Organ perfusion techniques in drug development

Stretch, Graham L.; Nation, Roger L.; Evans, Allan M.; Milne, Robert

doi:10.1002/(sici)1098-2299(199903/04)46:3/4<292::aid-ddr15>3.0.co;2-4

Cited by 11 publications

(9 citation statements)

References 24 publications

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“…22 Therefore, this methodological gap between testing new cancer therapies in humans, on the one hand, and animal models, on the other hand, can be avoided using ex-vivo perfusion techniques of surgically resected organs under cold storage. 23 Considering these data and the important role of albumin in tumor metabolism, we have used human albumin bound to multiwalled CNTs (MWCNTs) for the selective targeting of PC cells. Since ethical limitations made the selectivity and therapeutic potential of these nanocompounds in patients impossible to test, we have designed an original model of living PC.…”

Section: Introductionmentioning

confidence: 99%

Selective ex-vivo photothermal ablation of human pancreatic cancer with albumin functionalized multiwalled carbon nanotubes

Mocan

Tabaran²,

Mocan³

et al. 2011

IJN

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The process of laser-mediated ablation of cancer cells marked with biofunctionalized carbon nanotubes is frequently called “nanophotothermolysis”. We herein present a method of selective nanophotothermolisys of pancreatic cancer (PC) using multiwalled carbon nanotubes (MWCNTs) functionalized with human serum albumin (HSA). With the purpose of testing the therapeutic value of these nanobioconjugates, we have developed an ex-vivo experimental platform. Surgically resected specimens from patients with PC were preserved in a cold medium and kept alive via intra-arterial perfusion. Additionally, the HSA-MWCNTs have been intra-arterially administered in the greater pancreatic artery under ultrasound guidance. Confocal and transmission electron microscopy combined with immunohistochemical staining have confirmed the selective accumulation of HSA-MWCNTs inside the human PC tissue. The external laser irradiation of the specimen has significantly produced extensive necrosis of the malign tissue after the intra-arterial administration of HSA-MWCNTs, without any harmful effects on the surrounding healthy parenchyma. We have obtained a selective photothermal ablation of the malign tissue based on the selective internalization of MWCNTs with HSA cargo inside the pancreatic adenocarcinoma after the ex-vivo intra-arterial perfusion.

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Section: Introductionmentioning

confidence: 99%

Selective ex-vivo photothermal ablation of human pancreatic cancer with albumin functionalized multiwalled carbon nanotubes

Mocan

Tabaran²,

Mocan³

et al. 2011

IJN

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“…The liver perfusion apparatus used in our laboratory is based on the description of Miller et al but whit changes [9][10][11]. The perfusion apparatus consisted of a perfused reservoir maintained at 37 °C, that perfusion was carried out by a peristaltic pump at a constant flow rate of 20 ml/min for 120 min.…”

Section: Perfusion Apparatusmentioning

confidence: 99%

“…Central vein pressure (C.V.P) linear was used for measurement H2O pressure and serum set was used for attachment elements of apparatus and degassing perfusate. The selected mode for liver perfusion is singlepass because AFB 1 is high hepatic extraction [9].…”

Section: Perfusion Apparatusmentioning

confidence: 99%

Co-Exposure Effects of LPS with Various Aflatoxin B1 Doses in Isolated Perfused Rat Liver Model

Koohi

Staji

Hayati

et al. 2017

IJT

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“…In most publication about liver perfusion experiments modified Krebs‐Henseleit‐buffer (KHB) is applied as the experimental perfusion liquid, e.g 8–11. It contains plenty of glucose (2000 mg/L) and salts (e.g.…”

Section: Introductionmentioning

confidence: 99%

Development and application of a simplified sample preparation method for determination of TEGDMA and related metabolites

et al. 2009

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Analysis of biological samples obtained from in vivo experiments can be often challenging. In general it is not possible to apply the commonly used matrices that are necessary for the experiments to the desired analysis systems without further conditioning or sample purification steps. Besides possible adverse effects for instruments, interference between analytes and matrices can affect the correct measurement of analytes. Different methods of sample preparation can be used to convert biological samples into samples suitable for analysis; SPE and HS-SPME are two well established methods. Research of in vivo metabolism of triethyleneglycoledimethacrylate (TEGDMA), one of the most frequently contained comonomer in dental restorative materials, demands sample preparation methods that offer separation of TEGDMA and its related metabolites from biological matrices. In the presented study two methods for sample preparation were developed in order to analyze TEGDMA as well as its metabolites triethyleneglycole (TEG), 2,3-epoxymethacrylicacid methylester (2,3-EMME), and methacrylacid methylester (MAME) in Krebs-Henseleit buffer samples to facilitate a subsequent analysis via GC-MS. An easy and time-saving separation protocol was developed. Recovery rates of TEGDMA and TEG after SPE were 21 +/- 3% and 105 +/- 12%, respectively, recovery rate after headspace extraction of 2,3-EMME and MAME was higher at 48 degrees C compared with 20 degrees C extraction temperature. The tested range for 2,3-EMME and MAME concentration after HS-SPME extraction was 0.1-100 mg/L and both analytes showed a good linearity.

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Organ perfusion techniques in drug development

Cited by 11 publications

References 24 publications

Selective ex-vivo photothermal ablation of human pancreatic cancer with albumin functionalized multiwalled carbon nanotubes

Selective ex-vivo photothermal ablation of human pancreatic cancer with albumin functionalized multiwalled carbon nanotubes

Co-Exposure Effects of LPS with Various Aflatoxin B1 Doses in Isolated Perfused Rat Liver Model

Development and application of a simplified sample preparation method for determination of TEGDMA and related metabolites

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