Organ-Derived Decellularized Extracellular Matrix: A Game Changer for Bioink Manufacturing?

Choudhury, Deepak; Tun, Han W.; Wang, Tian-Yi; Naing, May Win

doi:10.1016/j.tibtech.2018.03.003

Cited by 235 publications

(221 citation statements)

References 79 publications

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“…The 72 h pepsin digestion required for generating human lung dECM hydrogels was substantially longer than that described for decellularized tissue ECMs from other organs (10). In concert with our findings, Pouliot and colleagues recently described decellularization and gelation of porcine lung using a pepsin digestion of 42 h (22).…”

Section: L702supporting

confidence: 79%

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Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue

Hilster

Sharma²,

Jonker

et al. 2020

American Journal of Physiology-Lung Cellular and Molecular Phys

120

115

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de Hilster RHJ, Sharma PK, Jonker MR, White ES, Gercama EA, Roobeek M, Timens W, Harmsen MC, Hylkema MN, Burgess JK. Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue. Chronic lung diseases such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD) are associated with changes in extracellular matrix (ECM) composition and abundance affecting the mechanical properties of the lung. This study aimed to generate ECM hydrogels from control, severe COPD [Global Initiative for Chronic Obstructive Lung Disease (GOLD) IV], and fibrotic human lung tissue and evaluate whether their stiffness and viscoelastic properties were reflective of native tissue. For hydrogel generation, control, COPD GOLD IV, and fibrotic human lung tissues were decellularized, lyophilized, ground into powder, porcine pepsin solubilized, buffered with PBS, and gelled at 37°C. Rheological properties from tissues and hydrogels were assessed with a low-load compression tester measuring the stiffness and viscoelastic properties in terms of a generalized Maxwell model representing phases of viscoelastic relaxation. The ECM hydrogels had a greater stress relaxation than tissues. ECM hydrogels required three Maxwell elements with slightly faster relaxation times () than that of native tissue, which required four elements. The relative importance (R i) of the first Maxwell element contributed the most in ECM hydrogels, whereas for tissue the contribution was spread over all four elements. IPF tissue had a longer-lasting fourth element with a higher R i than the other tissues, and IPF ECM hydrogels did require a fourth Maxwell element, in contrast to all other ECM hydrogels. This study shows that hydrogels composed of native human lung ECM can be generated. Stiffness of ECM hydrogels resembled that of whole tissue, while viscoelasticity differed.

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Section: L702supporting

confidence: 79%

“…Upon pH neutralization and bringing to physiological osmolarity, hydrogels form spontaneously at 37°C. As such, these hydrogels comprise the native ECM composition, albeit not the macroscopic (micrometer sized) architecture (10,23).…”

Section: Introductionmentioning

confidence: 99%

Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue

Hilster

Sharma²,

Jonker

et al. 2020

American Journal of Physiology-Lung Cellular and Molecular Phys

120

115

View full text Add to dashboard Cite

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“…It has been shown that high voltages can alter the structure of proteins (Freedman, Haq, Edel, et al, 2013). Therefore, while both collagen denaturing and protein dissolution are possible during processing, the retained bioactivity from the ECM within the scaffold outweighs the losses seen (Badylak, Freytes, & Gilbert, 2009;Choudhury, Tun, Wang, & Naing, 2018).…”

Section: Discussionmentioning

confidence: 99%

Hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering

Reid

Callanan

2019

J Biomed Mater Res

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The aim when designing a scaffold is to provide a supportive microenvironment for the native cells, which is generally achieved by structurally and biochemically imitating the native tissue. Decellularized extracellular matrix (ECM) possesses the mechanical and biochemical cues designed to promote native cell survival. However, when decellularized and reprocessed, the ECM loses its cell supporting mechanical integrity and architecture. Herein, we propose dissolving the ECM into a polymer/solvent solution and electrospinning it into a fibrous sheet, thus harnessing the biochemical cues from the ECM and the mechanical integrity of the polymer. Bovine aorta and myocardium were selected as ECM sources. Decellularization was achieved using sodium dodecyl sulfate (SDS), and the ECM was combined with polycaprolactone and hexafluoro‐2‐propanol for electrospinning. The scaffolds were seeded with human umbilical vein endothelial cells (HUVECs). The study found that the inclusion of aorta ECM increased the scaffold's wettability and subsequently lead to increased HUVEC adherence and proliferation. Interestingly, the inclusion of myocardium ECM had no effect on wettability or cell viability. Furthermore, gene expression and mechanical changes were noted with the addition of ECM. The results from this study show the vast potential of electrospun ECM/polymer bioscaffolds and their use in tissue engineering.

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“…Engineering the composition of bioinks with bioactive compounds to guide cell response often makes use of cues derived from the native extracellular environment, eventually creating an artificial, and mostly chemically defined, yet simplified ECM substitute. Rather than designing “bottom‐up” a bioink composition, a powerful approach is to use natural templates, as already provided by native ECM . Printable, cell compatible bioinks have been obtained by decellularizing and solubilizing ECM from adipose tissue, cartilage and cardiac muscle, and each of these bioinks was proven superior to collagen gels in view of their potential to induce differentiation of adipose derived stromal cells, bone‐marrow derived stromal cells, and cardiomyocites, respectively .…”

Section: Strategies To Evolve From Shape To Functionmentioning

confidence: 99%

From Shape to Function: The Next Step in Bioprinting

et al. 2020

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In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell‐laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.

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Organ-Derived Decellularized Extracellular Matrix: A Game Changer for Bioink Manufacturing?

Cited by 235 publications

References 79 publications

Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue

Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue

Hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering

From Shape to Function: The Next Step in Bioprinting

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