Organic Acid and Solvent Production: Butanol, Acetone, and Isopropanol; 1,3- and 1,2-Propanediol Production; and 2,3-Butanediol Production

Chen, Jiann-Shin; Zidwick, Mary Jo; Rogers, Palmer

doi:10.1007/978-3-642-31331-8_386

Cited by 7 publications

(6 citation statements)

References 316 publications

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“…With the growing concern of petroleum shortage and the negative environmental impact of fossil resource extraction and transformation, there is a renewed worldwide interest for environmentally friendly ways to produce fuels and chemicals (Chen et al 2013 ). Alcohols are natural major end products of some microbial fermentations.…”

Section: Introductionmentioning

confidence: 99%

Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling

Gérando

Fayolle-Guichard

Rudant

et al. 2016

Appl Microbiol Biotechnol

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Random mutagenesis and genome shuffling was applied to improve solvent tolerance and isopropanol/butanol/ethanol (IBE) production in the strictly anaerobic bacteria Clostridium beijerinckii DSM 6423. Following chemical mutagenesis with N-methyl-N-nitro-N-nitrosoguanidine (NTG), screening of putatively improved strains was done by submitting the mutants to toxic levels of inhibitory chemicals or by screening for their tolerance to isopropanol (>35 g/L). Suicide substrates, such as ethyl or methyl bromobutyrate or alcohol dehydrogenase inhibitors like allyl alcohol, were tested and, finally, 36 mutants were isolated. The fermentation profiles of these NTG mutant strains were characterized, and the best performing mutants were used for consecutive rounds of genome shuffling. Screening of strains with further enhancement in isopropanol tolerance at each recursive shuffling step was then used to spot additionally improved strains. Three highly tolerant strains were finally isolated and able to withstand up to 50 g/L isopropanol on plates. Even if increased tolerance to the desired end product was not always accompanied by higher production capabilities, some shuffled strains showed increased solvent titers compared to the parental strains and the original C. beijerinckii DSM 6423. This study confirms the efficiency of genome shuffling to generate improved strains toward a desired phenotype such as alcohol tolerance. This tool also offers the possibility of obtaining improved strains of Clostridium species for which targeted genetic engineering approaches have not been described yet.

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Section: Introductionmentioning

confidence: 99%

Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling

Gérando

Fayolle-Guichard

Rudant

et al. 2016

Appl Microbiol Biotechnol

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“…This route utilizes the pyruvate generated during glycolysis to render acetoin and 2,3‐butanediol as accumulated products . The 2,3‐butanediol is less toxic to microorganisms than other alcohols such as ethanol and butanol, making it possible to obtain higher yields …”

Section: Introductionmentioning

confidence: 99%

“…Biotechnological production of 2,3‐butanediol dates back to last century but is being reexamined due to its environmental benefits. The main advantage of producing this chemical by biological synthesis is to reduce the use of fossil materials, especially when using renewable substrates . In this sense, agave bagasse can be used as raw material for 2,3‐butanediol synthesis, as it is an abundant lignocellulosic residue derived from production of the Mexican alcoholic beverages known as Mezcal and Tequila.…”

Section: Introductionmentioning

confidence: 99%

“…The 2,3-butanediol is less toxic to microorganisms than other alcohols such as ethanol and butanol, making it possible to obtain higher yields. 3,12,13 Biotechnological production of 2,3-butanediol dates back to last century but is being reexamined due to its environmental benefits. The main advantage of producing this chemical by biological synthesis is to reduce the use of fossil materials, especially when using renewable substrates.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, K. oxytoca is a facultative anaerobic microorganism that can metabolize pentoses and hexoses, which is particularly useful because hydrolysates obtained from lignocellulosic biomass generally are rich in pentoses. 2,4,5,7,12 Klebsiella oxytoca has been used to produce 2,3-butanediol from molasses, 11,27 corn cob hydrolysates, 28,29 Jatropha hulls hydrolysates, 30 wood hydrolysates, 31 sorghum stalk hydrolysates, 13,32 switchgrass hydrolysates, 32 whey and cassava wastewater, 33 and simultaneously utilizing glucose and galactose obtained from the green algae Golenkinia sp. hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

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Production of 2,3‐butanediol by fermentation of enzymatic hydrolysed bagasse from agave mezcal‐waste using the native Klebsiella oxytoca UM2‐17 strain

Pasaye-Anaya

Vargas‐Tah

Martínez‐Cámara

et al. 2019

J of Chemical Tech & Biotech

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Background 2,3‐butanediol is a chemical component with wide industrial applications, such as biofuels and chemical precursors. Reducing 2,3‐butanediol production costs is a high priority and recent focus has been on its biological synthesis from raw renewable materials rather than through conventional chemical procedures. In this study, a 2,3‐butanediol native bacterial producer was selected for the fermentation of Agave cupreata bagasse waste by enzymatic hydrolysation to obtain 2,3‐butanediol. Results The native Klebsiella oxytoca UM2‐17 strain was selected to efficiently produce 2,3‐butanediol on synthetic mineral media. Using glucose (100 g L−1) and glucose: xylose (50 g L−1:50 g L−1) as sugar sources obtained titres of 29.36 g L−1 and 25.9 g L−1 of 2,3‐butanediol, corresponding to yields of 0.29 g g−1 and 0.26 g g−1, respectively. Agave bagasse solid waste after acid pre‐treatment was utilized to obtain enzymatic hydrolysates, obtaining liquors with glucose/xylose/arabinose at 25.8 g L−1 with efficiency of ∼52.5% sugar conversion from residual solid pre‐hydrolysed bagasse. The production of 2,3‐butanediol through batch fermentation of the enzymatic‐hydrolysate by the UM2‐17 strain showed total depletion of glucose and xylose, achieving a maximal production of 10.3 g L−1 of 2,3‐butanediol plus 0.28 g L−1 ethanol, corresponding to 2,3‐butanediol yield of 0.40 g g−1. Conclusion The results indicate that A. cupreata bagasse waste from mezcal beverage elaboration is a sustainable bioresource for production of 2,3‐butanediol by fermentation of enzymatic bagasse‐hydrolysate using the K. oxytoca UM2‐17 strain. © 2019 Society of Chemical Industry

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Biobutanol production from apple pomace: the importance of pretreatment methods on the fermentability of lignocellulosic agro-food wastes

Hijosa‐Valsero

Paniagua-García

Díez-Antolínez

2017

Appl Microbiol Biotechnol

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Apple pomace was studied as a possible raw material for biobutanol production. Five different soft physicochemical pretreatments (autohydrolysis, acids, alkalis, organic solvents and surfactants) were compared in a high-pressure reactor, whose working parameters (temperature, time and reagent concentration) were optimised to maximise the amount of simple sugars released and to minimise inhibitor generation. The pretreated biomass was subsequently subjected to a conventional enzymatic treatment to complete the hydrolysis. A thermal analysis (DSC) of the solid biomass indicated that lignin was mainly degraded during the enzymatic treatment. The hydrolysate obtained with the surfactant polyethylene glycol 6000 (PEG 6000) (1.96% w/w) contained less inhibitors than any other pretreatment, yet providing 42 g/L sugars at relatively mild conditions (100 °C, 5 min), and was readily fermented by Clostridium beijerinckii CECT 508 in 96 h (3.55 g/L acetone, 9.11 g/L butanol, 0.26 g/L ethanol; 0.276 g/g yield; 91% sugar consumption). Therefore, it is possible to optimise pretreatment conditions of lignocellulosic apple pomace to reduce inhibitor concentrations in the final hydrolysate and perform successful ABE fermentations without the need of a detoxification stage.

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Organic Acid and Solvent Production: Butanol, Acetone, and Isopropanol; 1,3- and 1,2-Propanediol Production; and 2,3-Butanediol Production

Cited by 7 publications

References 316 publications

Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling

Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling

Production of 2,3‐butanediol by fermentation of enzymatic hydrolysed bagasse from agave mezcal‐waste using the native Klebsiella oxytoca UM2‐17 strain

Biobutanol production from apple pomace: the importance of pretreatment methods on the fermentability of lignocellulosic agro-food wastes

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