Organ-on-a-Chip

Yoon, Jeong‐Yeol

doi:10.1007/978-3-030-83696-2_11

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…[130][131][132][133] The applications of microfluidics range from lab-on-chip platforms to organ-on-chip devices. [134][135][136][137][138][139][140][141][142][143][144][145][146][147][148] Since DNA-or RNA-based diagnostic requires large numbers of nucleic acid to perform amplification, amplification of the original count of nucleic acid is essential for testing. In general, there are two types of nucleic acid amplification techniques: 1) temperature cycling and 2) isothermal amplification.…”

Section: Loc and Microfluidic-based Lampmentioning

confidence: 99%

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

et al. 2022

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The loop‐mediated isothermal amplification (LAMP) method is one of the Nucleic acid amplification tests (NAATs) that allows for the amplification of target regions without using a thermal cycle. With its unique primer design, LAMP ensures the rapid replication of the targeted DNA region with high specificity and high efficiency. LAMP technology is used for diagnostic purposes in pathogen detection due to its ease of use, low cost, and simplicity without requiring complex equipment. A wide range of LAMP diagnostic platforms have been developed for applications in bacteria, virus, and parasitic pathogen detection. Herein, the methodology of LAMP technology and its applications in pathogen detection and SNP genotyping and mutation detection are discussed. Point‐of‐care (PoC) LAMP platforms designed with the principles of microfluidic chip technology, including LAMP‐on‐a‐chip, paper‐based LAMP, and smartphone‐based LAMP applications have been elaborated. LAMP technology represents a fast, robust, and reliable diagnostic platform for point‐of‐care testing.

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Section: Loc and Microfluidic-based Lampmentioning

confidence: 99%

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

et al. 2022

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“…[28] OOC systems are convenient, versatile means of mimicking the functions of various organs of the human body with the ability to be seeded with human cells to create patient-specific, multicellular setups for conducting personalized medicine research and an environment for studying realistic organ interactions with proposed therapeutic approaches. [29][30][31][32][33][34] The main advantages offered by microchannels, chambers, valves, and pumps, for cell culture, may include perfusability and possible gas permeability (which increase cell viability and metabolic rate), transparency (which enables microscopic imaging), [35,36] integrability with sensors (which allows real-time screening of culture, biomarkers, and responses to stimuli), [37,38] gradient generation as a result of laminar flow in microchannels (which enables the study of differentiation and directed cell migration), porous membranes (modeling tissue barrier functions, transcellular transport, secretion, and absorption), cost-efficiency (lower volume of expensive samples/reagents due to microscale channels), sophisticated structures (wide range of manufacturable geometries on microfluidic chips), mimicking of dynamic in vivo conditions (emulating cyclic mechanical stress and strain experienced by cells during peristalsis, respiration, and cardiovascular cycling), and/or single-cell analysis. [5,10,29] Conventional OOC fabrication approaches (e.g., soft lithography, microcontact printing, and replica molding [39,40] ) usually require cleanrooms, a high level of microfabrication expertise, [41,42] a secondary cellseeding step (resulting in intense protein absorption), and have problems implementing cell-cell and cell-ECM interactions to emulate spatial heterogeneity.…”

Section: Introductionmentioning

confidence: 99%

3D bioprinted organ‐on‐chips

et al. 2022

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Organ-on-a-chip (OOC) platforms recapitulate human in vivo-like conditions more realistically compared to many animal models and conventional two-dimensional cell cultures. OOC setups benefit from continuous perfusion of cell cultures through microfluidic channels, which promotes cell viability and activities. Moreover, microfluidic chips allow the integration of biosensors for real-time monitoring and analysis of cell interactions and responses to administered drugs. Three-dimensional (3D) bioprinting enables the fabrication of multicell OOC platforms with sophisticated 3D structures that more closely mimic human tissues. 3D-bioprinted OOC platforms are promising tools for understanding the functions of organs, disruptive influences of diseases on organ functionality, and screening the efficacy as well as toxicity of drugs on organs. Here, common 3D bioprinting techniques, advantages, and limitations of each method are reviewed. Additionally, recent advances, applications, and potentials of 3D-bioprinted OOC platforms for emulating various human organs are presented. Last, current challenges and future perspectives of OOC platforms are discussed. K E Y W O R D S biomaterials, bioprinting, disease-on-a-chip, microfluidics, organ-on-a-chip 1 This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Customizable Microfluidic Devices: Progress, Constraints, and Future Advances

Aljabali,

Obeid,

Mishra

et al. 2024

CDD

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The field of microfluidics encompasses the study of fluid behavior within micro-channels and the development of miniature systems featuring internal compartments or passageways tailored for fluid control and manipulation. Microfluidic devices capitalize on the unique chemical and physical properties exhibited by fluids at the microscopic scale. In contrast to their larger counterparts, microfluidic systems offer a multitude of advantages. Their implementation facilitates the investigation and utilization of reduced sample, solvent, and reagent volumes, thus yielding decreased operational expenses. Owing to their compact dimensions, these devices allow for the concurrent execution of multiple procedures, leading to expedited experimental timelines. Over the past two decades, microfluidics has undergone remarkable advancements, evolving into a multifaceted discipline. Subfields such as organ-on-a-chip and paper-based microfluidics have matured into distinct fields of study. Nonetheless, while scientific progress within the microfluidics realm has been notable, its translation into autonomous end-user applications remains a frontier to be fully explored. This paper sets forth the central objective of scrutinizing the present research paradigm, prevailing limitations, and potential prospects of customizable microfluidic devices. Our inquiry revolves around the latest strides achieved, prevailing constraints, and conceivable trajectories for adaptable microfluidic technologies. We meticulously delineate existing iterations of microfluidic systems, elucidate their operational principles, deliberate upon encountered limitations, and provide a visionary outlook toward the future trajectory of microfluidic advancements. In summation, this work endeavors to shed light on the current state of microfluidic systems, underscore their operative intricacies, address incumbent challenges, and unveil promising pathways that chart the course toward the next frontier of microfluidic innovation.

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Organ-on-a-Chip

Cited by 3 publications

References 13 publications

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

3D bioprinted organ‐on‐chips

Customizable Microfluidic Devices: Progress, Constraints, and Future Advances

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