Oral vaccination against mycoplasmal pneumonia of swine using a live Erysipelothrix rhusiopathiae vaccine strain as a vector

Immunostimulatory Effects of Recombinant Erysipelothrix rhusiopathiae Expressing Porcine Interleukin-18 in Mice and Pigs

“…We previously showed that unlike an E. rhusiopathiae YS-1-based vaccine, a Koganei 65-0.15-based vaccine could colonize the tonsils of pigs who received the vaccine via the oral route and could induce protective immunity against erysipelas and mycoplasmal pneumonia (21). Therefore, in the pig experiment, we used KO/IL-18, a Koganei 65-0.15 derivative expressing poIL-18.…”

“…Thus far, we have assessed the potential use of attenuated strains of E. rhusiopathiae as vectors for delivering the P97 adhesin antigen of Mycoplasma hyopneumoniae, the etiological agent of mycoplasmal or enzootic pneumonia of swine (21,27,28). We used the YS-1 strain (26), an acapsular attenuated mutant of E. rhusiopathiae, and the Koganei 65-0.15 strain, the live swine erysipelas vaccine in Japan, and engineered these strains to express a recombinant protein containing the carboxyl-terminal portion of the P97 adhesin on the cell surface.…”

“…We used the YS-1 strain (26), an acapsular attenuated mutant of E. rhusiopathiae, and the Koganei 65-0.15 strain, the live swine erysipelas vaccine in Japan, and engineered these strains to express a recombinant protein containing the carboxyl-terminal portion of the P97 adhesin on the cell surface. We examined the vaccine efficacy of these strains against erysipelas and mycoplasmal pneumonia by immunizing pigs intranasally or orally, and we demonstrated that E. rhusiopathiae is a promising vaccine vector for delivering foreign antigens to the immune system of pigs (21,27,28).…”

A recombinant chimera comprising the R1 and R2 repeat regions of M. hyopneumoniae P97 and the N-terminal region of A. pleuropneumoniaeApxIII elicits immune responses

“…Mice that orally ingested Salmonella typhimurium harboring eukaryotic or prokaryotic expression vector encoding the R1 region showed R1-specific Th1 responses [28]. Intranasal and oral immunization of Erysipelothrix rhusiopathiae strains expressing the C -terminal portion of P97, including the R1 and R2 regions, to pigs reduced the severity of pneumonic lung lesions caused by M. hyopneumoniae infection [29,30]. Adenovirus expressing the C -terminal portion of P97, containing the R1 and R2 regions, also induced protective P97-specific humoral and cellular immune responses in pigs [31].…”

AttenuatedActinobacillus pleuropneumoniaeas a bacterial vector for expression ofMycoplasma hyopneumoniaeP36 gene

“…Live vaccines with foreign immunogenes elicit cellular immune responses and mucosal immunoglobulin A antibody. Live vaccines based on bacterial vectors, such as Salmonella typhimurium 18–21 and Erysipelothrix rhusiopathiae 22–24, efficiently induce protective immunity. Pigs that survive from natural infection with A. pleuropneumoniae is completely protected against homologous infection and partially cross‐protected from infection with heterologous serotypes of A. pleuropneumoniae 8–14, 25, with the pigs producing antibodies against the virulence factors of the bacteria.…”