Organic carbon quantity and quality jointly triggered the switch between dissimilatory nitrate reduction to ammonium (DNRA) and denitrification in biofilters

Sun, Feng; Deng, Qinghui; Li, Xiaowen; Tang, Mengjuan; Ma, Xiaoqi; Cao, Xiuyun; Zhou, Yiyong; Song, Changchun

doi:10.1016/j.chemosphere.2021.130917

Cited by 19 publications

(3 citation statements)

References 37 publications

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“…Candidatus Nitrotoga is a low-temperature-tolerant nitrifying bacterium ( Skoyles et al, 2020 ); its presence in the WH-DR might therefore have contributed to removal of NH 4 + -N throughout the experimental period. In a corn cob-supported denitrification system, Anaeromyxobacter was mainly involved in dissimilatory nitrate reduction to ammonium (DNRA) ( Sun et al, 2021 ); therefore, DNRA may also have occurred in the present study. Spirochaeta , a bacterium with hemicellulose-degrading ability, was previously present in a wastewater denitrification system using corn cob as filler ( Zhao et al, 2015 ), and its main contribution to this study was the degradation of WH.…”

Section: Resultsmentioning

confidence: 78%

Effects of hydraulic retention time and influent nitrate concentration on solid-phase denitrification system using wheat husk as carbon source

Niu

Gao

Zhang

et al. 2023

PeerJ

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Solid-phase denitrification shows promise for removing nitrate (NO3−-N) from water. Biological denitrification uses external carbon sources to remove nitrogen from wastewater, among which agriculture waste is considered the most promising source due to its economic and efficiency advantages. Hydraulic retention time (HRT) and influent nitrate concentration (INC) are the main factors influencing biological denitrification. This study explored the effects of HRT and INC on solid-phase denitrification using wheat husk (WH) as a carbon source. A solid-phase denitrification system with WH carbon source was constructed to explore denitrification performance with differing HRT and INC. The optimal HRT and INC of the wheat husk-denitrification reactor (WH-DR) were 32 h and 50 mg/L, respectively. Under these conditions, NO3−-N and total nitrogen removal rates were 97.37 ± 2.68% and 94.08 ± 4.01%, respectively. High-throughput sequencing revealed that the dominant phyla in the WH-DR operation were Proteobacteria, Bacteroidetes, and Campilobacterota. Among the dominant genera, Diaphorobacter (0.85%), Ideonella (0.38%), Thiobacillus (4.22%), and Sulfurifustis (0.60%) have denitrification functions; Spirochaeta (0.47%) is mainly involved in the degradation of WH; and Acidovorax (0.37%) and Azospira (0.86%) can both denitrify and degrade WH. This study determined the optimal HRT and INC for WH-DR and provides a reference for the development and application of WH as a novel, slow-release carbon source in treating aquaculture wastewater.

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Section: Resultsmentioning

confidence: 78%

Effects of hydraulic retention time and influent nitrate concentration on solid-phase denitrification system using wheat husk as carbon source

Niu

Gao

Zhang

et al. 2023

PeerJ

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“…Whereas NH + 4 -N was found with a low inlet concentration of 1.85 mg/L and 0.45 mg/L, respectively, and a relatively higher outlet concentration during the above reaction process. The inlet wastewater with excess carbon source (TOC) was speculated to combine with different species and facilitate the switch of dissimilatory nitrate reduction to ammonium (DNRA) over denitrification during the initial stage of the entire reactor operation [38]. The conversion of nitrate to ammonium might be beneficial to the denitrification process; microorganisms were capable of using a relatively low level of ammonium in the system.…”

Section: Substance Conversion Paths and Mechanisms Analysismentioning

confidence: 99%

Nitrate Removal from Actual Wastewater by Coupling Sulfur-Based Autotrophic and Heterotrophic Denitrification under Different Influent Concentrations

Liu

Wang

Zhang

et al. 2021

Water

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Contamination of wastewater with organic-limited nitrates has become an urgent problem in wastewater treatment. The cooperating heterotrophic with sulfur autotrophic denitrification is an alternative process and the efficiency has been assessed in many studies treating simulated wastewater under different operating conditions. However, due to the complex and diverse nature of actual wastewater, more studies treating actual wastewater are still needed to evaluate the feasibility of collaborative denitrification. In this study, lab-scale experiments were performed with actual nitrate polluted water of two different concentrations, with glucose and sodium thiosulfate introduced as mixed electron donors in the coupling sulfur-based autotrophic and heterotrophic denitrification. Results showed that the optimum denitrification performance was exhibited when the influent substrate mass ratio of C/N/S was 1.3/1/1.9, with a maximum denitrification rate of 3.52 kg NO3−-N/(m3 day) and nitrate removal efficiency of 93% in the coupled systems. Illumina high-throughput sequencing analysis revealed that autotrophic, facultative, and heterotrophic bacteria jointly contributed to high nitrogen removal efficiency. The autotrophic denitrification maintained as the predominant process, while the second most prevalent denitrification process gradually changed from heterotrophic to facultative with the increase of influent concentration at optimum C/N/S ratio conditions. Furthermore, the initiation of dissimilatory nitrate reduction to ammonium (DNRA) was very pivotal in promoting the entire denitrification process. These results suggested that sulfur-based autotrophic coupled with heterotrophic denitrifying process is an alternative and promising method to treat nitrate containing wastewater.

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“…As an alternative to simple organics, a variety of cheap biopolymer materials which contain cellulose, hemicellulose, or lignin (e.g., starch, cotton, corncob, wheat straw, rice husk, sawdust, and woodchip) have been developed as alternative electron donors for solid-phase denitrification [10][11][12][13][14][15]. Additionally, corncob is considered a better biopolymer electron donor with a higher N removal rate than straw, rice husks, rice straw, wheat straw, corn stalk, soybean stalk, and soybean hull in different studies [16,17].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Microbial and Metabolic Mechanisms in an Aerobic Bioreactor with Immobilized Microflora by Simple and Complex Electron Donors

Deng

Wang²,

Wang

et al. 2023

Water

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Microflora immobilization is promising for nutrient removal applications in sewage; however, the metabolic and microbial mechanism needs to be further explored. Heterotrophic nitrification-aerobic denitrification (HN-AD) bacterium and efficient nitrogen (N) removal bacteria were selected and immobilized on corncob particles using alginate polymer to prepare microbe–organic complex beads. The complex beads were then added into activated sludge under a continuous-flow aerobic bioreactor with sufficient sodium acetate also applied as a simple electron donor. The role of polymer electron donors under carbon-rich conditions was then studied. Results showed that the total nitrogen removal rate improved by 8.3% (reaching 91.2%) and ammonium nitrogen removal rates were approximately 98%. Only 0.59 mg/L of nitrate nitrogen was detected in the treatment group. 16S rRNA gene sequencing results showed that bacterial richness in activated sludge within the treatment group was significantly higher than within the control group (p < 0.05), and KEGG pathways analysis indicated that carbon (C) metabolism gene and N-cycle-related genes were also improved. This suggested that polymer electron donors generated complex C sources that nourished diverse bacterial species related to N cycles so that the N removal rate could be strengthened and further improved by simple electron donors and the microflora.

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Organic carbon quantity and quality jointly triggered the switch between dissimilatory nitrate reduction to ammonium (DNRA) and denitrification in biofilters

Cited by 19 publications

References 37 publications

Effects of hydraulic retention time and influent nitrate concentration on solid-phase denitrification system using wheat husk as carbon source

Effects of hydraulic retention time and influent nitrate concentration on solid-phase denitrification system using wheat husk as carbon source

Nitrate Removal from Actual Wastewater by Coupling Sulfur-Based Autotrophic and Heterotrophic Denitrification under Different Influent Concentrations

Enhancement of Microbial and Metabolic Mechanisms in an Aerobic Bioreactor with Immobilized Microflora by Simple and Complex Electron Donors

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