Orenia salinaria sp. nov., a fermentative bacterium isolated from anaerobic sediments of Mediterranean salterns.

Mouné, Sophie; Eatock, Claire; Matheron, Robert; Willison, John C.; Hirschler, A; Herbert, R. A.; Cartigny, Pierre

doi:10.1099/00207713-50-2-721

Cited by 41 publications

(26 citation statements)

References 30 publications

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“…The natural habitats for these known Orenia populations reflect their capacity to tolerate a broad range of temperature and salt concentrations. Previous physiological characterization of these Orenia species, however, has focused on the fermentation of saccharides or polysaccharides (e.g., chitin) (38,49,59,60). As with other Orenia populations, strain Z6 exhibits rod-shaped morphology, low GϩC content, tolerance of comparable ranges of pH, temperature, and salinity, as well as active fermentation of sugars (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…2) (38,49,59,60). A screen of the NCBI and RDP reference databases (32) also shows that closely related sequences or phylotypes are not abundant.…”

Section: Discussionmentioning

confidence: 99%

“…All previous Orenia species (O. marismortui, O. salinaria, O. sivashensis, and O. chitinitropha) (Fig. 2) were isolated from saline or hypersaline salterns or lakes (38,49,59,60). The natural habitats for these known Orenia populations reflect their capacity to tolerate a broad range of temperature and salt concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…Susceptibility to antibiotics was tested by amending the iron-reducing cultures with one of the following antibiotics: 40 g/ml ampicillin, anisomycin, tetracycline, erythromycin, or kanamycin plus 20 g/ml chloramphenicol (38).…”

Section: Mediamentioning

confidence: 99%

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Orenia metallireducens sp. nov. Strain Z6, a Novel Metal-Reducing Member of the Phylum Firmicutes from the Deep Subsurface

Dong

Sanford

Boyanov

et al. 2016

Appl Environ Microbiol

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A novel halophilic and metal-reducing bacterium, Orenia metallireducens strain Z6, was isolated from briny groundwater extracted from a 2.02 km-deep borehole in the Illinois Basin, IL. This organism shared 96% 16S rRNA gene similarity with Orenia marismortui but demonstrated physiological properties previously unknown for this genus. In addition to exhibiting a fermentative metabolism typical of the genus Orenia, strain Z6 reduces various metal oxides [Fe(III), Mn(IV), Co(III), and Cr(VI)], using H 2 as the electron donor. Strain Z6 actively reduced ferrihydrite over broad ranges of pH (6 to 9.6), salinity (0.4 to 3.5 M NaCl), and temperature (20 to 60°C). At pH 6.5, strain Z6 also reduced more crystalline iron oxides, such as lepidocrocite (␥- IMPORTANCEA novel iron-reducing species, Orenia metallireducens sp. nov., strain Z6, was isolated from groundwater collected from a geological formation located 2.02 km below land surface in the Illinois Basin, USA. Phylogenetic, physiologic, and genomic analyses of strain Z6 found it to have unique properties for iron reducers, including (i) active microbial iron-reducing capacity under broad ranges of temperatures (20 to 60°C), pHs (6 to 9.6), and salinities (0.4 to 3.5 M NaCl), (ii) lack of c-type cytochromes typically affiliated with iron reduction in Geobacter and Shewanella species, and (iii) being the only member of the Halanaerobiales capable of reducing crystalline goethite and hematite. This study expands the scope of phylogenetic affiliations, metabolic capacities, and catalytic mechanisms for iron-reducing microbes.T he reduction of ferric [Fe(III)-bearing] minerals by microorganisms is widespread in both terrestrial and marine environments (1, 2) and is potentially one of the earliest forms of metabolism (3, 4). Due to the abundance of ferric minerals in the Earth's crust and the ubiquity of dissimilatory metal-reducing bacteria (DMRB), Fe(III) reduction is of global environmental significance, particularly in the subsurface. A phylogenetic variety of organisms has been reported for the capacity to reduce iron in a dissimilatory manner (1, 5). Dissimilatory iron reduction can be classified into two major groups. Many of the iron-reducing organisms (e.g., fermentative iron reducers) use Fe(III) as a minor side reaction in their metabolism but do not appear to conserve energy to support growth from this electron transfer. In comparison, the respiratory iron reducers conserve energy to support growth from Fe(III) via an electron transfer chain (1,5). DMRB strongly influence the biogeochemical cycling of metals and mineralization of organic matter in aquatic and sedimentary environments (1,(6)(7)(8). In addition, DMRB play a key role in the bioremediation of hazardous contaminants, such as heavy metals, radionuclides, and hydrocarbons (9). DMRB capable of reducing ferric minerals have previously been isolated from gold mines, petroleum reservoirs, and other

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Section: Discussionmentioning

confidence: 99%

“…2) (38,49,59,60). A screen of the NCBI and RDP reference databases (32) also shows that closely related sequences or phylotypes are not abundant.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Mediamentioning

confidence: 99%

See 2 more Smart Citations

Orenia metallireducens sp. nov. Strain Z6, a Novel Metal-Reducing Member of the Phylum Firmicutes from the Deep Subsurface

Dong

Sanford

Boyanov

et al. 2016

Appl Environ Microbiol

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“…Isolation of genomic DNA, amplification of 16S rDNA by PCR, sequencing and phylogenetic analysis were all carried out as described previously (Mouné et al, 1999(Mouné et al, , 2000. The accession numbers of all 16S rDNA sequences used in this study are given in Fig.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of spirilloid purple phototrophic bacteria forming red layers in microbial mats of Mediterranean salterns: description of Halorhodospira neutriphila sp. nov. and emendation of the genus Halorhodospira

Hirschler-Réa

Matheron

Riffaud

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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Microbial mats developing in the hypersaline lagoons of a commercial saltern in the Salin-de-Giraud (Rhô ne delta) were found to contain a red layer fully dominated by spirilloid phototrophic purple bacteria underlying a cyanobacterial layer. From this layer four strains of spirilloid purple bacteria were isolated, all of which were extremely halophilic. All strains were isolated by using the same medium under halophilic photolithoheterotrophic conditions. One of them, strain SG 3105 was a purple non-sulfur bacterial strain closely related to Rhodovibrio sodomensis with a 16S rDNA sequence similarity of 98?8 %. The three other isolated strains, SG 3301 T , SG 3302 and SG 3304, were purple sulfur bacteria and were found to be very similar. The cells were motile by a polar tuft of flagella. Photosynthetic intracytoplasmic membranes of the lamellar stack type contained BChl a and spirilloxanthin as the major carotenoid. Phototrophic growth with sulfide as electron donor was poor; globules of elemental sulfur were present outside the cells. In the presence of sulfide and CO 2 good growth occurred with organic substrates. Optimum growth occurred in the presence of 9-12 % (w/v) NaCl at neutral pH (optimal pH 6?8-7) and at 30-35˚C. The DNA base composition of strains SG 3301 T and SG 3304 were 74?5 and 74?1 mol% G+C, respectively.According to the 16S rDNA sequences, strains SG 3301 T and SG 3304 belonged to the genus Halorhodospira, but they were sufficiently separated morphologically, physiologically and genetically from other recognized Halorhodospira species to be described as a new species of the genus. They are, therefore, described as Halorhodospira neutriphila sp. nov. with strain SG 3301 T as the type strain (=DSM 15116 T ).

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Halobacteroidaceae

Oren

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Orenia salinaria sp. nov., a fermentative bacterium isolated from anaerobic sediments of Mediterranean salterns.

Cited by 41 publications

References 30 publications

Orenia metallireducens sp. nov. Strain Z6, a Novel Metal-Reducing Member of the Phylum Firmicutes from the Deep Subsurface

Orenia metallireducens sp. nov. Strain Z6, a Novel Metal-Reducing Member of the Phylum Firmicutes from the Deep Subsurface

Isolation and characterization of spirilloid purple phototrophic bacteria forming red layers in microbial mats of Mediterranean salterns: description of Halorhodospira neutriphila sp. nov. and emendation of the genus Halorhodospira

Halobacteroidaceae

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