Organ-Specific Regulation of the CD8 T Cell Response toListeria monocytogenesInfection

Abstract

The intestinal mucosal CD8 T cell response to infection with Listeria monocytogenes was measured using MHC class I tetramers and was compared with the response in peripheral blood, secondary lymphoid tissue, and liver. To assess the vaccination potential of Listeria and to analyze responses in C57BL/6 mouse strains, a recombinant Listeria expressing OVA (rLM-ova) was generated. The response peaked at 9 days postinfection with a much larger fraction of the intestinal mucosa and liver CD8 T cell pool OVA specific, as compared with the spleen. However, these differences were not linked to bacterial titers in each site. The higher responses in lamina propria and liver resulted in a larger CD8 memory population in these tissues. Furthermore, the level of memory induced was dependent on infectious dose and inversely correlated with the magnitude of the recall response after oral challenge. Recall responses in the tissues were most robust in the lamina propria and liver, and reactivated Ag-specific T cells produced IFN-γ. Infection of CD40- or MHC class II-deficient mice induced poor CD8 T cell responses in the intestinal mucosa, but only partially reduced responses in the spleen and liver. Overall, the results point to novel pathways of tissue-specific regulation of primary and memory antimicrobial CD8 T cell responses.

Persistent Antigen Presentation after Acute Vesicular Stomatitis Virus Infection

“…A dose of 5 ϫ 10 4 PFU of VSV-Ova or VSV-Ind suspended in 50 l Hanks' balanced saline solution (HBSS) was delivered intranasally using a 200-l pipette. In some experiments, 1 ϫ 10 3 CFU of Listeria monocytogenes encoding ovalbumin (40) was injected via the tail vein, while 1 ϫ 10 4 CFU was used as the intranasal dose. For antibiotic treatment to clear L. monocytogenes infection, ampicillin (2 mg/ml) was administered in the drinking water for 7 days.…”

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

“…Mice immunized with 10.000 CFU rLM-OVA at day 6 were used as positive control and unimmunized mice served as negative control. rLM-OVA [27,28] were cultured in Brain Hart Infusion broth (BHI; Sigma-Aldrich) with 5 μg/ ml erythromycin and to challenge the mice 100.000 bacteria from a LOG-phase culture were injected in 200 μl/mouse in the tail vain. To study the elimination of bacteria, three days after challenge spleens were isolated and single cell suspensions were made in RPMI medium.…”

“…Journal of Controlled Release 266 (2017) [27][28][29][30][31][32][33][34][35] 3.5. Protective immune response towards recombinant rLM-OVA after intradermal immunization using hollow microneedles CD8 + T cells play an essential role in clearance of the intracellular bacterium Listeria monocytogenes [31].…”

Differences in maintenance of CD8+ and CD4+ bacteria‐specific effector‐memory T cell populations

“…Intraepithelial lymphocytes (IEL), lamina propria-derived lymphocytes (LPL), and Peyer's patch-derived lymphocytes (PP) were isolated as described previously [37]. Briefly, the entire small intestine was removed and washed, and the Peyer's patches were cut off.…”