Organic–inorganic nanocrystal reductase to promote green asymmetric synthesis

Abstract

Geotrichum candidum acetophenone reductase (GcAPRD) nanocrystal reduces broad kinds of ketones to their corresponding (S)-alcohols with excellent enantioselectivity.

Recent Advances in Enzyme Immobilization Utilizing Nanotechnology for Biocatalysis

“…27 Some research reported that the affinity between the His tag on the proteins and the metal ions played a role in the nanocrystal formation. 28,47,48 2.2. Variability in the Nanocrystal Formation Process and Its Morphology.…”

“…An alcohol dehydrogenase (2 mg/mL) was immobilized with the nanocrystal formation method, and parallel hexahedron structures mixed with the spongelike structure were observed. 48 Furthermore, the metal ion in the nanocrystal formation process can be varied: for instance, Cu 2+ , Ca 2+ , Mn 2+ , Zn 2+ , Co 2+ , and Fe 2+ can be used. 15 54 Each metal ion used resulted in a different nanoflower structure and enzyme activity.…”

“…27,[36][37][38][39]41,42,44,50,51 Meanwhile, some enzymes are good with a particular metal ion. 47,48 Our group immobilized an alcohol dehydrogenase from Geotrichum candidum (GcAPRD) with various metal ions, including Mn 2+ , Mg 2+ , Ni 2+ , Ca 2+ , Fe 3+ , and Co 2+ . 48 We found that only Ni 2+ and Co 2+ could form nanocrystals with high immobilization yields and that only the nanocrystals made with Co 2+ retained sufficient activity.…”

“…47,48 Our group immobilized an alcohol dehydrogenase from Geotrichum candidum (GcAPRD) with various metal ions, including Mn 2+ , Mg 2+ , Ni 2+ , Ca 2+ , Fe 3+ , and Co 2+ . 48 We found that only Ni 2+ and Co 2+ could form nanocrystals with high immobilization yields and that only the nanocrystals made with Co 2+ retained sufficient activity. This was probably the case because the His tag of GcAPRD interacted well with only Ni 2+ and Co 2+ and the presence of Ni 2+ caused a decay of the GcAPRD activity.…”

Facile mussel-inspired polydopamine-coated 3D-printed bioreactors for continuous flow biocatalysis

“…[46][47][48][49][50][51][52] In our previous works, we immobilized GcAPRD with nanomaterials such as organic-inorganic nanocrystals and graphene-based nanomaterials. 53,54 However, flow process operation using the immobilized GcAPRD was not achieved, because the immobilized enzymes were too small to hold in the column in the flow process.…”