Organelle-Redirected Chameleon Sensor-Enabled Live Cell Imaging of Mitochondrial DNA

Abstract

Mitochondrial DNA (mtDNA) plays important roles in diverse physiological processes and myriad diseases. We herein report mtDNA imaging with a chameleon sensor containing a cationic rhodamine B (RB) entity for mitochondria targeting and a fluorogenic SYBR Green-I (SG) entity for DNA sensing. SG-RB selectively binds to mtDNA and gives green SG fluorescence in mitochondria of living cells but gives red RB fluorescence upon delivery of mitochondria into lysosomes in mitophagy. With the dual-color imaging, mtDNA aggregation and elevated mitophagy were identified in HeLa cells stressed with anticancer doxorubicin. These results suggest the utility of organelle-redirected DNA sensors for live cell imaging of mtDNA involved in myriad pathological disorders.

Synthesis of blue fluorescent carbon dots and their application in detecting mercury and iodine based on “off–on” mode

“…The concentration of CD we used in cell culture was 0.04 mg·mL −1 , and this concentration is greater than that reported in much of the literature for HeLa cell cultures, meaning it is a relatively large concentration. [ 52–55 ] Although HeLa cells were immersed in the CD solution for 48 h, cells were able to move freely and stayed alive without outside nourishment. More than 95% of the cells were alive, which proved that the synthesized material was not toxic to the cells (Figure 11f).…”

An Activatable and Switchable Nanoaggregate Probe for Detecting H2S and Its Application in Mice Brains

“…[33][34][35][36] Among these diseases, the mtDNA level is remarkably affected in specific positions such as brain and heart, while these positions are also the key ones for H 2 Sassociated diagnose. [37][38][39] Although a variety of small molecular fluorescent probes have been reported for detecting H 2 S or mtDNA independently, [40][41][42][43] in this work, we attempted to combine them into a detecting system which was activatable, switchable and available for applications in brains.…”

Nucleic Acids Analysis

“…Han and his coworkers reported an organelle-trappable DNA sensor (SG-RB) for dual-color imaging of mitochondrial DNA (mtDNA). As shown in Figure 6(b), SG-RB selectively bound mtDNA in mitochondria, thereby giving rise to turnon green fluorescence [196]. Tang and his coworkers presented a multicolor nanoflares based novel nanoprobe that can detect and image three kinds of tumor-related mRNAs in living cells simultaneously.…”

“…Various nanostructurebased SERS tags were developed to improve the detection signals for sensitively and precisely determining miRNAs [195]. (b) "Turn-on" fluorescence imaging of mtDNA with SG-RB [196]. (c) Intracellular imaging of c-myc mRNA, TK1 mRNA, and GalNAc-T mRNA under CLSM [197].…”