Organic acid pretreatment of oil palm trunk: effect on enzymatic saccharification and ethanol production

Rattanaporn, Kittipong; Tantayotai, Prapakorn; Phusantisampan, Theerawut; Pornwongthong, Peerapong; Sriariyanun, Malinee

doi:10.1007/s00449-017-1881-0

Cited by 70 publications

(20 citation statements)

References 34 publications

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“…3). The efficiency of cellulase enzyme was compared based on the hydrolysis product in the form of reducing sugar [22][23]. Testing with CMC substrate, the reducing sugars obtained from a total 32 samples were ranged from 0.51-4.35 mg/mL (Fig.…”

Section: Composition Of Cassava Pulpmentioning

confidence: 99%

Analysis of Microbial Consortia with High Cellulolytic Activities for Cassava Pulp Degradation

et al. 2020

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Biogas production is one of the means to manage the cassava pulp waste obtained from the cassava processing plants. The success of the process is determined by the hydrolysis in an anaerobic digester. When the digester failure is found, the new microbial consortium inoculum is introduced to the system with the long period of set up time. This research aimed to construct the endemic microbial consortium by re-cultivating the cellulolytic microbial consortia obtained from cassava pulp and digester wastewater with the expected shorter set up time. Modifications of enrichment and re-cultivation methods by varying the nutrients, pH and temperature improved the enzymatic hydrolysis yields, as reducing sugars, of CMC, rice straw and cassava pulp substrates approximately 9, 3, and 13 times, respectively. To analyze the enzymatic activities of the selected microbial consortia, the cellulase enzyme was extracted, partially purified and analyzed on CMC-zymogram. The ~130 kDa-sized cellulase enzyme was identified with endocellulase activity, and it was considered as a relatively large molecular size molecule compared to most bacterial endocellulases. The selected microbial consortia were tested for their biomass degradation capacities, and the optimal operational condition was obtained at pH 7.0 and 30 °C. This optimal condition showed the proof of the concept that this re-cultivated consortium could be applied in on-site digester with high efficiency.

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Section: Composition Of Cassava Pulpmentioning

confidence: 99%

Analysis of Microbial Consortia with High Cellulolytic Activities for Cassava Pulp Degradation

et al. 2020

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“…After 24 hours of fermentation, glucose and xylose could be almost completely metabolized and the ethanol concentration was 12.59 g/L, corresponding to an ethanol yield of 73.6%. It has been reported that oil palm trunk treated with different organic acid were enzymatically hydrolyzed and used as substrate for ethanol fermentation, citric acid was the most efficient organic acid for pretreatment to enhance ethanol production compared to acetic acid and oxalic acid [25].…”

Section: Enzymatic Hydrolysis and Ethanol Productionmentioning

confidence: 99%

“…These studies showed that dicarboxylic organic acids can hydrolyze β-1,4 bonds more selectively than sulfuric acid [24]. Except to these dicarboxylic acids, citric acid was also used to pretreatment of oil palm trunk [25]. It also demonstrated a similar good performance of hemicellulose and lignin removal from oil palm trunk.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Dilute Organic Acid Pretreatment and a Comprehensive Exploration of Citric Acid Pretreatment on Corn Cob

Qiao¹,

Cui²,

Ouyang³

et al. 2019

Journal of Renewable Materials

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As an important agricultural waste, corn cob is one of the most abundant lignocellulosic biomass. In this work, dilute acid pretreatment using seven different organic acids were compared for compositional analysis of corn cob. The first pKa of organic acids, influenced by the number and type of the side group and distance between two carboxylic groups, played a crucial role in the pretreatment effect. Citric acid was found to have better pretreatment performance than other tested dicarboxylic acids. Under the condition of 30 mM, 160°C and 60 min, the level of xylose reached 22.64 g/L in pretreatment liquid, and the pretreated corn cob demonstrated the high enhancement of enzymatic hydrolysis up to 93.4% and the ethanol yield was 73.6%.

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“…There were reports that OPF biomass was able to enhance enzymatic hydrolysis with the proper pre-treatment approach [4]. Other parts of the oil palm waste which were utilized for biorefinery includes empty fruit bunch and oil palm trunk [5,6]. OPF was discovered to be able to produce renewable sugars simply by pressing the fresh OPF to obtain the juice or sap [7].…”

Section: Introductionmentioning

confidence: 99%

Application of Michaelis–Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production

et al. 2020

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Ferulic acid (FA) production has become a frequent focus on today's research due to its antioxidant properties. However, there has been little to none studies reported on the usage of mixed culture as inoculum in FA production. This study aims to determine the kinetics FA production in a mixed culture fermentation by applying the Michaelis-Menten kinetic model. In this study, mixed culture fermentation process using oil palm frond bagasse (OPFB) as substrate was applied for FA production improvement. Optimum condition was applied to study ferulic acid esterase (FAE) mechanism for kinetic modelling purposes. The kinetic model used was based on the Michaelis-Menten kinetic model. Runge-Kutta Fourth Order method was used to solve the kinetic model. Maximum FAE activity was achieved at the 28 h of fermentation process at 3.7 × 10 −3 mU mL −1. This result proved that enzymatic hydrolysis occurred during fermentation process. Kinetic study was conducted with Michaelis-Menten kinetic model used as a reference kinetic equation. Three kinetic constants, V max , K m and K s were determined as 3.725 × 10 −3 g L −1 h −1 , 28.231 g L −1 and 1.33 × 10 −2 h −1 respectively using Runge-Kutta Fourth Order approach. The outcome of this study confirms that the kinetics of the process fit well with the Michaelis-Menten model. This also suggests that the usage of mixed culture has the potential to induce enzymatic hydrolysis hence improving FA production from OPFB during fermentation process.

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Organic acid pretreatment of oil palm trunk: effect on enzymatic saccharification and ethanol production

Cited by 70 publications

References 34 publications

Analysis of Microbial Consortia with High Cellulolytic Activities for Cassava Pulp Degradation

Analysis of Microbial Consortia with High Cellulolytic Activities for Cassava Pulp Degradation

Comparison of Dilute Organic Acid Pretreatment and a Comprehensive Exploration of Citric Acid Pretreatment on Corn Cob

Application of Michaelis–Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production

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