Orexinergic actions modify occurrence of slow inward currents on neurons in the pedunculopontine nucleus

Kovács, Attila; Baksa, Brigitta; Bayasgalan, Tsogbadrakh; Szentesi, Péter; Csemer, Andrea; Pál, Balázs

doi:10.1097/wnr.0000000000001298

Cited by 1 publication

(1 citation statement)

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“…The strain was purchased from Jackson Laboratories (Bar Harbor, ME, USA). The reason for employing this strain was to use mice without any differences in phenotype compared to the wild-type mice, but to have the same genetical background of the strains used in our previous studies on SICs [28,35,49]. Experiments on mice were in accordance with the appropriate international (EU Directive 2010/63/EU for animal experiments), national and institutional guidelines and law on the care of research animals by the Ethical Committee for Animal Research, University of Debrecen, Hungary (approval number: 19/2019/DEMÁB).…”

Section: Animals Preparationmentioning

confidence: 99%

Pharmacological Activation of Piezo1 Channels Enhances Astrocyte–Neuron Communication via NMDA Receptors in the Murine Neocortex

Csemer,

Sokvári,

Maamrah

et al. 2024

IJMS

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The Piezo1 mechanosensitive ion channel is abundant on several elements of the central nervous system including astrocytes. It has been already demonstrated that activation of these channels is able to elicit calcium waves on astrocytes, which contributes to the release of gliotransmitters. Astrocyte- and N-methyl-D-aspartate (NMDA) receptor-dependent slow inward currents (SICs) are hallmarks of astrocyte–neuron communication. These currents are triggered by glutamate released as gliotransmitter, which in turn activates neuronal NMDA receptors responsible for this inward current having slower kinetics than any synaptic events. In this project, we aimed to investigate whether Piezo1 activation and inhibition is able to alter spontaneous SIC activity of murine neocortical pyramidal neurons. When the Piezo1 opener Yoda1 was applied, the SIC frequency and the charge transfer by these events in a minute time was significantly increased. These changes were prevented by treating the preparations with the NMDA receptor inhibitor D-AP5. Furthermore, Yoda1 did not alter the spontaneous EPSC frequency and amplitude when SICs were absent. The Piezo1 inhibitor Dooku1 effectively reverted the actions of Yoda1 and decreased the rise time of SICs when applied alone. In conclusion, activation of Piezo1 channels is able to alter astrocyte–neuron communication. Via enhancement of SIC activity, astrocytic Piezo1 channels have the capacity to determine neuronal excitability.

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Section: Animals Preparationmentioning

confidence: 99%