Organic mineral supplementation on differential protein profile of Osmanabadi bucks (Capra hircus)

Sekar, B. Chandra; Arunachalam, A.; Naidu, Sharanya Jeevendra; Reddy, I. J.; Bhatta, Raghavendra

doi:10.1016/j.repbio.2021.100533

Cited by 2 publications

(1 citation statement)

References 49 publications

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“…Immunoblotting: This is the continuation of previously published work in cryobiology (Arunachalam et al 2018, Mayasula et al 2022, B. Sekar et al 2021. The 2D Gel analysed differentially expressed protein localized and identified through MALDI-TOF (methodology followed as discussed by Mayasula et al 2022).…”

Section: Experimental Plan and 2 D Gel Electrophoresis Andmentioning

confidence: 88%

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Arunachalam

BACKIALAKSHMI

SHARANYA

et al. 2022

Indian J of Anim Sci

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The great expectation of the dairy owners from the frozen semen industry is quality semen with high fertility. Many researchers concentrate in this area to produce frozen semen of high quality and fertility. However, the problem still exists to minimize or reduce the cryoinjury related damages that occur during the process of cryopreservation with the use of all kinds of semen additives. The success rate of the additives added semen is variable at a larger scale. Therefore, any new molecules which can effectively prevent or minimize the cryoinjury are warranted and it could be low cost substances. Our incidental finding and protein identification with molecular weight ≈ 19 kDa (cold-inducible RNA-binding protein CIRBP) in goat spermatozoa seems to be effective in minimizing cryoinjury. The identified CIRBP was confirmed via Western blotting. In future, further studies are needed to confirm the molecular mechanism of the CIRBP on the cryoinjury as semen additives, and application of this protein added semen in the field to improve the level of conception rate in cattle.

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Section: Experimental Plan and 2 D Gel Electrophoresis Andmentioning

confidence: 88%

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Arunachalam

BACKIALAKSHMI

SHARANYA

et al. 2022

Indian J of Anim Sci

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Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks

BACKIALAKSHMI,

ARANGASAMY,

NAIDU

et al. 2024

Indian J of Anim Sci

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Sperm transcripts aside from providing genetic material have an eminent role in post-fertilization events and embryonic development. The current study was carried out to study the gene expression in Osmanabadi buck (Capra hircus) supplemented with organic minerals. Organic minerals alone or in combination with different concentrations were fed to the treatment groups (T2- Zn 20 mg, T3- Zn 40 mg, T4- Zn 60 mg, T5- Cu 12.5 mg, T6- Cu 25 mg, T7- Cu 37.5 mg, T8- Zn 20 mg + Cu 12.5 mg, T9- Zn 40 mg + Cu 25 mg, T10- Zn 60 mg + Cu 37.5 mg) along with the concentration mixture and roughages; whereas control (T1) group was fed concentrate mixture and roughages. The sperm samples were processed for gene expression studies of Ras Homolog Family Member A (RHOA), Mesoderm Specific Transcript (MEST), Nucleoside diphosphate-linked moiety X motif 6 (NUDT6), Protamine 1 (PRM1), Protamine 2 (PRM2) and Heat shock protein 90 alpha family class A member 1 (HSP90AA) using qRT-PCR and the protein-protein interaction analysis was carried out through String database version 11.0. Supplementation of trace minerals has enhanced the gene expression at a significant level (P<0.05) in the supplemented groups. The expression of MEST, RHOA, and PRM1 was higher in Cu 25-supplemented groups; whereas, NUDT6 in Zn 20, PRM2 and HSP90AA in Zn 40 showed higher expressions. Osmanabadi bucks supplemented with Cu 25 mg have shown promising results in the present and earlier studies so it can be concluded that the field-level application of Cu 25 mg would lead to promising results.

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Organic mineral supplementation on differential protein profile of Osmanabadi bucks (Capra hircus)

Cited by 2 publications

References 49 publications

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks

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