Organ specific changes in energy metabolism due to anaerobiosis in the sea mussel Mytilus edulis (L.)

Zurburg, W.; Kluytmans, J.H.

doi:10.1016/0305-0491(80)90150-9

Cited by 28 publications

(20 citation statements)

References 9 publications

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“…Volatile fatty acids were assayed gaschromatographically according to Kluytmans et al (1975). Free amino acids were analyzed as described by Zurburg and Kluytmans (1980). For isotachophoretic analysis the perchloric acid extract was neutralized with K2CO 3 and percolated through Dowex 1 × 2 (OH).…”

Section: Determination Of Metabolitesmentioning

confidence: 99%

Aspects of anaerobic metabolism in Anodonta cygnea L.

Holwerda

Veenhof

1984

Comparative Biochemistry and Physiology Part B: Comparative Bio

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Abstract--1. After 6 days of anoxia A. cygnea had produced 1.0 pmole succinate, 2.7 pmole propionate and 1.7/~mole acetate/g of total soft tissue (wet). In addition, 0.35 #mole glutamate had disappeared. No other changes were detectable.2. Concentrations of anaerobic end products and of amino acids differed from organ to organ. Large portions of all end products produced were present in the fluids within the animal.3. Acetate and propionate were also excreted into the surrounding water. 4. In all tissues examined the activity ratio of pyruvate kinase and phosphoenolpyruvate carboxykinase was high 5. The [PEP]0. 5 of pyruvate kinase was relatively low (0.04-0.05 mM at pH 7.5), and the enzyme is not strongly inhibited at decreased pH or in the presence of alanine.6. Anaerobiosis influences this enzyme by decreasing the activity at low substrate concentration.

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Section: Determination Of Metabolitesmentioning

confidence: 99%

Aspects of anaerobic metabolism in Anodonta cygnea L.

Holwerda

Veenhof

1984

Comparative Biochemistry and Physiology Part B: Comparative Bio

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“…The shell valves were slightly pried open with an oyster knife, allowing water to drain off the mantle cavity. Approximately 0.5 m1 hemolymph was obtained by directly puncturing the sinus of the posterior adductor muscle using a syringe (0.45 X 12 mm, Terumo Europe, Leuven, Bclyium) (Zurburg & Kluytmans 1980). The shell valves were opened by cutting the posterior adductor muscle.…”

Section: Methodsmentioning

confidence: 99%

Bioavailability of dissolved zinc to the common mussel Mytilus edulis in complexing environments

Vercauteren¹,

Blust²

1996

Mar. Ecol. Prog. Ser.

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The bioavailability of d~ssolved zinc to the common mussel Mjftllus edulis was studied in chemically defined seawater containing 5 different organic ligands. Zinc uptake shows saturable kinetics, indicating that it is a facilitated process. The uptake of zinc from solution is nearly linear over a 24 h period for mussel tissues exposed to a 5 pm01 I-' concentration. In most cases, metal complexation decreases zinc uptake by reducing the activity of the free metal ion. Zinc uptake rates are similar for 4 of the 5 ligands studied when the free zinc ion activ~ty is kept constant. Complexation of zinc with histidine facilitates the uptake of zlnc. Whether the n~echanism of facilitation involves the direct uptake of zinc-histidlne complexes across the membrane cannot be deduced from the present study. For the same free zinc ion activity, the avallabillty of zinc is higher in the digestive system than in the gills. The observed differences can be caused by changes in the chemical speciation of the metals in the layers lining the exchange surfaces where conditions may deviate considerably from seawater.

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“…The onset of anaerobic metabolism is indicated by the accumulation of succinate, a key anaerobic metabolite of bivalve mitochondria (Zurburg and Kluytmans, 1980;Sukhotin and Pörtner, 1999;Hines et al, 2007). In fact, a sharp increase in succinate concentration occurred in mantle tissue above 28°C (Fig.…”

Section: Thermal Limits Under Normocapniamentioning

confidence: 99%

Impact of ocean acidification on thermal tolerance and acid–base regulation of Mytilus edulis (L.) from the North Sea

Zittier

Bock

Lannig

et al. 2015

Journal of Experimental Marine Biology and Ecology

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Anthropogenic climate change confronts marine organisms with rapid trends of concomitant warming and CO 2 induced ocean acidification. The survival and distribution of species partly depend on their ability to exploit their physiological plasticity during acclimatization. Therefore, in laboratory studies the effects of simulated future ocean acidification on thermal tolerance, energy metabolism and acid-base regulation capacity of the North Sea population of the blue mussel Mytilus edulis were examined. Following one month of pre-acclimation to 10°C and control CO 2 levels, mussels were exposed for two weeks to control and projected oceanic CO 2 levels (390, 750 and 1120 μatm) before being subjected to a stepwise warming protocol between 10°C and 31°C (+3°C each night). Oxygen consumption and heart rates, anaerobic metabolite levels and haemolymph acidbase status were determined at each temperature. CO 2 exposure left oxygen consumption rate unchanged at acclimation temperature but caused a somewhat stronger increase during acute warming and thus mildly higher Q 10 -values than seen in controls. Interestingly, the thermally induced limitation of oxygen consumption rate set in earlier in normocapnic than in hypercapnic (1120 μatm CO 2 ) mussels (25.2°C vs. 28.8°C), likely due to an onset of metabolic depression in the control group following warming. However, the temperature induced increase in heart rate became limited above 25°C in both groups indicating an unchanged pejus temperature regardless of CO 2 treatment. An upper critical temperature was reached above 28°C in both treatments indicated by the accumulation of anaerobic metabolites in the mantle tissue, paralleled by a strong increase in haemolymph PCO 2 at 31°C. Ocean acidification caused a decrease in haemolymph pH. The extracellular acidosis remained largely uncompensated despite some bicarbonate accumulation. In all treatments animals developed a progressive warming-induced extracellular acidosis. A stronger pH drop at around 25°C was followed by stagnating heart rates. However, normocapnic mussels enhanced bicarbonate accumulation at the critical limit, a strategy no longer available to hypercapnic mussels. In conclusion, CO 2 has small effects on the response patterns of mussels to warming, leaving thermal thresholds largely unaffected. High resilience of adult North Sea mussels to future ocean acidification indicates that sensitivity to thermal stress is more relevant in shaping the response to future climate change.

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Organ specific changes in energy metabolism due to anaerobiosis in the sea mussel Mytilus edulis (L.)

Cited by 28 publications

References 9 publications

Aspects of anaerobic metabolism in Anodonta cygnea L.

Aspects of anaerobic metabolism in Anodonta cygnea L.

Bioavailability of dissolved zinc to the common mussel Mytilus edulis in complexing environments

Impact of ocean acidification on thermal tolerance and acid–base regulation of Mytilus edulis (L.) from the North Sea

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