Oral vaccination of fish againstVibrio anguillarumusing alginate microparticles

Joosten, Peter; Tiemersma, Edine; Threels, A.; Dhieux-Caumartin, C.; Rombout, J.H.W.M.

doi:10.1006/fsim.1997.0100

Cited by 114 publications

(76 citation statements)

References 31 publications

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“…The existence of tetrameric IgM in the skin mucus of teleost has been well documented (5,7,8,10,11,(13)(14)(15)(16)(17). However, the transport system associated with tetrameric IgM in this tissue has yet to be defined.…”

Section: Discussionmentioning

confidence: 99%

A Teleost Polymeric Ig Receptor Exhibiting Two Ig-Like Domains Transports Tetrameric IgM into the Skin

Hamuro

Suetake

Saha³

et al. 2007

The Journal of Immunology

130

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The skin mucus IgM is an important molecule in the mucosal immune system of teleost skin. However, the transport mechanism associated with this molecule has yet to be clarified. In this study, we isolated a gene encoding a polymeric Ig receptor (pIgR) from a species of teleost fish, Takifugu rubripes (fugu). This gene is known to be an Ig transporter in the intestine of mammals. Our studies further demonstrated that fugu pIgR was expressed in the skin and that a fragment of pIgR bound to tetrameric IgM in the skin mucus. These results indicate that the skin pIgR transports tetrameric IgM into the skin mucus. The fugu pIgR exhibits a unique structure containing only two Ig-like domains corresponding to domain 1 and domain 4/5 of mammalian pIgR. This structure was sufficient for successful binding to tetrameric IgM. Teleost skin thus adopts the same Ig transport system as mammalian intestine via a unique pIgR.

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Section: Discussionmentioning

confidence: 99%

A Teleost Polymeric Ig Receptor Exhibiting Two Ig-Like Domains Transports Tetrameric IgM into the Skin

Hamuro

Suetake

Saha³

et al. 2007

The Journal of Immunology

130

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“…In fact, antigens are found carp enterocytes 16 hours after administering the vaccine, while they are found in the enterocytes of rainbow trout 24 hours after administration. Finally, the response of immunoreactive macrophages is only found at 36 hours postadministration (Joosten et al, 1997).…”

Section: Bacterial Antigens Encapsulated In Alginatementioning

confidence: 94%

“…Microparticles of bacteria such as Vibrio Anguillarum administered orally increase the number of antibodies in the mucus of the gut and gills and very little in serum. In contrast, antibodies increased in serum, head kidney, blood, but not in the gut and gills in carp vaccinated and challenged intramuscularly (Joosten et al, 1997). Although in the case of carp the vaccination with microparticles is capable alone of increasing the number of antibodies in serum, with rainbow trout a booster is required to do the same.…”

Section: Bacterial Antigens Encapsulated In Alginatementioning

confidence: 95%

“…One of the characteristics of alginate microparticles that makes them useful for delivering drugs is their resistance to acid pH, which impedes the release of the antigen in the stomach of the fi sh (pH 2-4) and favors their its release in the foregut or hindgut (pH 7 and 8.3, respectively) (Joosten et al, 1997;Leal et al, 2010;Rodrigues et al, 2006;Salinas et al, 2011). Microparticles from 10 to 50 μm can be effi cient for vaccinating, although it has been shown that the smaller the size, the greater the effi ciency of incorporation (Rodrigues et al, 2006;Romalde et al, 2004).…”

Section: Alginatementioning

confidence: 99%

“…The effi ciency of this response depends on the immunization route, given that oral and anal administration in fi sh activates GALT, which presents greater effi ciency in some species. Immersion activates SALT and GIALT, while injection generally does not activate any of these (Salinas et al, 2011;Joosten et al, 1997;Rombout et al, 1989). Parallel to this, it has been shown that the enterocytes in the hindgut are capable of transporting antigens and that in the lamellae and epithelial cells there are populations of macrophages and lymphoid cells that can participate in presenting antigens in the intestine of the fi sh (Doggett & Harris 1991;Georgopoulou & Vernier 1986;Rombout et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines

2013

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Aquaculture has become an important economic sector worldwide, but is faced with an ongoing threat from infectious diseases. Vaccination plays a critical role in protecting commercially raised fi sh from bacterial, viral and parasitic diseases. However, the production of eff ective vaccines is limited by the scarcity of knowledge about the immune system of fi sh. Improving vaccines implies using antigens, adjuvants and employing methods of administration that are more eff ective and less harmful to the fi sh. In this context, in recent year there have studies of methods of encapsulating antigens in matrices of diff erent types to apply in fi sh vaccines. This work reviews the new methods to improve fi sh vaccines by encapsulating them in polymers and polysaccharides.

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2012

Fish Pathology

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Oral vaccination of fish againstVibrio anguillarumusing alginate microparticles

Cited by 114 publications

References 31 publications

A Teleost Polymeric Ig Receptor Exhibiting Two Ig-Like Domains Transports Tetrameric IgM into the Skin

A Teleost Polymeric Ig Receptor Exhibiting Two Ig-Like Domains Transports Tetrameric IgM into the Skin

Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines

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