Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study

Abstract

Introduction: Tobacco smoke is associated with oxidative and inflammatory pathways, increasing the risk of chronic-degenerative diseases. Our goal was to evaluate the effects of acute “Pera” and “Moro” orange juice consumption on inflammatory processes and oxidative stress in microRNA (miRNA) expression in plasma from healthy smokers.Methods: This was a randomized crossover study that included healthy smokers over 18 years old. Blood samples were collected before and 11 h after beverage ingestion. Participants were instructed to drink 400 mL of Pera orange juice (Citrus sinensis), Moro orange juice (Citrus sinensis L. Osbeck), or water. Each subject drank the beverages in a 3-way crossover study design. Inflammatory and oxidative stress biomarkers and circulating miRNA expression profiles were determined. The subjects maintained their usual tobacco exposure during the experiment.Results: We included 18 individuals (12 men and 6 women), with 37.0 ± 12.0 years old. All subjects received the 3 interventions. Increased expression of circulating miRNAs (miR-150-5p, miR-25-3p, and miR-451a) was verified after cigarette smoking, which were attenuated after intake of both types of orange juice. There was no difference regarding serum levels of TNF-α, IL-6, MMP-9, and C-reactive protein. Despite the increased activity of serum superoxide dismutase and glutathione peroxidase after “Pera” or “Moro” orange juice intake, respectively, no changes in lipid hydroperoxide levels were detected.Conclusion: Tobaccos smokers showed increased expression of miR-150-5p, miR-25-3p, and miR-451a was noted, and attenuated by orange juice intake. miRNAs were predicted to regulate 244 target genes with roles in oxidative stress, PI3K-Akt, and MAPK signaling, which are pathways frequently involved in smoking-related cardiovascular diseases and cancer.

Plasma proteome profiling reveals molecular mechanisms underlying the effects of daily consumption of ‘Bahia’ and ‘Cara Cara’ orange juices

“…These 5 miRNAs regulate 52 proteins identified as differently expressed in our study. Other 6 miRNAs (miR-150-5p, miR-375, miR-130b-3p, miR-25-3p, miR-424-5p, and miR-144-3p) were previously reported [68][69][70] as modulated by OJ consumption, which directly regulate 21 differentially expressed proteins in our study. Taken together, bioinformatic analyses evidenced the potential effect of OJ consumption on several metabolic processes and shed light on four pathways (focal adhesion, HIF-1 signaling pathway, phagosome, and PI3K-Akt signaling pathway), which were identified as containing the higher numbers of overlapped proteins considering both datasets.…”

Plasma Extracellular MicroRNAs Associated With Cardiovascular Disease Risk Factors in Middle‐Aged and Older Adults

“…found that plasma levels of circulating miR‐150‐5p were higher following tobacco intake via cigarette smoking. 105 Bioinformatic analysis was used to identify oxidative stress, and the ErbB signaling pathway was found to be among the enriched pathways regulated by genes targeted by miRNAs. This pathway has previously been implicated in numerous smoking‐related cardiovascular diseases due.…”

Blood orange juice intake changes specific bacteria of gut microbiota associated with cardiometabolic biomarkers

“…Several recent studies have investigated the benefits of blood orange juice intake, especially of the Moro variety ( Dorna et al, 2021 ; Nishioka et al, 2021 ; Ribeiro et al, 2021 ; Briskey et al, 2022 ; Santana et al, 2022 ; Capetini et al, 2023 ). However, the effects of MOJ on the gut microbiota profiles have not been investigated in detail.…”

“…Recent studies have identified novel polymorphisms ( Fraga et al, 2022 ) and microRNAs ( Dorna et al, 2021 ; Quintanilha et al, 2022 ; Capetini et al, 2023 ), which regulate cellular processes and mechanisms that are associated with the benefits of orange juice intake. This is one of the few studies that has evaluated the effects of MOJ on the gut microbiota composition and is the first study to report the correlation between the gut microbiota composition and the cardiometabolic biomarkers.…”