1Due to the scarcity of reliable and highly resolved moisture proxies covering much of the 2 Holocene, there has been increased interest in the study of living and subfossil peatland trees 3 sensitive to gradual and extreme changes in hydrology, precipitation, and related environmental 4 processes. Peatland development and the associated carbon accumulation, which are strongly 5 influenced by hydrological fluctuations, are also of prime importance as peatlands represent long-6 term sinks of atmospheric carbon. Improved knowledge of peatland development and soil 7 moisture variability during the Holocene is therefore essential to our understanding of long-term 8 hydroclimate changes, the terrestrial carbon cycle, and to enable more robust predictions of 9 peatland response to future climate changes. 10Here, we review the existing mid-to late Holocene peatland tree-ring chronologies that 11 have been used to study climate variability on (sub-)annual to centennial scales with a primary 12 focus on northern Europe. Since the 1970s, absolutely dated tree-ring chronologies covering 13 substantial parts of the Holocene have been developed from excavated remains of oak (Quercus 14 spp.) and pine (Pinus sylvestris L.). The annual tree-ring patterns of these trees are often 15 characterized by periods of depressed growth reflecting annual to decadal hydroclimatic changes. 16In addition, changes in the spatio-temporal distribution of trees throughout the Holocene are often 17 found to reflect decadal to centennial climate and hydrological changes. Moreover, synchronicity 18 between tree-ring chronologies and tree-population dynamics over larger geographical areas 19 show periods of coherent regional climate forcing, especially during the mid-Holocene. 20This review (i) provides an overview of pioneering and recent studies presenting tree-ring 21 chronologies developed from subfossil peatland trees, and (ii) presents recent developments in the 22 fields of dendroecology (i.e. the response of tree growth and changes in vitality as a result of 23 changes in climatic variables) and dendroclimatology (i.e. the reconstruction of climate 24 4 fluctuations based on tree-ring analyses) in peatland regions. Moreover, we (iii) use long-term 25 climate reconstructions based on alternative proxies for comparison, and (iv) present different 26 ways to analyse tree-ring records to generate novel information on annual to centennial 27 timescales. This analysis is based on an unprecedented network of tree-ring chronologies from 28 Denmark, Finland, Germany, Great Britain, Ireland, Lithuania, the Netherlands, Poland, Sweden, 29and Canada, as well as a wealth of old and previously (un) published literature from Scandinavia 30 and Germany, which has not been accessible to a wider audience in the past due to inaccessibility 31 or linguistic barriers. Finally, a map of possible hotspots for the assessment of continuous 32 peatland-tree studies is presented, along with suggestions for new research directions in the field. 33 34