Orcokinin neuropeptides regulate reproduction in the fruit fly, Drosophila melanogaster

Silva, Valeria; Palacios-Muñoz, Angelina; Volonté, Mariano; Frenkel, Lia; Ewer, John; Ons, Sheila

doi:10.1016/j.ibmb.2021.103676

Cited by 14 publications

(15 citation statements)

References 32 publications

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“…Holometabola and Hemimetabola, our results suggest high functional conservation in the neuropeptides that regulate ecdysis. The only exception is OKA, which seems to be essential for successful ecdysis in R. prolixus (present results, (Wulff et al, 2017(Wulff et al, , 2018 but not in D. melanogaster (Silva et al, 2021). However, the involvement of OKA in ecdysis could be restricted to Hemiptera or Heteroptera, given that this phenotype was not observed in B. germanica (Ons et al, 2015).…”

contrasting

confidence: 56%

“…Interestingly, a recent survey demonstrated that the expression profiles of ecdysis-related neuropeptides correlate positively with each other in a wide range of species (Zieger et al, 2021); the results presented here confirm this tendency for R. prolixus . Given that the down-regulation of OKA expression doesn’t affect ecdysis in D. melanogaster (Silva et al, 2021) nor in Blattella germanica (Ons et al, 2015), positive feedback seems to be not conserved among insects. Further experiments with species from different Orders will be interesting to infer the role of OKA signalling throughout the evolution of ecdysis regulation.…”

Section: Discussionmentioning

confidence: 99%

“…A few studies indicated that ETH and CCAP are involved in ecdysis regulation in hemimetabolous insects (Lee et al, 2013;Lenaerts et al, 2017;Shi et al, 2022;Verbakel et al, 2021), suggesting a conserved regulatory network. Conversely, Orcokinin isoform A (OKA) neuropeptides play a central role in the regulation of ecdysis in Rhodnius prolixus (Wulff et al, 2017(Wulff et al, , 2018 but not in D. melanogaster (Silva et al, 2021), pointing to differences in ecdysis regulation between Holo and Hemimetabola.…”

Section: Introductionmentioning

confidence: 99%

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The role of neuropeptides in regulating ecdysis and reproduction in the hemimetabolous insect Rhodnius prolixus

Sterkel

Volonté

Albornoz

et al. 2022

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In ecdysozoan animals, moulting entails the production of a new exoskele-ton and the shedding of the old one during ecdysis. It is induced by a pulse of ecdysone that regulates the expression of different hormonal receptors and acti-vates a peptide-mediated signalling cascade. In Holometabola, the peptidergic cascade regulating ecdysis has been well described. However, very little function-al information regarding the neuroendocrine regulation of ecdysis is available for Hemimetabola, which displays an incomplete metamorphosis. Here, we studied neuropeptides related to ecdysis regulation in the hemi-metabolous insect Rhodnius prolixus. The RNA interference-mediated reduction of ETH expression in fourth instar nymphs resulted in lethality at the expected time of ecdysis, thereby showing its crucial role in this process. Furthermore, the results revealed the involvement of ETH in the regulation of reproductive fitness. Different from holometabolous, the knockdown of ETH in adult females led to failures in egg hatching without affecting the oviposition. Most of the first instar nymphs hatched from the eggs laid by females injected with dsEH, dsCCAP and dsOKA died at the expected time of ecdysis, indicating the crucial involvement of these genes for post-embryonic development. No phenotypes were observed up-on CZ knockdown in nymphs or adult females. The conservation of the role of these neuropeptides in regulating ecdysis and reproduction throughout the class Insecta is discussed.

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contrasting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The role of neuropeptides in regulating ecdysis and reproduction in the hemimetabolous insect Rhodnius prolixus

Sterkel

Volonté

Albornoz

et al. 2022

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“…Interestingly, a recent survey demonstrated that the expression profiles of ecdysis-related neuropeptides correlate positively with each other in a wide range of species (Zieger et al, 2021); the results presented here confirm this tendency for R. prolixus. Given that the downregulation of OKA expression doesn't affect ecdysis in D. melanogaster (Silva et al, 2021) nor in Blattella germanica (Ons et al, 2015), positive feedback seems to be not conserved among insects. Further experiments with species from different Orders will be interesting to infer the role of OKA signalling throughout the evolution of ecdysis regulation.…”

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

The role of neuropeptides in regulating ecdysis and reproduction in the hemimetabolous insect Rhodnius prolixus

Sterkel

Volonté

Albornoz

et al. 2022

Journal of Experimental Biology

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In ecdysozoan animals, moulting entails the production of a new exoskeleton and shedding the old one during ecdysis. It is induced by a pulse of ecdysone that regulates the expression of different hormonal receptors and activates a peptide-mediated signalling cascade. In Holometabola, the peptidergic cascade regulating ecdysis has been well described. However, very little functional information regarding the neuroendocrine regulation of ecdysis is available for Hemimetabola, which displays an incomplete metamorphosis. We use Rhodnius prolixus as a convenient experimental model to test two hypotheses: (a) the role of neuropeptides that regulate ecdysis in Holometabola is conserved in hemimetabolous insects; (b) the neuropeptides regulating ecdysis play a role in the regulation of female reproduction during the adult stage. The RNA interference-mediated reduction of ETH expression in fourth-instar nymphs resulted in lethality at the expected time of ecdysis. Unlike in holometabolous insects, the knockdown of ETH and OKA did not affect oviposition in adult females, pointing to a different endocrine regulation of ovary maturation. However, ETH knockdown prevented egg hatching. The blockage of egg hatching appears to be a consequence of embryonic ecdysis failure. Most of the first-instar nymphs hatched from the eggs laid by females injected with dsEH, dsCCAP and dsOKA died at the expected time of ecdysis, indicating the crucial involvement of these genes in post-embryonic development. No phenotypes were observed upon CZ knockdown in nymphs or adult females. The results are relevant for evolutionary entomology and could reveal targets for neuropeptide-based pest control tools.

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Identification of neuropeptides and their G protein‐coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae)

Huang,

Dong,

Yang

et al. 2024

Arch Insect Biochem Physiol

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The predatory stink bug Arma custos has been selected as an effective biological control agent and has been successfully massly bred and released into fields for the control of a diverse insect pests. As a zoophytophagous generalist, A. custos relies on a complex neuropeptide signaling system to prey on distinct food and adapt to different environments. However, information about neuropeptide signaling genes in A. custos has not been reported to date. In the present study, a total of 57 neuropeptide precursor transcripts and 41 potential neuropeptide G protein‐coupled receptor (GPCR) transcripts were found mainly using our sequenced transcriptome data. Furthermore, a number of neuropeptides and their GPCR receptors that were enriched in guts and salivary glands of A. custos were identified, which might play critical roles in feeding and digestion. Our study provides basic information for an in‐depth understanding of biological and ecological characteristics of the predatory bug and would aid in the development of better pest management strategies based on the effective utilization and protection of beneficial natural enemies.

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Orcokinin neuropeptides regulate reproduction in the fruit fly, Drosophila melanogaster

Cited by 14 publications

References 32 publications

The role of neuropeptides in regulating ecdysis and reproduction in the hemimetabolous insect Rhodnius prolixus

The role of neuropeptides in regulating ecdysis and reproduction in the hemimetabolous insect Rhodnius prolixus

The role of neuropeptides in regulating ecdysis and reproduction in the hemimetabolous insect Rhodnius prolixus

Identification of neuropeptides and their G protein‐coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae)

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