Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats

Abstract

To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.

Efeitos da arginina na reparação cicatricial da parede abdominal em ratos Wistar.

“…The possible mechanism is related to the dual function of promoting angiogenesis and arteriogenesis, in addition to regulating collagen expression 28 .…”