Oral subchronic exposure to silver nanoparticles in rats

García, Tánia; Lafuente, Daisy; Blanco, Jordi; Sánchez, Domènec J.; Sirvent, Juan J.; Domingo, José L.; Gómez, Mercedes

doi:10.1016/j.fct.2016.04.010

Cited by 57 publications

(50 citation statements)

References 42 publications

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“…Bone marrow, the organ in which micronucleus formation occurs, accumulates high levels of AgNPs that is consistent with the preferential accumulation of AgNPs in reticuloendothelial tissues (Li et al 2014). Likewise, high levels of AgNPs accumulate in the liver (Bergin et al 2016; Boudreau et al 2016; Garcia et al 2016; Loeschner et al 2011; van der Zande et al 2012). Also, AgNPs were detected in developing embryos after exposure of pregnant dams and thus can potentially induce damage to embryos (Austin et al 2012).…”

Section: Methodsmentioning

confidence: 99%

“…Studies in laboratory animals showed that AgNPs are absorbed in the gastrointestinal tract, distribute to different organs and tissues and accumulate in various organs, particularly, in reticuloendothelial organs (liver, spleen, kidney, lung, and other organs) (Loeschner et al 2011; van der Zande et al 2012; Bergin et al 2016; Boudreau et al 2016; Garcia et al 2016). AgNP biodistribution analyzes in pregnant mice and developing embryos showed that AgNPs distribute to most maternal organs, extraembryonic tissues, and embryos (Austin et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, AgNPs can potentially cause damage to multiple tissues. Most studies failed to observe changes in body and/or organ weights, histology, hematology, and blood chemistry after oral exposure to AgNPs of up to 1000 mg/kg daily doses, although increase in liver enzymes, oxidative stress markers, and inflammatory cytokines were noted in some studies (Bergin et al 2016; Boudreau et al 2016; Garcia et al 2016; Kim et al 2008; Park et al 2010; Patlolla, Hackett, and Tchounwou 2015b). Although these data suggest that ingestion of AgNPs results in relatively low general toxicity, they do not specifically address cancer risk.…”

Section: Introductionmentioning

confidence: 99%

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Differential effects of silver nanoparticles on DNA damage and DNA repair gene expression in Ogg1-deficient and wild type mice

et al. 2017

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Due to extensive use in consumer goods, it is important to understand the genotoxicity of silver nanoparticles (AgNPs) and identify susceptible populations. 8-Oxoguanine DNA glycosylase 1 (OGG1) excises 8-oxo-7,8-dihydro-2-deoxyguanine (8-oxoG), a pro-mutagenic lesion induced by oxidative stress. To understand whether defects in OGG1 is a possible genetic factor increasing an individual’s susceptibly to AgNPs, we determined DNA damage, genome rearrangements, and expression of DNA repair genes in Ogg1-deficient and wild type mice exposed orally to 4 mg/kg of citrate-coated AgNPs over a period of 7 d. DNA damage was examined at 3 and 7 d of exposure and 7 and 14 d post-exposure. AgNPs induced 8-oxoG, double strand breaks (DSBs), chromosomal damage, and DNA deletions in both genotypes. However, 8-oxoG was induced earlier in Ogg1-deficient mice and 8-oxoG levels were higher after 7-d treatment and persisted longer after exposure termination. AgNPs downregulated DNA glycosylases Ogg1, Neil1, and Neil2 in wild type mice, but upregulated Myh, Neil1, and Neil2 glycosylases in Ogg1-deficient mice. Neil1 and Neil2 can repair 8-oxoG. Thus, AgNP-mediated downregulation of DNA glycosylases in wild type mice may contribute to genotoxicity, while upregulation thereof in Ogg1-deficient mice could serve as an adaptive response to AgNP-induced DNA damage. However, our data show that Ogg1 is indispensable for the efficient repair of AgNP-induced damage. In summary, citrate-coated AgNPs are genotoxic in both genotypes and Ogg1 deficiency exacerbates the effect. These data suggest that humans with genetic polymorphisms and mutations in OGG1 may have increased susceptibility to AgNP-mediated DNA damage.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential effects of silver nanoparticles on DNA damage and DNA repair gene expression in Ogg1-deficient and wild type mice

et al. 2017

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“…26 Other studies have also reported some adverse impacts on liver and kidney function of mice upon repeated ingestion of silver nanoparticles. 27,28 It was suggested that 125 mg/kg of silver nanoparticles may be the limit above which adverse effects on liver may be observed. 28 In summary, animal studies have shown that silver nanoparticles may accumulate in the body and have toxic effects when ingested at sufficiently high levels, but it is not clear whether these levels are close to those actually achievable through food consumption.…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

“…27,28 It was suggested that 125 mg/kg of silver nanoparticles may be the limit above which adverse effects on liver may be observed. 28 In summary, animal studies have shown that silver nanoparticles may accumulate in the body and have toxic effects when ingested at sufficiently high levels, but it is not clear whether these levels are close to those actually achievable through food consumption. In future studies, it will therefore be important to carry out long-term chronic toxicity studies using nanoparticle levels that are more similar to those actually consumed in the human diet.…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

2017

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Nanotechnology offers the food industry a number of new approaches for improving the quality, shelf life, safety, and healthiness of foods. Nevertheless, there is concern from consumers, regulatory agencies, and the food industry about potential adverse effects (toxicity) associated with the application of nanotechnology in foods. In particular, there is concern about the direct incorporation of engineered nanoparticles into foods, such as those used as delivery systems for colors, flavors, preservatives, nutrients, and nutraceuticals, or those used to modify the optical, rheological, or flow properties of foods or food packaging. This review article summarizes the application of both inorganic (silver, iron oxide, titanium dioxide, silicon dioxide, and zinc oxide) and organic (lipid, protein, and carbohydrate) nanoparticles in foods, highlights the most important nanoparticle characteristics that influence their behavior, discusses the importance of food matrix and gastrointestinal tract effects on nanoparticle properties, emphasizes potential toxicity mechanisms of different food-grade nanoparticles, and stresses important areas where research is still needed. The authors note that nanoparticles are already present in many natural and processed foods, and that new kinds of nanoparticles may be utilized as functional ingredients by the food industry in the future. Many of these nanoparticles are unlikely to have adverse affects on human health, but there is evidence that some of them could have harmful effects and that future studies are required.

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Toxicity of Nanomaterials to the Gastrointestinal Tract

Rice

2022

Toxicology of Nanoparticles and Nanomaterials in Human, Terrestrial and Aquatic Systems

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Oral subchronic exposure to silver nanoparticles in rats

Cited by 57 publications

References 42 publications

Differential effects of silver nanoparticles on DNA damage and DNA repair gene expression in Ogg1-deficient and wild type mice

Differential effects of silver nanoparticles on DNA damage and DNA repair gene expression in Ogg1-deficient and wild type mice

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

Toxicity of Nanomaterials to the Gastrointestinal Tract

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