Orexin A but not orexin B regulates lipid metabolism and leptin secretion in isolated porcine adipocytes

Pruszyńska‐Oszmałek, Ewa; Kołodziejski, Paweł; Kaczmarek, P.; Sassek, Maciej; Szczepankiewicz, Dawid; Mikuła, Robert; Nowak, Krzysztof W.

doi:10.1016/j.domaniend.2017.12.003

Cited by 20 publications

(17 citation statements)

References 31 publications

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“…Furthermore, orexin A suppresses glycerol release as well as Hsl mRNA expression in 3T3-L1 adipocytes (Skrzypski et al 2011), which is consistent with findings reported in humans (Digby et al 2006). Suppression of lipolysis by orexin A but not orexin B was also reported in isolated porcine adipocytes (Pruszynska-Oszmalek et al 2018). Overall, these in vitro results suggest that orexin A exerts its lipogenic and antilipolytic activities by acting directly on adipocytes.…”

Section: The Role Of Orexins In Regulating Mature White Adipocytes Fusupporting

confidence: 62%

“…Furthermore, both orexin receptors are present in porcine subcutaneous and visceral adipose tissue as well as in mature porcine visceral adipocytes on mRNA and protein levels. Noteworthy, the expression of OXR1 is higher compared to OXR2 in both investigated fat depots and isolated adipocytes (Pruszynska-Oszmalek et al 2018).…”

Section: Expression Of Orexin and Orexin Receptors In White Adipose Tmentioning

confidence: 82%

“…In vivo, orexin A can reduce leptin levels in rats and mice (Switonska et al 2002, Park et al 2015. By contrast orexin A stimulates leptin secretion and expression in porcineisolated adipocytes (Pruszynska-Oszmalek et al 2018).…”

Section: The Role Of Orexins In Regulating Mature White Adipocytes Fumentioning

confidence: 97%

“…This process requires activation of PI3-K/PKB pathway that in turn leads to enhanced translocation of the glucose transporter GLUT4 to the plasma membrane (Skrzypski et al 2011). Orexin A enhances glucose uptake by stimulating GLUT4 expression in porcine adipocytes (Pruszynska-Oszmalek et al 2018). GLUT4 plays a prominent role in insulin-induced glucose uptake and its selective depletion in adipose tissue causes insulin resistance (Abel et al 2001).…”

Section: The Role Of Orexins In Regulating Mature White Adipocytes Fumentioning

confidence: 99%

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The role of orexin in controlling the activity of the adipo-pancreatic axis

Skrzypski

Billert

Nowak

et al. 2018

Journal of Endocrinology

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Orexin A and B are two neuropeptides, which regulate a variety of physiological functions by interacting with central nervous system and peripheral tissues. Biological effects of orexins are mediated through two G-protein-coupled receptors (OXR1 and OXR2). In addition to their strong influence on the sleep-wake cycle, there is growing evidence that orexins regulate body weight, glucose homeostasis and insulin sensitivity. Furthermore, orexins promote energy expenditure and protect against obesity by interacting with brown adipocytes. Fat tissue and the endocrine pancreas play pivotal roles in maintaining energy homeostasis. Since both organs are crucially important in the context of pathophysiology of obesity and diabetes, we summarize the current knowledge regarding the role of orexins and their receptors in controlling adipocytes as well as the endocrine pancreatic functions. Particularly, we discuss studies evaluating the effects of orexins in controlling brown and white adipocytes as well as pancreatic alpha and beta cell functions.

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Section: The Role Of Orexins In Regulating Mature White Adipocytes Fusupporting

confidence: 62%

Section: Expression Of Orexin and Orexin Receptors In White Adipose Tmentioning

confidence: 82%

Section: The Role Of Orexins In Regulating Mature White Adipocytes Fumentioning

confidence: 97%

Section: The Role Of Orexins In Regulating Mature White Adipocytes Fumentioning

confidence: 99%

See 2 more Smart Citations

The role of orexin in controlling the activity of the adipo-pancreatic axis

Skrzypski

Billert

Nowak

et al. 2018

Journal of Endocrinology

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“…A more recent study showed that orexin A, but not orexin B, induced significant uptake of glucose by isolated rat adipocytes. In addition, it was also shown that orexin A stimulated mRNA expression of glucose transporter 4 in fat cells.…”

Section: Hypocretins/orexins and Diabetes Mellitusmentioning

confidence: 94%

Hypocretin/orexin modulates body weight and the metabolism of glucose and insulin

Adeghate

Lotfy

D’Souza

et al. 2020

Diabetes Metabolism Res

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Summary The hypocretin/orexin (Hcrt/orexin) unit affects the functions of the nervous, cardiovascular, gastrointestinal, and reproductive systems. Hcrt/orexin ligands and receptors have been localized to different parts of the central and peripheral nervous systems, cerebrospinal fluid and blood, exocrine (pancreas, salivary, lacrimal) as well as endocrine (pancreatic islets, pituitary, adrenal) glands. Several factors including stress, glucagon‐like peptide‐1 agonists, glutamate, nicotine, glucose, and hypoglycaemia stimulate the expression of Hcrt/orexin system, but it is inhibited by ageing, bone morphogenetic protein, hypoxia/hypercapnia, melanocortin receptor accessory protein 2, and glucagon. Literature reports show that Hcrt/orexin can significantly increase insulin secretion from normal and diabetic rat pancreata. Hcrt/orexin decreases blood glucose concentration and reduces insulin resistance partly via increased tissue expression of glucose transporter type 4. It reduces obesity by increasing browning of fat cells and energy expenditure. Taken together, Hcrt/orexin modulates obesity and the metabolism of glucose and insulin. The Hcrt/orexin system may thus be a target in the development of new therapies for the treatment of diabetes mellitus.

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orexin B alleviates sepsis‐associated lung injury through the attenuation of pulmonary endothelial barrier dysfunction by regulating the rho‐associated coiled‐coil containing protein kinase 2/zonula occludens‐1 (ROCK2/ZO‐1) axis

Wang,

Wan,

2024

Biotech and App Biochem

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Dysfunction of the alveolar endothelial barrier plays a crucial role in the pathogenesis of septic acute lung injury (ALI). orexin B is a neuropeptide derived from orexin neurons in the lateral hypothalamus and has multiple biological functions. However, the physiological function of orexin B in sepsis is less reported. Here, we aimed to explore the protective effects of orexin B in sepsis‐induced ALI and its underlying mechanisms. In this study, we established an ALI in vivo animal model in mice using cecal ligation and puncture (CLP) and an in vitro ALI model using mouse lung microvascular endothelial cells (MLMECs) induced with lipopolysaccharides (LPS). The animal experiments involved four groups: Sham, Sham+orexin B, CLP, CLP+orexin B. First, our results demonstrate that the levels of serum orexin B but not orexin A were reduced in septic mice. Correspondingly, the expression of orexin type 2 receptor (OX2R), but not orexin type 1 receptor (OX1R), was reduced in the lung tissue of septic mice. Administration of orexin B decreased the mortality in sepsis mice and improved M‐CASS scores. Hematoxylin–eosin (H&E) staining assay demonstrated that administration of orexin B ameliorated histopathological lung injury. orexin B was also found to inhibit the inflammatory response in the lung tissues of septic mice by reducing the expression of tumor necrosis factor α (TNF‐α), interleukin 6 (IL‐6), and recombinant chemokine C‐X‐C‐motif ligand 15 (CXCL15). Additionally, the total cell count and neutrophils in bronchoalveolar lavage fluid (BALF) were reduced by orexin B. Notably, orexin B alleviated vascular endothelial permeability in mice lung tissue by increasing the expression of the tight junction protein zonula occludens‐1 (ZO‐1) and occludin. In vitro experiments demonstrated that orexin B prevented LPS‐induced endothelial permeability in mouse lung microvascular endothelial cells (MLMECs) by upregulating the expression of ZO‐1 and occludin. These effects are mediated by rho‐associated coiled‐coil containing protein kinase 2 (ROCK2). Based on these findings, we conclude that orexin B alleviates sepsis‐induced ALI by ameliorating endothelial permeability of lung microvascular endothelial cells.

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Orexin A but not orexin B regulates lipid metabolism and leptin secretion in isolated porcine adipocytes

Cited by 20 publications

References 31 publications

The role of orexin in controlling the activity of the adipo-pancreatic axis

The role of orexin in controlling the activity of the adipo-pancreatic axis

Hypocretin/orexin modulates body weight and the metabolism of glucose and insulin

orexin B alleviates sepsis‐associated lung injury through the attenuation of pulmonary endothelial barrier dysfunction by regulating the rho‐associated coiled‐coil containing protein kinase 2/zonula occludens‐1 (ROCK2/ZO‐1) axis

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