 weeks.Each group ΙΙ and ΙΙΙ divided into 2 subgroups; group b: patients with NP before taking VD and group a: patients with NP after taking. Nasal biopsies were obtained of all groups for histological examination and immunohistochemical detection of Toll-like receptors 9 expression. Results: patients of nasal polyps before VD taking (groups IIb and IIIb) presented with symptoms of Visual Analogue Scale, VAS score (facial pain, headache, nasal blockage, nasal discharge, post-nasal drip and olfactory disturbance) and endoscopic appearance of Lund and Mackey score (polypi, edema and discharge), damage of respiratory epithelium, extensive accumulation of collagen fibers in lamina propria and highly expressed TLR-9. The high dose VD group (IIIa) showed near normal respiratory epithelium, significant decrease (P<0.05) in all symptoms of VAS score, endoscopic appearance of Lund and Mackey score. The mean area % of submucosal accumulation of collagen fibers and TLR-9 expression were also significant decreased but decrease was insignificant (P<0.05) in the low dose VD group (IIa). Conclusion: VD participate significantly in protection against human nasal polyposis when used by high therapeutic dose, by reducing the size of nasal polyps, relieving the symptoms and signs of nasal polyposis.

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“…It has been proposed that the ability of corticosteroid to elicit a robust anti-inflammatory effect is mainly mediated by suppressing the multiple inflammatory genes that are activated in polyp tissues. Recently, several studies have shown that corticosteroid is able to promote tissue repair and remodeling in nasal polyps and asthma through transforming growth factor-β and epidermal growth factor/epidermal growth factor receptor signaling pathways [11]. However, whether and how corticosteroid affects the epithelial barrier of nasal polyps remains elusive.…”

Section: Discussionmentioning

confidence: 99%

Corticosteroid Regulates Epithelial Occludin Expression in Nasal Polyps through a MKP-1-Dependent Pathway

Chen

Luo

Han

et al. 2014

ORL

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Objective: To evaluate the regulatory effect of corticosteroid on occludin expression in polyp tissues of chronic rhinosinusitis with nasal polyps (CRSwNP) patients and in vitro. Methods: Twenty CRSwNP patients were enrolled and subjected to prednisone (30 mg/day for 14 days). The expression of occludin in polyp tissues was examined before and after treatment using immunohistochemical staining and quantitative reverse transcription polymerase chain reaction. Moreover, the expression of occludin in polyp-derived epithelial cells (PECs) and human bronchial epithelial cells (BECs) was examined using Western blot analysis in the presence of corticosteroid and/or MKP-1 siRNA, respectively. Results: mRNA and protein expression of occludin in polyp tissues was significantly upregulated after prednisone administration (p < 0.05). Corticosteroids significantly increased MKP-1 and occludin expression in cultured PECs (p < 0.05), and MKP-1 siRNA significantly decreased occludin expression in cultured BECs (p < 0.05). Conclusion: Our findings suggest that corticosteroid can promote epithelial occludin expression in nasal polyps through a MKP-1-dependent pathway. i 2014 S. Karger AG, Basel

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Oral steroids enhance epithelial repair in nasal polyposis via upregulation of the AP-1 gene network

Cited by 26 publications

References 34 publications

Role of p63/p73 in epithelial remodeling and their response to steroid treatment in nasal polyposis

Role of p63/p73 in epithelial remodeling and their response to steroid treatment in nasal polyposis

Does vitamin D have protective effect on human nasal polyposis: histological and immunohistochemical study

Corticosteroid Regulates Epithelial Occludin Expression in Nasal Polyps through a MKP-1-Dependent Pathway

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