Organic N-fertilizer, rhizobacterial inoculation and fungal compost improve nutrient uptake, plant growth and the levels of vindoline, ajmalicine, vinblastine, catharanthine and total alkaloids in Catharanthus roseus L.

Abstract

The aim of the study was to replace mineral fertilizers with organic and biological fertilizers to improve nutrient uptake, plant growth and the concentrations of some important secondary metabolites in periwinkle (Catharanthus roseus L.). Periwinkle plants were grown under different rates of N supply (0, 20 and 40 mg kg -1 soil) and biological treatments (Azospirillum, Azotobacter, Azospirillum plus Azotobacter, Azospirillum plus fungal compost, Azotobacter plus fungal compost, and fungal compost). The concentrations of pigments and nutrients were measured by spectrophotometry and flame photometry. Secondary metabolites were analyzed by high-performance liquid chromatography (HPLC). Data were recorded for plant growth and development parameters, nutrient uptake and some secondary metabolites of periwinkle plants. The results showed that the N-fertilizer and biological treatments significantly improved most growth attributes and nutrient uptake and increased the concentrations of secondary metabolites as compared to the control. Maximum concentrations of root ajmalicine (0.54 mg g -1 DW), leaf vinblastine (0.96 mg g -1 DW) and root catharanthine (2.38 mg g -1 DW) were obtained from the treatment with Azospirillum under N-fertilizer at 20 and 40 mg kg -1 soil. Azotobacter along with fungal compost under N-fertilizer at 40 mg kg -1 soil induced the maximum concentration of leaf vindoline (1.94 mg g -1 DW). The highest concentration of root alkaloids (1.11 mg g -1 DW) was obtained from the treatment with compost under 40 mg N kg -1 soil. Azospirillum, Azotobacter and fungal compost combined with the N-fertilizer improved many morphological and nutrient characteristics. In conclusion, the growth and metabolism of C. roseus were significantly positively affected by the organic and biological fertilizers. Ke y wor d s: nitrogen fertilizer, periwinkle, plant secondary metabolites, root inoculation, Vinca rosea Organic N-fertilizer, rhizobacterial inoculation and fungal compost improve nutrient uptake, plant growth and the levels of vindoline, ajmalicine, vinblastine, catharanthine and total alkaloids in Catharanthus roseus L.

Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture

“…Monnerat et al [79] found that C. roseus plants supplied with nitrogen fertilizer in combinations with mycorrhizal fungi C. etunicatum, G. margarita and R. intraradices, showed increased production of ajmalicine. Hashemabadi et al [80] reported enhanced level of leaf vindoline (1.94 mg/g DW) and root alkaloid (1.11 mg/g DW) and other plant characteristics by application of 40 mg kg -1 soil nitrogen fertilizer along with Azotobacter and fungal compost. A positively significant effect of plant growth promoting rhizobacteria (PGPR) on the alkaloid content of C. roseus was reported recently [81][82][83][84].…”

Exploration of the Mechanisms of Differential Indole Alkaloid Biosynthesis in Dedifferentiated and Cambial Meristematic Cells of Catharanthus roseus Using Transcriptome Sequencing

“…The separation of the four compounds was achieved by using Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), by using 380 ml solution A [water: diethylamide = 986:14 (v/v), pH 7.2] combined with 620 ml solution B [methanol: acetonitrile = 4:1 (v/ v)] as the mobile phase. The detection was performed at a flow rate of 1.5 ml min-1 and a wavelength of 220 nm within 40 min as described earlier (Hashemabadi et al, 2018).…”

The Effect of Indigenous Azotobacter Isolate on Rice Results of Sri and Land Quality Methods

“…According to [26] the application of biological fertilizers containing various N-fixing bacteria can increase the morphological parameters of Catharanthus roseus L as well as secondary nutrient and metabolic absorption. The use of Arbuscular Mycorrhizal Fungi microbes in SRI method rice cultivation can increase SRI method rice production to 13.86 tons ha -1 [27]. Application of Bioorganic Plus containing Trichoderma harzianum and P. fluorescents microbes can increase the SRI method of rice production by 229.05% [28].…”