Oral supplementation of<i>trans</i>-cinnamaldehyde reduces uropathogenic<i>Escherichia coli</i>colonization in a mouse model

Narayanan, Amoolya; Muyyarikkandy, Muhammed Shafeekh; Mooyottu, Shankumar; Venkitanarayanan, Kumar; Amalaradjou, Mary Anne Roshni

doi:10.1111/lam.12713

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…Additionally, no cytotoxic effects on human epithelial cells at the tested concentrations were reported previously with TC and EG ( Amalaradjou et al, 2010 ; Karumathil et al, 2016 ). Moreover our previous studies revealed that in-feed supplementation of TC and EG in broiler chicks ( Kollanoor-Johny et al, 2012 ) and TC in mice ( Narayanan et al, 2017 ) for 10 days did not result in any toxicity.…”

Section: Discussionmentioning

confidence: 93%

Trans-Cinnamaldehyde and Eugenol Increase Acinetobacter baumannii Sensitivity to Beta-Lactam Antibiotics

et al. 2018

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Multi-drug resistant (MDR) Acinetobacter baumannii is a major nosocomial pathogen causing a wide range of clinical conditions with significant mortality rates. A. baumannii strains are equipped with a multitude of antibiotic resistance mechanisms, rendering them resistant to most of the currently available antibiotics. Thus, there is a critical need to explore novel strategies for controlling antibiotic resistance in A. baumannii. This study investigated the efficacy of two food-grade, plant-derived antimicrobials (PDAs), namely trans-cinnamaldehyde (TC) and eugenol (EG) in decreasing A. baumannii’s resistance to seven β-lactam antibiotics, including ampicillin, methicillin, meropenem, penicillin, aztreonam, amoxicillin, and piperacillin. Two MDR A. baumannii isolates (ATCC 17978 and AB 251847) were separately cultured in tryptic soy broth (∼6 log CFU/ml) containing the minimum inhibitory concentration (MIC) of TC or EG with or without the MIC of each antibiotic at 37°C for 18 h. A. baumannii strains not exposed to the PDAs or antibiotics served as controls. Following incubation, A. baumannii counts were determined by broth dilution assay. In addition, the effect of PDAs on the permeability of outer membrane and efflux pumps in A. baumannii was measured. Further, the effect of TC and EG on the expression of A. baumannii genes encoding resistance to β-lactam antibiotics (blaP), efflux pumps (adeABC), and multi-drug resistant protein (mdrp) was studied using real-time quantitative PCR (RT-qPCR). The experiment was replicated three times with duplicate samples of each treatment and control. The results from broth dilution assay indicated that both TC and EG in combination with antibiotics increased the sensitivity of A. baumannii to all the tested antibiotics (P < 0.05). The two PDAs inhibited the function of A. baumannii efflux pump, (AdeABC), but did not increase the permeability of its outer membrane. Moreover, RT-qPCR data revealed that TC and EG down-regulated the expression of majority of the genes associated with β-lactam antibiotic resistance, especially blaP and adeABC (P < 0.05). The results suggest that TC and EG could potentially be used along with β-lactam antibiotics for controlling MDR A. baumannii infections; however, their clinical significance needs to be determined using in vivo studies.

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Section: Discussionmentioning

confidence: 93%

Trans-Cinnamaldehyde and Eugenol Increase Acinetobacter baumannii Sensitivity to Beta-Lactam Antibiotics

et al. 2018

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“…Si des études supplémentaires seront nécessaires pour étudier les mécanismes sous-jacents à cette synergie, une association canneberge-cannelle pourrait être une stratégie prometteuse pour limiter les IU et le recours aux antibiothérapies. Par ailleurs, la cannelle a d'autres propriétés qui pourraient également être intéressantes en cas d'IU comme une action anti-inflammatoire [17,[20][21][22][23]. Les données disponibles dans la littérature sur les effets antimicrobiens de la cannelle concernent principalement le transcinnamaldéhyde présent essentiellement dans l'huile essentielle de cannelle.…”

Section: Discussionunclassified

“…Les données disponibles dans la littérature sur les effets antimicrobiens de la cannelle concernent principalement le transcinnamaldéhyde présent essentiellement dans l'huile essentielle de cannelle. Il a été démontré qu'une supplémentation par voie orale en transcinnamaldéhyde réduisait la colonisation par une souche uropathogène d'Escherichia coli dans un modèle murin [21]. Utilisé comme agent de traitement des surfaces ou dans les solutions de remplissage des cathéters, le transcinnamaldéhyde pourrait limiter les IU chez l'homme contractées en milieu hospitalier [20].…”

Section: Discussionunclassified

Effet synergique d’extraits de cannelle et de canneberge sur l’inhibition de l’adhésion d’Escherichia coli uropathogène aux cellules épithéliales de la vessie

Leblanc¹,

Blondeau²,

Holowacz³

et al. 2019

Phytothérapie

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L’effet inhibiteur des proanthocyanidines (PACs) de type A de la canneberge sur l’adhésion d’Escherichia coli aux cellules uroépithéliales est bien documenté. Cette adhésion étant une des étapes précoces des infections urinaires (IU), la canneberge est utilisée dans la prévention de ces infections. La cannelle étant une autre source alimentaire de PACs de type A, nous avons testé son potentiel antiadhésif dans un modèle in vitro de cellules épithéliales de vessie humaine (lignée cellulaire T24). Dans ce modèle, un extrait de cannelle de Ceylan standardisé à plus de 8 % de PACs de type A2 a inhibé l’adhésion d’Escherichia coli. L’effet observé était dépendant de la dose de PACs. Les tests effectués en association avec un extrait de canneberge ont montré un effet synergique entre les deux extraits associés en certaines proportions. Ces résultats suggèrent que la cannelle pourrait être utile dans la prise en charge des IU.

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“…Prorok et al [5] found that cinnamic acid has a protective effect on the nerve of the Parkinson's disease (PD) mouse induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), which may be achieved by activating peroxisome proliferator-activated receptor-a (PPARa) to protect the loss of dopamine neurons. Narayanan et al [6] confirmed that prophylactic supplementation of transcinnamaldehyde (TC) significantly reduced the uropathogenic Escherichia coli (UPEC) colonization in the urinary tract infections (UTIs).…”

Section: Introductionmentioning

confidence: 99%

The traditional uses, phytochemistry, pharmacology and toxicology of Cinnamomi ramulus: a review

Liu

Zhang

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Cinnamomi ramulus (called Guizhi in Chinese) is a traditional medicine used to treat gastrointestinal dysfunction, cancer, arthritis, osteoporosis, spleen deficiency, Alzheimer's disease and obesity. This review aimed to provide a systematic summary on the geographical distribution, botany, traditional application, phytochemistry, pharmacology, pharmacokinetics, toxicology and other aspects of Cinnamomi ramulus. Key finding So far, more than 121 chemical compounds have been isolated from Cinnamomi ramulus, including volatile oil, organic acids, triterpenoid saponins, coumarins, tannins, flavonoids and flavonoid glycosides, steroids and polysaccharides. This paper reviews the pharmacological effects of Cinnamomi ramulus on antibacterial, anti-inflammatory, antiviral, antitumour, antipyretic and analgesic, antidiabetic and antiplatelet aggregation effects. Furthermore, the present review also indicates that Cinnamomi ramulus has the potential to develop into drugs for treating various diseases with high efficacy and low toxicity. Summary The convictive evidence from modern pharmacology research supports the traditional application of Cinnamomi ramulus. However, further studies on the structure-activity relationship of some of the isolated compounds may improve their biological potency. More toxicological studies will also contribute to the progress of clinical trial studies.

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Oral supplementation oftrans-cinnamaldehyde reduces uropathogenicEscherichia colicolonization in a mouse model

Cited by 14 publications

References 23 publications

Trans-Cinnamaldehyde and Eugenol Increase Acinetobacter baumannii Sensitivity to Beta-Lactam Antibiotics

Trans-Cinnamaldehyde and Eugenol Increase Acinetobacter baumannii Sensitivity to Beta-Lactam Antibiotics

Effet synergique d’extraits de cannelle et de canneberge sur l’inhibition de l’adhésion d’Escherichia coli uropathogène aux cellules épithéliales de la vessie

The traditional uses, phytochemistry, pharmacology and toxicology of Cinnamomi ramulus: a review

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