Organic-inorganic Interfaces for a New Generation of Hybrid Biosensors

Rea, Ilaria; Giardina, Paola; Longobardi, Sara; Giocondo, M.; Stefano, Luca De

doi:10.5772/17057

Cited by 3 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phase imaging tool also allows recognizing regions with different elasticity (Figure 2) [24,32] or other structural heterogeneities, such as crystalline/amorphous composition of polymers, by recording offsets and phase angles of input signal variations respect to those of the oscillating cantilever. In particular, the visualization of amorphous and crystalline components in polymers is extremely interesting, since it is able to highlight edges and it provides fine observation of peculiar structures, which are often obscured by a rough topography investigation [33].…”

Section: Working Principles and Basic Toolsmentioning

confidence: 99%

“…AFM has been traditionally evaluated as a powerful technique to perform an accurate measurement of surface roughness as well as to investigate failure mechanisms of polymer scaffolds [32,33,34,35]. The understanding surface properties at sub-angstrom size scale are becoming tremendously relevant to explore the main features of scaffold at nanoscale.…”

Section: Working Principles and Basic Toolsmentioning

confidence: 99%

See 1 more Smart Citation

Atomic Force Microscopy: A Powerful Tool to Address Scaffold Design in Tissue Engineering

Marrese

Guarino

Ambrosio

2017

JFB

108

View full text Add to dashboard Cite

Functional polymers currently represent a basic component of a large range of biological and biomedical applications including molecular release, tissue engineering, bio-sensing and medical imaging. Advancements in these fields are driven by the use of a wide set of biodegradable polymers with controlled physical and bio-interactive properties. In this context, microscopy techniques such as Atomic Force Microscopy (AFM) are emerging as fundamental tools to deeply investigate morphology and structural properties at micro and sub-micrometric scale, in order to evaluate the in time relationship between physicochemical properties of biomaterials and biological response. In particular, AFM is not only a mere tool for screening surface topography, but may offer a significant contribution to understand surface and interface properties, thus concurring to the optimization of biomaterials performance, processes, physical and chemical properties at the micro and nanoscale. This is possible by capitalizing the recent discoveries in nanotechnologies applied to soft matter such as atomic force spectroscopy to measure surface forces through force curves. By tip-sample local interactions, several information can be collected such as elasticity, viscoelasticity, surface charge densities and wettability. This paper overviews recent developments in AFM technology and imaging techniques by remarking differences in operational modes, the implementation of advanced tools and their current application in biomaterials science, in terms of characterization of polymeric devices in different forms (i.e., fibres, films or particles).

show abstract

Section: Working Principles and Basic Toolsmentioning

confidence: 99%

Section: Working Principles and Basic Toolsmentioning

confidence: 99%

Atomic Force Microscopy: A Powerful Tool to Address Scaffold Design in Tissue Engineering

Marrese

Guarino

Ambrosio

2017

JFB

108

View full text Add to dashboard Cite

show abstract

“…The performances of biomaterials strongly depend on the bio-functionalization of the device surfaces. The design and realization of bio/non-bio interfaces with specific properties, such as chemical stability, wettability, and the immobilization ability of other biomolecules, are key features but at the same time often bottlenecks in bio-device development and optimization (De Stefano et al, 2011). Enzyme immobilization is the basis for the development of various biotechnology tools with applications in industrial biotransformations, diagnostics, and biosensing, and its realization has to address issues such as the loss of the enzyme and the maintenance of its stability.…”

Section: Introductionmentioning

confidence: 99%

Vmh2 hydrophobin as a tool for the development of “self‐immobilizing” enzymes for biosensing

Piscitelli

Pennacchio

Longobardi

et al. 2016

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

Self-assembling proteins forming amyloid fibrils are promising candidates for the fabrication of biomaterials, due to the chemical and mechanical stability of their structures. Among potential applications, their use as platforms for enzyme immobilization is rapidly gathering attention. In this work, we demonstrate that the production of the enzyme glutathione-S-transferase (GST) fused to the class I hydrophobin Vmh2 from Pleurotus ostreatus represents an invaluable tool for the development of self-immobilizing enzymes useful for high throughput analyses. The proposed immobilization strategy is versatile since it can be applied, in principle, to every recombinant protein able to refold from Escherichia coli inclusion bodies. A GST based biosensor has been developed to quantify toxic compounds, such as the pesticides molinate and captan, in aqueous environmental samples. The main advantages of this sensor include simplicity and speed of preparation, high sensitivity, reusability, and accuracy. Biotechnol. Bioeng. 2017;114: 46-52. © 2016 Wiley Periodicals, Inc.

show abstract

Self-Assembling Protein–Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding

Liu

Nevanen

Paananen

et al. 2018

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

A new method is demonstrated for preparing antifouling and low nonspecific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pretreatments. The surface-active protein hydrophobin was covalently modified with a controlled radical polymerization initiator and allowed to self-assemble as a monolayer on hydrophobic surfaces, followed by the preparation of antifouling surfaces by Cu(0)-mediated living radical polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA) performed in situ. By taking advantage of hydrophobins to achieve at the same time the immobilization of protein A, this approach allowed to prepare surfaces for IgG1 binding featuring greatly reduced nonspecific adsorption. The success of the surface modification strategy was investigated by contact angle, XPS, and AFM characterization, while the antifouling performance and the reduction of nonspecific binding were confirmed by QCM-D measurements.

show abstract

Organic-inorganic Interfaces for a New Generation of Hybrid Biosensors

Cited by 3 publications

References 38 publications

Atomic Force Microscopy: A Powerful Tool to Address Scaffold Design in Tissue Engineering

Atomic Force Microscopy: A Powerful Tool to Address Scaffold Design in Tissue Engineering

Vmh2 hydrophobin as a tool for the development of “self‐immobilizing” enzymes for biosensing

Self-Assembling Protein–Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding

Contact Info

Product

Resources

About