Oral testing for high‐risk human papillomavirus DNA and E6/E7 messenger RNA in healthy individuals at risk for oral infection

Rollo, Francesca; Pichi, Barbara; Benevolo, Maria; Giuliani, Massimo; Latini, Alessandra; Lorenzon, Laura; Colafigli, Manuela; Frasca, Mirko; Pellini, Raul; Cristaudo, Antonio; Donà, Maria Gabriella

doi:10.1002/cncr.32152

Cited by 8 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HIV-infected subjects also showed a lower clearance of any HPV compared with the HIV-uninfected MSM, whereas the opposite was observed for high-risk HPVs. Taken together, these data might explain the higher prevalence of any HPV in the HIV-infected subjects of the OHMAR study and the similar prevalence observed for high-risk types in the two study groups 21…”

Section: Discussionsupporting

confidence: 61%

Oral human papillomavirus infection in HIV-infected and HIV-uninfected MSM: the OHMAR prospective cohort study

et al. 2020

Self Cite

View full text Add to dashboard Cite

ObjectiveWe aimed to assess incidence and clearance of oral human papillomavirus (HPV) infection and the respective risk factors in HIV-infected and uninfected men who have sex with men (MSM).MethodsOral rinse and gargles were collected semiannually from 244 MSM (103, 42.2% HIV-infected). HPV-DNA testing was performed with the Linear Array HPV Genotyping test. A Markov model was used for estimation of incidence, clearance and risk factor analysis.ResultsIncidence rates for any HPV were 21.2 and 15.0×1000 person-months in HIV-infected and uninfected MSM, respectively. The respective figures for high-risk HPVs were 10.7 and 6.5×1000 person-months. The clearance rate was 4–12 times higher than the respective incidence rate. HIV-infected MSM with >95 lifetime oral sex partners showed increased incidence of any HPV (adjusted HR, aHR: 8.46, 95% CI 1.89 to 37.92). Condomless oral sex appeared the strongest predictor for incident infection by high-risk HPVs in this group (aHR: 13.40, 95% CI 2.55 to 70.53). Those aged >46 years (aHR: 0.30, 95% CI 0.12 to 0.74) and those with nadir CD4+ T count of <200 cells/mm3 (aHR: 0.14, 95% CI 0.03 to 0.75) displayed a significantly reduced clearance of any and high-risk HPVs, respectively. HIV-uninfected MSM aged >46 years had increased risk of acquiring any HPV (aHR: 3.70, 95% CI 1.30 to 10.52) and high-risk HPV (aHR: 5.33, 95% CI 1.06 to 26.68). Any HPV clearance declined in those with more than six recent oral sex partners (aHR: 0.18, 95% CI 0.05 to 0.65).ConclusionsAcquisition of oral HPV infection in MSM seems to occur rarely, whereas clearance seems to be a frequent event. Oral HPV natural history in these at-risk subjects is differently influenced by age and sex behaviour, depending on HIV status.

show abstract

Section: Discussionsupporting

confidence: 61%

Oral human papillomavirus infection in HIV-infected and HIV-uninfected MSM: the OHMAR prospective cohort study

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Of these, 117 were HIV-infected subjects (37.7%), for the most part on cART (110, 94.0%), with a median number of nadir and a current CD4+ T-cell count of 300 (IQR: 202-403) and 636 cells/mm3 (IQR: 473-812), respectively. Sociodemographic and behavioral characteristics of the participants in the OHMAR study were detailed in a previous report [32]. To summarize, HIV-infected subjects were significantly older (median age: 43 vs. 39 years, p = 0.005) and reported a significantly lower number of recent oral sex partners (median of two vs. four, p = 0.003) than the HIV-uninfected counterparts.…”

Section: Study Populationmentioning

confidence: 94%

Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study

Gheit

Rollo

Brancaccio

et al. 2020

Viruses

Self Cite

View full text Add to dashboard Cite

Both mucosal and cutaneous Human Papillomaviruses (HPVs) can be detected in the oral cavity, but investigations regarding the epidemiology of cutaneous HPVs at this site are scarce. We assessed mucosal (alpha) and cutaneous (beta and gamma) HPV infection in oral samples of HIV-infected and uninfected men who have sex with men (MSM). Oral rinse-and-gargles were collected from 310 MSM. Alpha HPVs were detected using the Linear Array, whereas beta and gamma HPVs were detected using multiplex PCR and Luminex technology. An amplicon-based next-generation sequencing (NGS) protocol was applied to a subset of samples collected from 30 HIV-uninfected and 30 HIV-infected MSM. Beta HPVs were significantly more common than alpha types (53.8% vs. 23.9% for HIV-infected subjects, p < 0.0001; 50.3% vs. 17.1% for HIV-uninfected subjects, p < 0.0001). Gamma HPVs were also frequently detected (30.8% and 25.9% in HIV-infected and uninfected MSM, respectively). NGS produced 2,620,725 reads representative of 146 known HPVs (16 alpha-PVs, 53 beta-PVs, 76 gamma-PVs, one unclassified) and eight putative new HPVs, taxonomically assigned to the beta genus. The oral cavity contains a wide spectrum of HPVs, with beta types representing the predominant genus. The prevalence of beta and gamma HPVs is high even in immunorestored HIV-infected individuals. NGS confirmed the abundance of cutaneous HPVs and identified some putative novel beta HPVs. This study confirms that cutaneous HPVs are frequently present at mucosal sites and highlights that their pathological role deserves further investigation since it may not be limited to skin lesions.

show abstract

“…The large majority of these subjects were on combined antiretroviral therapy (cART) (110, 94.0%). With regard to sociodemographic features and lifestyle, the OHMAR study population has been previously described [15]. Briefly, HIV-infected and HIV-uninfected MSM differed significantly with respect to age (median age: 43 vs. 39 years) and number of recent oral sex partners (median of two vs. four).…”

Section: Study Populationmentioning

confidence: 99%

“…Thus, additional studies are needed to thoroughly explore the determinants of infection with the different HPV genera. Compared with the general population, prevalence of oral infection by mucosal HPVs is higher in men who have sex with men (MSM), particularly in those living with HIV-1 infection [8,[11][12][13][14][15]. In a previous study, we showed that beta and gamma HPVs are abundantly present in oral specimens from MSM, and that oral infection by beta HPVs is significantly more common than that caused by alpha types, irrespective of HIV status [6].…”

Section: Introductionmentioning

confidence: 99%

Predictors of Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in HIV-Infected and Uninfected Men Who Have Sex with Men of the OHMAR Study

Giuliani

Gheit

Rollo

et al. 2021

JCM

Self Cite

View full text Add to dashboard Cite

Mucosal Human Papillomaviruses (HPVs) play a role in the development of a subset of head and neck cancers. Cutaneous HPVs are abundantly present in the oral cavity. The determinants of these infections have not been extensively investigated. We assessed the correlates of oral infection by alpha and beta and/or gamma HPVs in HIV-infected and uninfected men who have sex with men (MSM). Oral rinse-and-gargles were collected with a mouthwash. Alpha and beta/gamma HPVs were detected using the Linear Array HPV genotyping test and a multiplex PCR combined with Luminex technology, respectively. Multiple logistic regression was performed to identify independent predictors of oral HPV infection. Overall, 193 HIV-uninfected and 117 HIV-infected MSM were enrolled. Among HIV-infected MSM, the only determinant of alpha HPV infection was the number of lifetime oral sex partners (AOR: 8.26, 95% CI: 2.26–30.16). The strongest determinant of beta/gamma HPV infection was represented by practicing condomless receptive oral sex (AOR: 10.76, 95% CI: 1.56–74.17). Age was independently associated with alpha HPV infection in HIV-uninfected MSM. Beta/gamma HPV infection was not associated with sexual behavior in these subjects. In conclusion, predictors of oral infection differ between HIV-infected and uninfected MSM, as well as between alpha and beta/gamma HPVs.

show abstract

Oral testing for high‐risk human papillomavirus DNA and E6/E7 messenger RNA in healthy individuals at risk for oral infection

Cited by 8 publications

References 28 publications

Oral human papillomavirus infection in HIV-infected and HIV-uninfected MSM: the OHMAR prospective cohort study

Oral human papillomavirus infection in HIV-infected and HIV-uninfected MSM: the OHMAR prospective cohort study

Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study

Predictors of Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in HIV-Infected and Uninfected Men Who Have Sex with Men of the OHMAR Study

Contact Info

Product

Resources

About