Organic management vs. conventional management influence the antimicrobial activity of essential oils of Origanum vulgare L

Pereira, Maysa Mathias Alves; Morais, Ludmila Caproni; Zeneratto, Natália Jubram; Reis, Willian de Souza Matias; Gómez, Omar Cabezas; Luiz, Jaine Honorata Hortolan; Moreira, Dajara Moana Barbosa; Pasqual, Moacir; Soares, Joyce Dória Rodrigues

doi:10.33448/rsd-v9i11.8504

Cited by 3 publications

(1 citation statement)

References 59 publications

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“…From molecular modeling studies, several terpenoids were potent inhibitors of SARS-CoV-2 replication ( 23 ). Among medicinal plants, Origanum vulgare has been recognized for its potential therapeutic role mainly arising from terpenes and flavonoids ( 24 , 25 ) and Thymus vulgaris EOs have been shown to be effective against several RNA viruses including coronaviruses ( 21 , 26 ). Regarding Cannabis sativa , it is particularly rich in terpenes, typically monoterpenes and sesquiterpenes ( 27 – 30 ).…”

Section: Introductionmentioning

confidence: 99%

Cannabidiol and Terpene Formulation Reducing SARS-CoV-2 Infectivity Tackling a Therapeutic Strategy

Santos¹,

Barata²,

Charmier³

et al. 2022

Front. Immunol.

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In late 2019, COVID-19 emerged in Wuhan, China. Currently, it is an ongoing global health threat stressing the need for therapeutic compounds. Linking the virus life cycle and its interaction with cell receptors and internal cellular machinery is key to developing therapies based on the control of infectivity and inflammation. In this framework, we evaluate the combination of cannabidiol (CBD), as an anti-inflammatory molecule, and terpenes, by their anti-microbiological properties, in reducing SARS-CoV-2 infectivity. Our group settled six formulations combining CBD and terpenes purified from Cannabis sativa L, Origanum vulgare, and Thymus mastichina. The formulations were analyzed by HPLC and GC-MS and evaluated for virucide and antiviral potential by in vitro studies in alveolar basal epithelial, colon, kidney, and keratinocyte human cell lines.Conclusions and ImpactWe demonstrate the virucide effectiveness of CBD and terpene-based formulations. F2TC reduces the infectivity by 17%, 24%, and 99% for CaCo-2, HaCat, and A549, respectively, and F1TC by 43%, 37%, and 29% for Hek293T, HaCaT, and Caco-2, respectively. To the best of our knowledge, this is the first approach that tackles the combination of CBD with a specific group of terpenes against SARS-CoV-2 in different cell lines. The differential effectiveness of formulations according to the cell line can be relevant to understanding the pattern of virus infectivity and the host inflammation response, and lead to new therapeutic strategies.

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Section: Introductionmentioning

confidence: 99%

Cannabidiol and Terpene Formulation Reducing SARS-CoV-2 Infectivity Tackling a Therapeutic Strategy

Santos¹,

Barata²,

Charmier³

et al. 2022

Front. Immunol.

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Antiviral and virucidal potential of Origanum vulgare Linn. (oregano) extracts against Bovine alphaherpesvirus 1 (BoHV-1)

Picoli

Waller

Hoffmann

et al. 2021

RSD

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The search for natural resources with antiviral potential, as an alternative to synthetic drugs, has been growing and, in this sense, oregano presents itself as a potential candidate. However, the antiviral studies with oregano are still poorly explored. BoHV-1 stands out among veterinary pathogens, for its economic impact on cattle production. In this study, the antiviral and virucidal activity of polar extracts of Origanum vulgare was evaluated against BoHV-1. Infusion (INF10), decoction (DEC), and hydroalcoholic (HAE) extracts were tested to cytotoxic and antiviral assays on MDBK cells. Cytotoxic effects were analyzed through MTT assay and the antiviral activity was expressed as a percentage of inhibition (PI). BoHV-1 was incubated with O. vulgare extracts as virucidal assay. Concentrations ≤3.12 mg/ml (INF10) and ≤1.56 mg/ml (DEC/HAE) preserved the cell viability above 60%, and all extracts were safe (>96%) between 0.78 and 0.39 mg/ml. Regarding the antiviral activity, pre-treatment of all extracts highlighted in comparison to the post-treatment. The pre-treatment of infusion at 2 mg/ml highlighted due to the high cell viability (84.69%) and the elimination of the viral load. All extracts inactivated BoHV-1 from 2 hours of incubation (20 mg/ml), showing virucidal activity. These findings may be related to 4-hydroxybenzoic acid as prevalent in all extracts. These findings showed the in vitro antiviral and virucidal activity of oregano polar extracts against BoHV-1 and may be promising for the therapeutic use against herpesviruses infections.

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Lamiaceae Familyasına Mensup Bazı Baharat Bitkilerinin Antimikrobiyal Etkilerinin Belirlenmesi

KEMER¹,

Akın

Saraçoğlu

2022

Selçuk Üniversitesi Fen Fakültesi Fen Dergisi

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Bu çalışma hidrodistilasyon yöntemi kullanılarak elde edilmiş olan Origanum vulgare, Salvia officinalis, Rosmarinus officinalis ve Mentha piperita bitkilerinin uçucu yağlarının Bacillus cereus ATCC 14579, Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923 mikroorganizmaları üzerindeki antimikrobiyal etkilerinin belirlenmesi amacıyla yapılmıştır. Antimikrobiyal etki mikrodilüsyon yöntemi kullanılarak tespit edilmiştir. Uçucu yağlara karşı en hassas bakterinin Escherichia coli ATCC 25922, en dirençli bakterinin ise Salmonella typhimurium ATCC 14028 olduğu tespit edilmiştir. Uçucu yağ konsantrasyonu düştükçe antimikrobiyal etkinin azaldığı da gözlemlenmiştir. En etkili uçucu yağın Origanum vulgare'ye ait olduğu ve yüksek antimikrobiyal etki gösterdiği belirlenmiştir. Rosmarinus officinalis ve Mentha piperita'nın Origanum vulgare'den sonra en etkili uçucu yağlar olduğu, bunu Salvia officinalis uçucu yağının izlediği tespit edilmiştir.

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Organic management vs. conventional management influence the antimicrobial activity of essential oils of Origanum vulgare L

Cited by 3 publications

References 59 publications

Cannabidiol and Terpene Formulation Reducing SARS-CoV-2 Infectivity Tackling a Therapeutic Strategy

Cannabidiol and Terpene Formulation Reducing SARS-CoV-2 Infectivity Tackling a Therapeutic Strategy

Antiviral and virucidal potential of Origanum vulgare Linn. (oregano) extracts against Bovine alphaherpesvirus 1 (BoHV-1)

Lamiaceae Familyasına Mensup Bazı Baharat Bitkilerinin Antimikrobiyal Etkilerinin Belirlenmesi

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