Organ culture of mucosal biopsies of human small intestine

Browning, Thomas H.; Trier, Jerry S.

doi:10.1172/jci106108

Cited by 344 publications

(138 citation statements)

References 20 publications

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“…Organ culture ofintestinal explants is a well-established technique for maintaining both adult (Browning & Trier, 1969) and foetal (Simon-Assmann et al, 1982) tissue for periods up to 48 h. We have previously adapted organ culture for pig small-intestinal explants and have shown that the morphology, at both the light-and the electron-microscopic level, is well preserved during culture up to 24 h (Danielsen et al, 1982a). The explants also maintained their contents of protein and microvillar enzymes and were able to sustain a continuous protein synthesis during the entire culture period.…”

Section: Resultsmentioning

confidence: 99%

Translational control of an intestinal microvillar enzyme

Danielsen¹,

Cowell²,

Sjöström³

et al. 1986

Biochemical Journal

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The rates of biosynthesis of adult and foetal pig small-intestinal aminopeptidase N (EC 3.4.11.2) were compared to determine at which level the expression of the microvillar enzyme is developmentally controlled. In organ-cultured explants, the rate of biosynthesis of foetal aminopeptidase N is only about 3% of the adult rate. The small amount synthesized occurs in a high-mannose-glycosylated, membrane-bound, form that is processed to the mature, complex-glycosylated, form at a markedly slower rate than that of the adult enzyme. Extracts of total RNA from adult and foetal intestine contained comparable amounts of aminopeptidase N mRNA, encoding gel-electrophoretically identical primary translation products. Together, these data indicate that the expression of aminopeptidase N is controlled at a translational level.

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Section: Resultsmentioning

confidence: 99%

Translational control of an intestinal microvillar enzyme

Danielsen¹,

Cowell²,

Sjöström³

et al. 1986

Biochemical Journal

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“…Explants were cultured as described by Browning and Trier [29] with modifieations according to Naim et al [30] and Lottaz et al [31]. In brief, after 2 h in methionine-free medium explants were labelled continuously for 6 h with 150 ~tCi [~S]methionine (Amersham) in 1 ml medium.…”

Section: Organ Culture and Tmmwtoisolationmentioning

confidence: 99%

The levels of lactase and of sucrase‐isomaltase along the rabbit small intestine are regulated both at the mRNA level and post‐translationally

et al. 1992

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We determined along the small intestine of young and adult rabbits the activities of lactase (LPH) and sucrase (Sl), the levels of their cognate mRNA~, and examined the in vitro biosynthesis of LPH and pro.Sl. Lactate activity is low in the proximal 1/3 of the intestine, whereas the mRNA levels are high. However, the rates of biosynthesis of the LPH forma correlated well with the steady-state levels of LPH mRNA in all see~nents, indicating that factor(s) acting post-translationally produce a decline in brush border LPH in the proximal small intestine. These factor(s) are not involved in the processing of pro-LPH to mat ure LPH, since the relative amounts ol'the various l'onns of LPH are almost the same :dong the small intestine. Unexpectedly, we find that also for SI the ratio of activity to mRNA is low in proximal intestine. The biosynthesis ol'pro-Sl ~rrclates with the steady-state levels of its mRNA. Hence, the steady-state levels o1" LPI-I and Sl along the small intestine arc regulated both by mRNA level5 attd by posttranslational factor(s).

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“…A ring of filter paper surrounding the central well was saturated with 150 mmol I -' NaCl and the culture dishes were placed in an incubator with humidified air, at 37 C . The organ culture technique followed that described and validated by Browning & Trier [14]. Control samples, boiled for 10 min in 150 mmol I -' NaCl were handled in a similar way.…”

Section: Methodsmentioning

confidence: 99%

Arachidonic acid absorption in human jejunum in organ culture: effects of ethanol

Barros

CHEN

Florén

et al. 1990

Eur J Clin Investigation

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Abstract. Human jejunal tissue obtained by peroral biopsy was cultured in control medium and in medium containing 100 mmol I-' ethanol. Subsequently, the incorporation and metabolism of [3H]-arachidonic and [14C]-linoleic acid were evaluated. Of the two fatty acids a significantly higher amount of ['HI-arachidonic acid was incorporated into phospholipids and more [14C]-linoleic acid was incorporated into triacylglycer01s. This preferential distribution of the labelled fatty acids was not affected by ethanol, but when ethanol was present in the tissue culture medium, there was a significant decrease in the incorporation of both fatty acids into tissue phospholipids. The study thus shows that ethanol in moderate concentrations can affect human jejunal absorption and metabolism of polyenoic fatty acids, contributing to a decrease in the amount of eicosanoid precursors available in jejunal tissue.

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Organ culture of mucosal biopsies of human small intestine

Cited by 344 publications

References 20 publications

Translational control of an intestinal microvillar enzyme

Translational control of an intestinal microvillar enzyme

The levels of lactase and of sucrase‐isomaltase along the rabbit small intestine are regulated both at the mRNA level and post‐translationally

Arachidonic acid absorption in human jejunum in organ culture: effects of ethanol

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