Ordered Conformation of Succinoglycan in Aqueous Sodium Chloride

Abstract

Succinoglycan samples ranging in weight-average molecular weight from 1.0 x 10(5) to 8.7 x 10(6) (in 0.1 M aqueous NaCl at 25 degrees C), prepared by ultrasonication of a native sample (Rheozan), followed by fractionation, were investigated by static light scattering, sedimentation equilibrium, and viscometry in 0.1 M aqueous NaCl at 25 degrees C where the polysaccharide assumes a certain ordered (helical) conformation. The measured radii of gyration and intrinsic viscosities showed the polysaccharide to behave like a semirigid chain in the aqueous salt. Their analysis based on the unperturbed wormlike chain yielded about 1500 nm-1 and 50 nm for the linear mass density and the persistence length, respectively. The former value was almost twice that expected for the single succinoglycan molecule, and thus it was concluded that the predominant molecular species of succinoglycan present in the aqueous salt is a double helix or an aggregate composed of paired single helices.

Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp

“…[4], commonly, the values of M L and q respectively range from 400–2,200 nm −1 and 3–200 nm. The M L and q values of longan polysaccharides were relatively close to those of semi-flexible curdlan (890 nm −1 , 6.8 nm) [30] and semi-stiff sulfated glucan from Poria cocos (1060 nm −1 , 13.1 nm) [31], larger than those of random-coil polysaccharides from Pleurotus tuberregium (408 nm −1 , 3.1 nm) [32] and Ganoderma tsugae (832 nm −1 , 4.2 nm) [26], and much smaller than those of triple helical lentinan (2,240 nm −1 , 100 nm) [11] and single helical succinoglycan (1,500 nm −1 , 50 nm) [33]. It was implied thus that longan polysaccharides in the M w range of 1.459 × 10 4 –5.282 × 10 6 g/mol existed as semi-flexible chains.…”

Variable chain conformations of renatured β‐glucan in dimethylsulfoxide/water mixture

“…However, single chain of beta‐glucan did not show any melting signal in DSC curves. Moreover, the transition temperature of triple helixes including SPG, scleroglucan and LNT is higher than 130 o C,35 while the double helical beta‐glucan succinoglycan was disrupted into single chains at 60 o C 48. Meanwhile, the aggregates can not combine with poly(dA) due to the occupied hydrogen bonding sites, if the aggregates exist as the major species in solution, changes for the CD spectra will not be observed.…”

Chemical and rheological properties of bacterial succinoglycan with distinct structural characteristics

“…The characteristics of succinoglycans in aqueous solutions have been analyzed by several researchers (Balnois et al, 2000;Gravanis, Milas, Rinaudo, & Tinland, 1987;Kaneda & Yanaki, 2002;Kido, Nakanishi, Norisuye, Kaneda, & Yanaki, 2001). It shows reversible pseudoplastic behavior in solution (Gravanis, Milas, Rinaudo, & Clarkesturman, 1990;Ridout et al, 1997).…”