Oral Systemic Administration of Ankaferd Blood Stopper Has No Short-Term Toxicity in an In Vivo Rabbit Experimental Model

Bilgili, Hasan; Captug, Ozge; Koşar, Ali; Kurt, Mevlüt; Kekilli, Murat; Shorbagi, Ali; Kurt, Ozlem Kar; Özdemir, Oktay; Göker, Hakan; Haznedaroğlu, İbrahim C.

doi:10.1177/1076029609335912

Cited by 46 publications

(55 citation statements)

References 14 publications

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“…The researchers suggested that ABS stimulates the formation of an encapsulated protein network that provides a focal point for erythrocyte aggregation [2,6,25]. The short-term hematological and biochemical safety of oral systemic ABS in rabbits have been reported [13]. Acute mucosal toxicity, hematotoxicity, hepatotoxicity, nephrotoxicity, and biochemical AR AR is a member of the nuclear receptor superfamily, members of which function as ligand-inducible transcription factors that mediate expression of target genes in response to ligands specific to each receptor, including steroids, retinoids, vitamin D, and thyroid hormone [38].…”

Section: Discussionmentioning

confidence: 99%

“…toxicity were not observed during the short-term (7 days) follow-up period [13]. Use of ABS as a hemostatic agent in external hemorrhages and in dental treatment in humans provided the first data showing that ABS was safe and effective in humans [9].…”

Section: Nf-κbmentioning

confidence: 99%

“…The hemostatic effects of ABS have been observed in vitro and in vivo [12][13][14][15][16][17][18]. Use of ABS as a hemostatic agent in external hemorrhages and in dental treatment in humans provided the first data showing that ABS was safe and effective in humans [9].…”

Section: Introductionmentioning

confidence: 99%

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The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Yılmaz¹,

Güleç²,

Torun³

et al. 2011

Turk J Hematol

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Objective: Ankaferd ® Blood Stopper (ABS) is an herbal extract that has historically been used as a hemostatic agent in traditional Turkish medicine. ABS is comprised of a standardized herbal mixture of T. vulgaris, G. glabra, V. vinifera, A. officinarum, and U. dioica. ABS's basic mechanism of action is the formation of an encapsulated protein web, which represents the focal point for vital erythrocyte masses. The hemostatic effects of ABS have been observed in vitro and in vivo. ABS was registered as a hemostatic agent for external hemorrhages and dental bleeding following phase I randomized, double-blind crossover placebo-controlled clinical research, and safety and efficacy reports. In terms of the potential use of ABS, transcription factors may be novel factors that play a role in the hemostatic and other pleiotropic effects of ABS. Materials and Methods: Hence, the present study aimed to investigate the effects of ABS on endothelium, and possible transcription factor changes in HUVEC (human umbilical vein endothelial cells) and the erythrocyte membrane profile. ABS (5 μL and 50 μL) was administered to HUVEC (in 75 cm 2 ; ~75% fullness) for 5 min and 15 min. Results: ABS caused significant increases in the level of activation of the following transcription factors; AP2, AR, CRE/ATF1, CREB, E2F1-5, E2F6, EGR, GATA, HNF-1, ISRE, Myc-Max, NF-1, NFkB, p53, PPAR, SMAD 2/3, SP1, TRE/AP1, and YY1. Following erythrocyte membrane isolation, protein complexes were undissolved, but denatured. The protein complex formed was resistant to heat and detergent. Trypsin and sonication were used in order to break this complex; the complex dissolved and erythrocyte membrane proteins were released in SDS-PAGE. Conclusion: ABS established a very fast and solid protein web, and increased the level of transcription factor activation. Therefore the cellular effects of ABS could be related to different intracellular biological pathways. (Turk J Hematol 2011; 28: 276-85)

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Section: Discussionmentioning

confidence: 99%

Section: Nf-κbmentioning

confidence: 99%

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The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Yılmaz¹,

Güleç²,

Torun³

et al. 2011

Turk J Hematol

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“…Several proteins (Homo sapiens malic enzyme 1, dynactin 5, cofilin, utrophin, mucin 16 (CD164-sialomucin- Several hypotheses will need to be raised to understand the mechanism-of-action of Ankaferd on tumor tissue [16,17]. ABS is a hemostatic agent with pleiotropic effects [14,18,21,22]. The unique protein library of Ankaferd upregulates critical transcription factors including regulators of neoplasia such as p53 [14,18].…”

Section: Discussionmentioning

confidence: 99%

Functional proteomic analysis of Ankaferd® Blood Stopper

Demiralp¹,

Haznedaroğlu²,

Akar³

2010

Turk J Hematol

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Objective: Ankaferd® Blood Stopper (ABS) comprises a standardized mixture of the plants Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum, and Urtica dioica. The basic mechanism of action for ABS is the formation of an encapsulated protein network that provides focal points for vital erythrocyte aggregation. ABSinduced protein network formation with blood cells, particularly erythrocytes, covers the primary and secondary hemostatic system without disturbing individual coagulation factors. Materials and Methods: To understand the effect mechanisms of ABS on hemostasis, a proteomic analysis using 2D gel electrophoresis and mass spectrometer was performed. Results: Proteins of plant origin in Ankaferd ® were NADP-dependent-malic enzyme, ribulose bisphosphatecarboxylase-large chain, maturase K, ATP synthase subunit-beta, ATP synthase subunit-alpha, chalcone-flavanone isomerase-1, chalcone-flavanone isomerase-2, and actin-depolymerizing factor. Furthermore, functional proteomic studies revealed that proteins resembling human peptides have been detected within Ankaferd ® , including ATP synthase, mucin-16 (CD164 sialomucin-like 2 protein), coiled-coil domain containing 141 hypothetical protein LOC283638 isoform 1, hypothetical protein LOC283638 isoform 2, dynactin 5, complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, TP synthase, H+ transporting, mitochondrial actin binding 1 isoform, LIM domain and actin binding 1 isoform a, LIM domain and actin binding 1 isoform b, spectrin alpha non erythrocytic 1, prolactin releasing hormone receptor, utrophin, tet oncogene family member 2 isoform b, protein phosphatase 1 regulatory subunit 12A, NIMA (never in mitosis gene a)-related kinase, ATP-binding cassette protein C12, Homo sapiens malic enzyme 1, mitochondrial NADP(+)-dependent malic enzyme 3, ME2 protein, nuclear factor 1 B-type, abhydrolase domain-containing protein 12B, E3 SUMO-protein ligase PIAS2, alpha-1, 2-glucosyltransferase ALG10-A, cofilin, non-muscle isoform, 18 kDa phosphoprotein, p18, actin-depolymerizing factor (ADF), twinfilin-1, ankyrin repeat and FYVE domain-containing protein 1, usherin precursor, urotensin II receptor, interleukin 4, and midkine. Conclusion: Proteomic analysis of Ankaferd ® represents a true basis for the upcoming Ankaferd ® studies focusing on its wound healing, hemostatic, anti-infective, antineoplastic, and preservative biological actions. (Turk J Hematol 2010; 27: 70-7)

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“…[19] tavşanlarda oral ABS uygulaması sonrası akut mukozal toksisite, hematotoksisite, hepatotoksisite, nefrotoksisite ve biyokimyasal toksisite geliş-mediğini göstermişlerdir. Bu yazarlar, kısa dönem ça-lışmada toksisite belirtisi olmadığını ileri sürmüşler-dir.…”

Section: Renkli şEkiller Derginin Online Sayısında Görülebilir (Wwwkunclassified

Effectiveness of Ankaferd Blood Stopper on salivary gland bleeding

Evren¹,

Çınar²,

Sarıkaya³

et al. 2012

J Kartal TR

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4 ÖzetAmaç: Bu çalışmanın amacı Ankaferd "Blood Stopper"in (ABS) tükrük bezi dokusu üzerinde kanama durdurucu etkisini incelemektir. Gereç ve Yöntem:Çalışmada 15 erişkin Wistar Albino cinsi sıçan kullanıldı. Uygun diseksiyon işlemi sonrasında sağ submandibuler glandları tam kat kesilip 0.2 cc ABS damlatıl-dı. Sol glanda da aynı kesi işlemi sonrasında salin emdirilmiş tampon ile baskı yapıldı. İki tarafın kanama süreleri ölçüldü. Bulgular: Kanama zamanı ABS uygulanan tarafta 21.9±8.9 saniye, salin emdirilmiş tampon ile baskı yapılan tarafta 89.8±33.9 saniye olarak bulundu. ABS uygulanan tarafta kanama zamanı anlamlı olarak kısa bulundu. Sonuç:Yeni bir hemostatik ajan olarak ABS'nin tükrük bezlerinde kanamayı kontrol etmek için potansiyel bir yararı olabilir. Doku üzerinde histopatolojik etkilerini incelemek, olası toksik etkilerini belirlemek için daha çok ileriye dönük kontrollü çalışmalara ihtiyaç vardır.

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Oral Systemic Administration of Ankaferd Blood Stopper Has No Short-Term Toxicity in an In Vivo Rabbit Experimental Model

Cited by 46 publications

References 14 publications

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Functional proteomic analysis of Ankaferd® Blood Stopper

Effectiveness of Ankaferd Blood Stopper on salivary gland bleeding

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