Organ donation and transplantation: Awareness and roles of healthcare professionals—A systematic literature review

Jawoniyi, Oluwafunmilayo; Gormley, Kevin; McGleenan, Emma; Noble, Helen

doi:10.1111/jocn.14154

Cited by 78 publications

(56 citation statements)

References 51 publications

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“…The key to successful donor management in the CC setting is significantly associated with the ICU and CC nurses' attitudes toward OD, and by that will have an impact on the availability of organs for individuals who need life-saving organ transplant treatment (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). Before 2011 no studies measuring ATODA existed in the worldwide clinical context.…”

Section: Discussionmentioning

confidence: 99%

Psychometric properties of the North American version of the Flodén ATODAI (Attitudes Toward Organ Donor Advocacy Instrument): a validation study

Flodén¹,

Stadtler

Collazo

et al. 2020

Preprint

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Background: Intensive and critical-care nurses are the key to successful donor management in the critical-care setting. No studies measuring attitudes toward organ donor advocacy existed before 2011, when the 51-item Swedish “Attitudes Toward Organ Donor Advocacy Scale” was developed. The aim of this study was to translate, adapt and establish the psychometric properties of the North American version of the Flodén ATODAI (Attitudes Toward Organ Donor Advocacy Instrument) in terms of validity and reliability. Methods: A multi-step approach was used: Initial translation; Back-translation; Review and synthesis of these translations; Expert panel (N=7) rated the prefinal version of the instrument for content validity index (CVI); International panel made adjustments guided by the expert panel . Reliability testing with test and retest of the adjusted 46-item version was conducted using intraclass correlation coefficient (ICC), weighted kappa ( ҡ Weight ), sign test, and Cronbach’s alpha coefficient (α), (N=50); and finally Delphi technique procedure with a preselected Delphi panel (N=15). Results: The CVI was determined to be greater than the 0.05 significance level. Item level (I-CVI) ranged 0.82-1.0, with a mean of 0.97. Scale level (S-CVI) on the entire instrument was 0.97. Test-retest procedure was performed to estimate stability. In total, 34 of the items had good-to-high ICC. Accepting an ICC of > 0.70 resulted in a total of 24 items. Homogeneity reliability was estimated by α and was calculated for these items where α=0.90. In total, 20 of the items had a substantial or almost perfect ҡ Weight and 23 showed a moderate ҡ Weight . None of the items showed systematical differences. The Delphi technique procedure was used on the 22 items with ICC <0.70 resulted in adjustments establishing that consensus was achieved. Conclusions: Undertaking this multi-step, cross-cultural adaptation procedure has effectively ensured that the 46-item Flodén ATODAI [North America version] produces valid and reliable measurements.

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Section: Discussionmentioning

confidence: 99%

Psychometric properties of the North American version of the Flodén ATODAI (Attitudes Toward Organ Donor Advocacy Instrument): a validation study

Flodén¹,

Stadtler

Collazo

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…role has an impact upon the organ donation and by that the organ transplantation process. The care by specialist nurses is the key to successful donor management in the critical-care (CC) setting since their actions and behavior are signi cantly associated with authorization to, or decline of, organ donation (OD) (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). In addition, ICU nurses' attitudes have an impact on the availability of organs for individuals who need life-saving organ transplant treatment (5,(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Cross-cultural adaptation and psychometric validation of the Flodén ATODAI instrument in the North American context

Flodén¹,

Stadtler

Collazo

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Intensive and critical-care nurses are the key to successful donor management in the critical-care setting. No studies measuring attitudes toward organ donor advocacy existed before 2011, when the 51-item Swedish “Attitudes Toward Organ Donor Advocacy Scale” was developed. The aim of this study was to translate, adapt and establish the psychometric properties of the North American version of the Flodén ATODAI (Attitudes Toward Organ Donor Advocacy Instrument) in terms of validity and reliability. Methods: A multi-step approach was used: Initial translation; Back-translation; Review and synthesis of these translations; Expert panel (N=7) rated the prefinal version of the instrument for content validity index (CVI); International panel made adjustments guided by the expert panel. Reliability testing with test and retest of the adjusted 46-item version was conducted using intraclass correlation coefficient (ICC), weighted kappa (ҡ Weight), sign test, and Cronbach’s alpha coefficient (α), (N=50); and finally Delphi technique procedure with a preselected Delphi panel (N=15).Results: The CVI was determined to be greater than the 0.05 significance level. Item level (I-CVI) ranged 0.82-1.0, with a mean of 0.97. Scale level (S-CVI) on the entire instrument was 0.97. Test-retest procedure was performed to estimate stability. In total, 34 of the items had good-to-high ICC. Accepting an ICC of >0.70 resulted in a total of 24 items. Homogeneity reliability was estimated by α and was calculated for these items where α=0.90. In total, 20 of the items had a substantial or almost perfect ҡ Weight and 23 showed a moderate ҡ Weight. None of the items showed systematical differences. The Delphi technique procedure was used on the 22 items with ICC <0.70 resulted in adjustments establishing that consensus was achieved.Conclusions: Undertaking this multi-step, cross-cultural adaptation procedure has effectively ensured that the 46-item Flodén ATODAI [North American version] produces valid and reliable measurements.

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“…The shortage of human organs for allotransplantation has become a severe problem; therefore, scientists worldwide are dedicating much effort to developing limitless replacement sources for organs, for example, by producing human stem cell‐derived chimeric organs or gene‐edited pig organs . Despite the fact that great progress has already been achieved recently, long‐term porcine liver graft survival could still be problematic compared to heart or kidney xenotransplantation.…”

Section: Introductionmentioning

confidence: 99%

Immortalization of porcine hepatocytes with a α‐1,3‐galactosyltransferase knockout background

Wang

Zhang

Wang

et al. 2019

Xenotransplantation

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Background In vivo pig liver xenotransplantation preclinical trials appear to have poor efficiency compared to heart or kidney xenotransplantation because of xenogeneic rejection, including coagulopathy, and particularly thrombocytopenia. In contrast, ex vivo pig liver (wild type) perfusion systems have been proven to be effective in “bridging” liver failure patients until subsequent liver allotransplantation, and transgenic (human CD55/CD59) modifications have even prolonged the duration of pig liver perfusion. Despite the fact that hepatocyte cell lines have also been proposed for extracorporeal blood circulation in conditions of acute liver failure, porcine hepatocyte cell lines, and the GalT‐KO background in particular, have not been developed and applied in this field. Herein, we established immortalized wild‐type and GalT‐KO porcine hepatocyte cell lines, which can be used for artificial liver support systems, cell transplantation, and even in vitro studies of xenotransplantation. Methods Primary hepatocytes extracted from GalT‐KO and wild‐type pigs were transfected with SV40 LT lentivirus to establish immortalized GalT‐KO porcine hepatocytes (GalT‐KO‐hep) and wild‐type porcine hepatocytes (WT). Hepatocyte biomarkers and function‐related genes were assessed by immunofluorescence, periodic acid‐Schiff staining, indocyanine green (ICG) uptake, biochemical analysis, ELISA, and RT‐PCR. Furthermore, the tumorigenicity of immortalized cells was detected. In addition, a complement‐dependent cytotoxicity (CDC) assay was performed with GalT‐KO‐hep and WT cells. Cell death and viability rates were assessed by flow cytometry and CCK‐8 assay. Results GalT‐KO and wild‐type porcine hepatocytes were successfully immortalized and maintained the characteristics of primary porcine hepatocytes, including albumin secretion, ICG uptake, urea and glycogen production, and expression of hepatocyte marker proteins and specific metabolic enzymes. GalT‐KO‐hep and WT cells were confirmed as having no tumorigenicity. In addition, GalT‐KO‐hep cells showed less apoptosis and more viability than WT cells when exposed to complement and xenogeneic serum. Conclusions Two types of immortalized cell lines of porcine hepatocytes with GalT‐KO and wild‐type backgrounds were successfully established. GalT‐KO‐hep cells exhibited higher viability and injury resistance against a xenogeneic immune response.

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Organ donation and transplantation: Awareness and roles of healthcare professionals—A systematic literature review

Abstract: There is a suggestion that healthcare professionals partly account for the global shortage in organ donation, but there is a need to examine how healthcare professionals' roles, knowledge, awareness, skills and competencies might impact upon the organ donation and transplantation process.

Cited by 78 publications

References 51 publications

Psychometric properties of the North American version of the Flodén ATODAI (Attitudes Toward Organ Donor Advocacy Instrument): a validation study

Psychometric properties of the North American version of the Flodén ATODAI (Attitudes Toward Organ Donor Advocacy Instrument): a validation study

Cross-cultural adaptation and psychometric validation of the Flodén ATODAI instrument in the North American context

Immortalization of porcine hepatocytes with a α‐1,3‐galactosyltransferase knockout background

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