Microorganisms play
vital roles in the biogeochemical processes,
such as biomethane production, which is an important source of coal
bed methane (CBM), that occur in coal beds. However, little information
is available regarding the microbial communities in in situ coal beds
of different ranks from Permian mining areas. In this study, anaerobic
Biolog ECO microplates were used to determine the microbial metabolic
activity, and high-throughput sequencing was used to investigate the
bacteria and archaea communities in three typical mining areas. Anaerobic
Biolog ECO microplates showed that the peak value of average well
color development (AWCD) in low-rank coal seams from Anhui Province
(A-L) was 0.46 higher than that of medium-rank coal from Shanxi Province
(S-M). Phylogenetic analysis indicated that the bacterial phyla Proteobacteria,
Firmicutes, and Actinobacteria were the predominant lineages in all
samples, in particular, the family Oxalobacteraceae accounted for
the overwhelming majority of the bacterial sequences (from 47.20 to
92.67%). Most of the identified genera, Herbaspirillum, Pseudomonas, and Acinetobacter, were fermentative bacteria that can convert coal macromolecules
into methanol, acetic acid, and CO2. In addition, the archaea
in A-L were dominated by the phyla Euryarchaeota, Thaumarchaeota,
and Woesearchaeota. Additionally, Euryarchaeota was the dominant archaeal
lineage in S-M, and Thaumarchaeota was predominant in the Guizhou
Province group of high-rank coals (G-H). Canonical correspondence
analysis (CCA) showed that there were significant correlations between
the microbial community and porosity, hydrogen content, and oxygen
content (p < 0.05). The KEGG pathway analysis
indicated that biomethane production mainly depended on archaea and
that the methane metabolism capacity of A-L coals was the highest.
Overall, the results suggest that the diversities of the microbial
communities were related to the porosity and chemical elements of
the different ranks of coal.